Source 1review2015Frontiers in Molecular Biosciences
Toolkit/hydrogen-deuterium exchange coupled to mass spectrometry
hydrogen-deuterium exchange coupled to mass spectrometry
Also known as: HDX-MS, hydrogen deuterium exchange
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) is an assay method used to complement structural characterization of light-activated photoreceptors. It reports on protein conformational dynamics in solution and can probe multiple functionally relevant states.
Usefulness & Problems
Why this is useful
HDX-MS is useful for characterizing solution-state conformational dynamics of photoreceptors beyond static structural descriptions. The cited review states that results from HDX-MS studies can help improve existing optogenetic systems or guide the design of novel optogenetic tools.
Source:
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Source:
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
Problem solved
This method addresses the need to interrogate conformational dynamics and multiple functionally relevant states of light-activated photoreceptors in solution. It specifically complements structural characterization when static structures alone are insufficient to describe functionally important state changes.
Source:
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Problem links
Need precise spatiotemporal control with light input
DerivedHydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) is an assay method used to complement structural characterization of light-activated photoreceptors. It reports on protein conformational dynamics in solution and can probe multiple functionally relevant states.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
conformational dynamics readoutconformational dynamics readoutConformational Uncaginghydrogen-deuterium exchangehydrogen-deuterium exchangeTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensorswitch architecture: uncaging
The evidence identifies HDX-MS as a mass spectrometry-coupled hydrogen-deuterium exchange assay applied to light-activated photoreceptors in solution. The provided sources do not specify construct design, sample preparation conditions, instrumentation settings, or expression and delivery requirements.
The supplied evidence does not provide quantitative performance metrics, resolution limits, or direct benchmarking against other structural methods. Independent validation across multiple tool-development settings is also not documented in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug hydrogen-deuterium-exchange-coupled-to-mass-spectrometry
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
Source:
application potentialsupports
Results from HDX-MS studies can help improve existing optogenetic systems or guide design of novel optogenetic tools.
We highlight recent examples demonstrating the potential of HDX-MS and discuss how these results can help to improve existing optogenetic systems or guide the design of novel optogenetic tools.
Source:
method capabilitysupports
HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution.
the ability of HDX-MS to provide information on conformational dynamics and the possibility to address multiple functionally relevant states in solution render this methodology ideally suitable
Source:
method rolesupports
HDX-MS can complement structural characterization of photoreceptors.
This review focuses on the potential of hydrogen-deuterium exchange coupled to mass spectrometry (HDX-MS) for complementing the structural characterization of photoreceptors.
Source:
Comparisons
Source-backed strengths
According to the cited review, HDX-MS provides information on conformational dynamics and can address multiple functionally relevant states in solution. Its stated strength in this context is as a complementary structural characterization method for photoreceptors.
Compared with atomic force sensing technique
hydrogen-deuterium exchange coupled to mass spectrometry and atomic force sensing technique address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging; same primary input modality: light
Compared with small-angle X-ray scattering
hydrogen-deuterium exchange coupled to mass spectrometry and small-angle X-ray scattering address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging; same primary input modality: light
Compared with temperature-dependent FTIR spectroscopy
hydrogen-deuterium exchange coupled to mass spectrometry and temperature-dependent FTIR spectroscopy address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging; same primary input modality: light
Ranked Citations
- 1.