Source 1primary paper2015Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE
Toolkit/FLIPR
FLIPR
Also known as: Fluorometric Imaging Plate Reader
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
FLIPR (Fluorometric Imaging Plate Reader) is a fluorescence-analysis instrument used as a miniaturized optogenetic assay platform in 384-well plates. In the cited study, FLIPR LEDs provided optical modulation to support recombinant cellular assays, including Channelrhodopsin-2 control of CaV1.3.
Usefulness & Problems
Why this is useful
FLIPR is useful for adapting optogenetic cellular assays to high-throughput screening workflows that already rely on fluorescence plate-reader instrumentation. The cited work indicates that optical modulation and fluorescence-based assay operation can be combined in a 384-well miniaturized format.
Source:
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
Problem solved
This platform addresses the problem of implementing optogenetic modulation in a miniaturized high-throughput screening format rather than lower-throughput bespoke optical setups. The evidence specifically supports use of FLIPR to run recombinant cellular assays in 384-well plates with LED-based stimulation.
Source:
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
Problem links
Need better screening or enrichment leverage
DerivedFLIPR (Fluorometric Imaging Plate Reader) is a fluorescence-based high-throughput screening instrument used here as a miniaturized optogenetic assay platform in 384-well plates. The cited study used FLIPR LEDs to deliver optical modulation and to support recombinant cellular assays involving Channelrhodopsin-2/CaV1.3 and bPAC/HCN2 pairings.
Need conditional recombination or state switching
DerivedFLIPR (Fluorometric Imaging Plate Reader) is a fluorescence-based high-throughput screening instrument used here as a miniaturized optogenetic assay platform in 384-well plates. The cited study used FLIPR LEDs to deliver optical modulation and to support recombinant cellular assays involving Channelrhodopsin-2/CaV1.3 and bPAC/HCN2 pairings.
Need precise spatiotemporal control with light input
DerivedFLIPR (Fluorometric Imaging Plate Reader) is a fluorescence-based high-throughput screening instrument used here as a miniaturized optogenetic assay platform in 384-well plates. The cited study used FLIPR LEDs to deliver optical modulation and to support recombinant cellular assays involving Channelrhodopsin-2/CaV1.3 and bPAC/HCN2 pairings.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
fluorescence-based readoutfluorescence-based readoutoptical stimulationoptical stimulationTarget processes
recombinationselectionInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The available evidence supports use of FLIPR as instrumentation normally dedicated to fluorescence analysis in HTS activities and repurposed here for optogenetic optical modulation. Practical implementation details are limited to LED-based stimulation and 384-well plate format; the supplied evidence does not specify construct design, cell type, illumination parameters, or fluorophore requirements.
The evidence is limited to a single cited study and does not provide detailed quantitative performance metrics, wavelength specifications, or cross-platform benchmarking. Independent replication, assay generality across many targets, and operational constraints beyond the reported examples are not established in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
plate format 384-well
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
bPAC adenylyl cyclase was used to modulate the HCN2 cyclic nucleotide gated channel in an optogenetic assay.
the HCN2 cyclic nucleotide gated (CNG) channel was modulated by the light activated bPAC adenylyl cyclase
Channelrhodopsin-2 was used to modulate the CaV1.3 calcium channel in an optogenetic assay.
the CaV1.3 calcium channel was modulated by the light-activated Channelrhodopsin-2
Channelrhodopsin-2 was used to modulate the CaV1.3 calcium channel in an optogenetic assay.
the CaV1.3 calcium channel was modulated by the light-activated Channelrhodopsin-2
Channelrhodopsin-2 was used to modulate the CaV1.3 calcium channel in an optogenetic assay.
the CaV1.3 calcium channel was modulated by the light-activated Channelrhodopsin-2
Channelrhodopsin-2 was used to modulate the CaV1.3 calcium channel in an optogenetic assay.
the CaV1.3 calcium channel was modulated by the light-activated Channelrhodopsin-2
Channelrhodopsin-2 was used to modulate the CaV1.3 calcium channel in an optogenetic assay.
the CaV1.3 calcium channel was modulated by the light-activated Channelrhodopsin-2
Approval Evidence
1 source2 linked approval claimsfirst-pass slug flipr
using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
Source:
applicationsupports
Optical modulation for optogenetic assays can be obtained in a miniaturized 384-well plate format using the FLIPR instrument.
we sought to determine if this optical modulation can be obtained also in a miniaturized format, such as a 384-well plate, using the instrumentations normally dedicated to fluorescence analysis in High Throughput Screening (HTS) activities, such as for example the FLIPR (Fluorometric Imaging Plate Reader) instrument
Source:
assay performancesupports
Stable, robust, and miniaturized cellular assays can be developed using different optogenetic tools and modulated by FLIPR LEDs in a 384-well format.
stable, robust and miniaturized cellular assays can be developed using different optogenetic tools, and efficiently modulated by the FLIPR instrument LEDs in a 384-well format
Source:
Comparisons
Source-backed strengths
The cited study reports that stable, robust, and miniaturized cellular assays can be developed using optogenetic tools modulated by FLIPR LEDs in 384-well format. It also demonstrates at least one concrete assay pairing, with Channelrhodopsin-2 used to modulate the CaV1.3 calcium channel.
Compared with droplet microfluidic platform
FLIPR and droplet microfluidic platform address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Compared with fluorescence recovery after photobleaching
FLIPR and fluorescence recovery after photobleaching address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Compared with open-source microplate reader
FLIPR and open-source microplate reader address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Ranked Citations
- 1.