Source 1primary paper2025Chemical Science
Toolkit/glass nanopipette-based single-cell extraction
glass nanopipette-based single-cell extraction
Also known as: nanopipette-based single-cell extraction
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Glass nanopipette-based single-cell extraction is an ex situ single-cell sampling method that removes material from individual cells for downstream analysis. In the cited Chemical Science study, it was coupled to SiNx solid-state nanopores to identify LOV2 and monitor its conformational changes from single-cell extracts.
Usefulness & Problems
Why this is useful
This method is useful because it enables protein analysis from individual cells rather than pooled samples. The cited work specifically shows compatibility with SiNx solid-state nanopore readout for single-molecule protein detection and conformational state monitoring from single-cell extracts.
Problem solved
It addresses the problem of obtaining analyte from a single cell for downstream protein characterization. The reported application specifically solves access to LOV2-containing single-cell extracts for identification and conformational monitoring using solid-state nanopores.
Problem links
obtains single-cell extracts for ex situ protein measurement
LiteratureIt provides a way to obtain material from single cells for ex situ single-molecule protein measurement.
Source:
It provides a way to obtain material from single cells for ex situ single-molecule protein measurement.
Published Workflows
Exploring a solid-state nanopore approach for single-molecule protein detection from single cells
2025Objective: Develop and test a solid-state nanopore approach for direct single-molecule protein detection and conformational monitoring from single-cell extracts.
Why it works: The abstract states that a nanopore electrophoretic driver was fused to LOV2 to enhance capture efficiency, after which single-cell contents were extracted and analyzed with SiNx nanopores to identify the protein and monitor conformational changes.
electrophoretic-driver-assisted protein capturenanopore-based single-molecule protein sensingconformational change monitoringfusion construct designglass nanopipette-based single-cell extractionsolid-state nanopore measurement
Stages
- 1.Capture-enhancing fusion design(library_design)
The abstract states that the driver fusion was designed to enhance capture efficiency before nanopore analysis.
Selection: Design a nanopore electrophoretic driver protein fused to LOV2 to enhance target-protein capture efficiency.
- 2.Single-cell content extraction(functional_characterization)
This stage provides ex situ single-cell extracts for downstream nanopore protein analysis.
Selection: Directly extract contents of individual cells using glass nanopipette-based single-cell extraction.
- 3.Nanopore readout of single-cell extracts(confirmatory_validation)
This stage tests whether the overall approach can read out a model protein directly from single-cell extracts.
Selection: Use SiNx nanopores to identify LOV2 and monitor its conformational changes from single-cell extracts.
Steps
- 1.Design nanopore electrophoretic driver and fuse it to LOV2engineered fusion construct
Enhance capture efficiency of the target protein for nanopore analysis.
The abstract indicates this engineering step precedes single-cell analysis because improved capture is needed before nanopore measurement.
- 2.Extract contents of individual cells using glass nanopipette-based single-cell extractionsingle-cell sampling method
Obtain ex situ single-cell extracts for downstream nanopore protein analysis.
This step is performed after construct design to provide the single-cell material that will be analyzed by nanopores.
- 3.Use SiNx nanopores to identify LOV2 and monitor conformational changes from single-cell extractsnanopore analysis platform
Demonstrate direct single-molecule protein detection and conformational monitoring from single-cell extracts.
This is the downstream readout step applied after single-cell extraction to test the full approach on cellular material.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
Conformational Uncagingprotein conformational state monitoringsingle-cell content extractionsolid-state nanopore-based single-molecule detectionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensorswitch architecture: uncaging
The reported implementation uses a glass nanopipette for single-cell extraction and SiNx solid-state nanopores for downstream analysis. Beyond this coupling and the LOV2 application, the supplied evidence does not specify construct design, expression system, buffer conditions, or operational parameters.
The available evidence is limited to a single reported application involving LOV2 and SiNx solid-state nanopores. The provided evidence does not establish generality across other proteins, cell types, extraction yields, throughput, or quantitative recovery performance.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
SiNx solid-state nanopores can be used for single-molecule protein analysis from complex cellular samples.
Here, we explored the potential of SiN x solid-state nanopores for single-molecule protein analysis from complex cellular samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Proteins measured directly from single cells differed significantly from proteins obtained from purified samples.
Our results reveal significant differences between proteins measured directly from single cells and those obtained from purified samples.
Fusing a nanopore electrophoretic driver protein to LOV2 enhanced capture efficiency of the target protein.
we designed a nanopore electrophoretic driver protein and fused it with LOV2, thereby enhancing the capture efficiency of the target protein
Fusing a nanopore electrophoretic driver protein to LOV2 enhanced capture efficiency of the target protein.
we designed a nanopore electrophoretic driver protein and fused it with LOV2, thereby enhancing the capture efficiency of the target protein
Fusing a nanopore electrophoretic driver protein to LOV2 enhanced capture efficiency of the target protein.
we designed a nanopore electrophoretic driver protein and fused it with LOV2, thereby enhancing the capture efficiency of the target protein
Fusing a nanopore electrophoretic driver protein to LOV2 enhanced capture efficiency of the target protein.
we designed a nanopore electrophoretic driver protein and fused it with LOV2, thereby enhancing the capture efficiency of the target protein
Fusing a nanopore electrophoretic driver protein to LOV2 enhanced capture efficiency of the target protein.
we designed a nanopore electrophoretic driver protein and fused it with LOV2, thereby enhancing the capture efficiency of the target protein
Approval Evidence
1 source1 linked approval claimfirst-pass slug glass-nanopipette-based-single-cell-extraction
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction
Source:
application resultsupports
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Source:
Comparisons
Source-backed strengths
The main demonstrated strength is direct coupling of single-cell extraction to SiNx nanopore analysis. In the cited study, this combination was sufficient to identify LOV2 and detect its conformational changes from material extracted from individual cells.
Compared with Epitope analysis
glass nanopipette-based single-cell extraction and Epitope analysis address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging
Compared with pulsed laser-induced transient grating technique
glass nanopipette-based single-cell extraction and pulsed laser-induced transient grating technique address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging
Strengths here: looks easier to implement in practice.
Compared with single-molecule fluorescence resonance energy transfer
glass nanopipette-based single-cell extraction and single-molecule fluorescence resonance energy transfer address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: conformational_uncaging
Ranked Citations
- 1.