Source 1primary paper2016Frontiers in Synaptic Neuroscience
Toolkit/flash-and-freeze
flash-and-freeze
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Flash-and-freeze is an assay method that induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing. It was developed to visualize activity-evoked synaptic membrane trafficking with millisecond temporal resolution and was used to identify ultrafast endocytosis during neurotransmission.
Usefulness & Problems
Why this is useful
This method is useful for capturing transient membrane-trafficking states at synapses that occur too rapidly for conventional fixation approaches. The source literature also states that the approach can be adapted to other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
Source:
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Problem solved
Flash-and-freeze addresses the problem of linking a precisely timed cellular stimulus to near-instantaneous structural preservation of membrane states. In the cited work, this enabled visualization of rapid membrane retrieval events following neurotransmission.
Problem links
We Can’t Take High-Resolution Movies of or Intervene in Brain Computation at the Single Neuron Level
Gap mapView gapThis technique is explicitly neuronal and captures membrane dynamics after light-triggered activity, so it is relevant to studying fast neural events. It is a weak fit for the stated gap because it is destructive and does not provide continuous high-resolution movies in vivo.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
light-triggered stimulationoptogenetic or light-triggered stimulationrapid cryo-immobilizationrapid cryo-immobilization of membrane statesTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The method requires a light flash to trigger neuronal activity and a rapid-freezing workflow to preserve membrane states immediately after stimulation. The source also indicates compatibility with light-sensitive genetic or pharmacological induction schemes, but the provided evidence does not specify particular actuators, wavelengths, or hardware configurations.
The supplied evidence describes the core concept and one discovery application, but provides limited detail on scope across cell types, molecular specificity, or quantitative performance metrics. Adaptability beyond synaptic membrane trafficking is claimed, yet the provided evidence does not document broad independent validation.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Approval Evidence
1 source3 linked approval claimsfirst-pass slug flash-and-freeze
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Source:
adaptabilitysupports
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
The flash-and-freeze approach can be adapted to study other cellular phenomena that can be induced by light-sensitive genetic or pharmacological tools.
Source:
discoverysupports
Using the flash-and-freeze approach, the authors characterized ultrafast endocytosis as a novel form of endocytosis that rapidly removes excess membrane added to the surface during neurotransmission.
Using this approach, we have characterized a novel form of endocytosis, ultrafast endocytosis, which rapidly removes excess membrane added to the surface during neurotransmission.
Source:
method capabilitysupports
Flash-and-freeze induces neuronal activity with a flash of light and captures membrane dynamics by rapid freezing.
we have developed a novel technique, called flash-and-freeze, which induces neuronal activity with a flash of light and captures the membrane dynamics by rapid freezing
Source:
Comparisons
Source-backed strengths
A key strength is the coordination of light-triggered stimulation with rapid cryo-immobilization, providing millisecond temporal resolution for membrane dynamics. The method supported the characterization of ultrafast endocytosis as a novel endocytic mode that rapidly removes excess membrane added during neurotransmission.
Compared with native green gel system
flash-and-freeze and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with open-source microplate reader
flash-and-freeze and open-source microplate reader address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with plant transcriptome profiling
flash-and-freeze and plant transcriptome profiling address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.