Source 1primary paper2025Chemical Science
Toolkit/glass nanopipette-based single-cell extraction
glass nanopipette-based single-cell extraction
Also known as: nanopipette-based single-cell extraction
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Glass nanopipette-based single-cell extraction is an ex situ single-cell sampling method that removes material from individual cells for downstream analysis. In the cited Chemical Science study, it was coupled to SiNx solid-state nanopores to identify LOV2 and monitor its conformational changes from single-cell extracts.
Usefulness & Problems
Why this is useful
This method is useful because it enables protein analysis from individual cells rather than pooled samples. The cited work specifically shows compatibility with SiNx solid-state nanopore readout for single-molecule protein detection and conformational state monitoring from single-cell extracts.
Problem solved
It addresses the problem of obtaining analyte from a single cell for downstream protein characterization. The reported application specifically solves access to LOV2-containing single-cell extracts for identification and conformational monitoring using solid-state nanopores.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
Conformational Uncagingprotein conformational state monitoringprotein conformational state monitoringsingle-cell content extractionsingle-cell content extractionsolid-state nanopore-based single-molecule detectionsolid-state nanopore-based single-molecule detectionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownex situ: Trueimplementation constraint: context specific validationoperating role: sensorsingle cell sampling: True
The reported implementation uses a glass nanopipette for single-cell extraction and SiNx solid-state nanopores for downstream analysis. Beyond this coupling and the LOV2 application, the supplied evidence does not specify construct design, expression system, buffer conditions, or operational parameters.
The available evidence is limited to a single reported application involving LOV2 and SiNx solid-state nanopores. The provided evidence does not establish generality across other proteins, cell types, extraction yields, throughput, or quantitative recovery performance.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Approval Evidence
1 source1 linked approval claimfirst-pass slug glass-nanopipette-based-single-cell-extraction
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction
Source:
application resultsupports
Using glass nanopipette-based single-cell extraction together with SiNx nanopores, the authors identified LOV2 and monitored its conformational changes from single-cell extracts.
we performed ex situ single-cell protein analysis by directly extracting the contents of individual cells using glass nanopipette-based single-cell extraction and successfully identified and monitored the conformational changes of the LOV2 protein from single-cell extracts using SiN x nanopores
Source:
Comparisons
Source-backed strengths
The main demonstrated strength is direct coupling of single-cell extraction to SiNx nanopore analysis. In the cited study, this combination was sufficient to identify LOV2 and detect its conformational changes from material extracted from individual cells.
Ranked Citations
- 1.