Source 1primary paper2025MED
Toolkit/FIB-SEM tomography
FIB-SEM tomography
Also known as: FIB-SEM, focused ion beam milling and scanning electron microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
We use focused ion beam (FIB) milling and scanning electron microscopy (SEM) to directly evaluate changes in 3D pore structure in a Viresolve® Pro membrane due to fouling by human serum immunoglobulin G.
Usefulness & Problems
Why this is useful
FIB-SEM tomography is used here to directly evaluate 3D pore-structure changes in a virus removal membrane after protein fouling. The paper presents it as a way to visualize and quantify structural changes within the membrane.; direct evaluation of 3D pore-structure changes in virus removal membranes; quantifying effects of protein fouling on membrane morphology
Source:
FIB-SEM tomography is used here to directly evaluate 3D pore-structure changes in a virus removal membrane after protein fouling. The paper presents it as a way to visualize and quantify structural changes within the membrane.
Source:
direct evaluation of 3D pore-structure changes in virus removal membranes
Source:
quantifying effects of protein fouling on membrane morphology
Problem solved
It addresses the need to directly measure how protein fouling changes membrane pore structure rather than inferring those changes only from filtration performance.; provides direct structural measurement of fouling-induced pore changes in highly selective virus removal filters
Source:
It addresses the need to directly measure how protein fouling changes membrane pore structure rather than inferring those changes only from filtration performance.
Source:
provides direct structural measurement of fouling-induced pore changes in highly selective virus removal filters
Problem links
provides direct structural measurement of fouling-induced pore changes in highly selective virus removal filters
LiteratureIt addresses the need to directly measure how protein fouling changes membrane pore structure rather than inferring those changes only from filtration performance.
Source:
It addresses the need to directly measure how protein fouling changes membrane pore structure rather than inferring those changes only from filtration performance.
Published Workflows
Objective: Quantitatively analyze how protein fouling alters 3D pore structure and particle-capture behavior in a virus removal filtration membrane.
Why it works: The workflow combines direct 3D structural evaluation of the fouled membrane with flow and particle-transport simulations, allowing structural changes to be linked to permeability and capture-location behavior.
pore constriction from protein depositionredistribution of flow paths within the membranefocused ion beam millingscanning electron microscopyflow simulationparticle transport simulation
Stages
- 1.Direct 3D pore-structure evaluation of fouled membrane(functional_characterization)
This stage exists to directly measure how protein fouling changes the membrane structure.
Selection: Quantify changes in 3D pore structure caused by human serum immunoglobulin G fouling in the Viresolve® Pro membrane.
- 2.Flow and particle-transport simulation(secondary_characterization)
This stage exists to connect structural changes from fouling to transport behavior and capture-location shifts.
Selection: Use the protein-fouled membrane structure to simulate permeability and particle-capture behavior.
- 3.Comparison to independent experimental measurements(confirmatory_validation)
This stage exists to validate that the simulation results are consistent with independent experimental observations.
Selection: Assess whether simulation outputs match independent measurements of permeability and particle-capture location.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
focused ion beam millingscanning electron microscopy imagingthree-dimensional structural tomographyTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The method requires focused ion beam milling and scanning electron microscopy. In this study it is applied to a fouled Viresolve® Pro membrane challenged with human serum immunoglobulin G.; requires focused ion beam milling and scanning electron microscopy
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
FIB-SEM can directly evaluate changes in 3D pore structure in a Viresolve® Pro membrane caused by human serum immunoglobulin G fouling.
We use focused ion beam (FIB) milling and scanning electron microscopy (SEM) to directly evaluate changes in 3D pore structure in a Viresolve® Pro membrane due to fouling by human serum immunoglobulin G.
Approval Evidence
1 source1 linked approval claimfirst-pass slug fib-sem-tomography
We use focused ion beam (FIB) milling and scanning electron microscopy (SEM) to directly evaluate changes in 3D pore structure in a Viresolve® Pro membrane due to fouling by human serum immunoglobulin G.
Source:
method capabilitysupports
FIB-SEM can directly evaluate changes in 3D pore structure in a Viresolve® Pro membrane caused by human serum immunoglobulin G fouling.
We use focused ion beam (FIB) milling and scanning electron microscopy (SEM) to directly evaluate changes in 3D pore structure in a Viresolve® Pro membrane due to fouling by human serum immunoglobulin G.
Source:
Comparisons
Source-stated alternatives
The abstract contrasts this direct structural evaluation with model simulations and independent experimental measurements of permeability and particle-capture location.
Source:
The abstract contrasts this direct structural evaluation with model simulations and independent experimental measurements of permeability and particle-capture location.
Source-backed strengths
directly evaluates 3D pore structure; provides quantitative structural insight into fouling effects
Source:
directly evaluates 3D pore structure
Source:
provides quantitative structural insight into fouling effects
Compared with Langendorff perfused heart electrical recordings
FIB-SEM tomography and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with native green gel system
FIB-SEM tomography and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
FIB-SEM tomography and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.