Source 1review2019Chemical Reviews
Toolkit/single-molecule fluorescence resonance energy transfer
single-molecule fluorescence resonance energy transfer
Also known as: smFRET
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we review a broad spectrum of single-molecule tools and techniques such as ... single-molecule fluorescence resonance energy transfer (smFRET)...
Usefulness & Problems
Why this is useful
smFRET is presented as a single-molecule technique used to study DNA motors by observing dynamic conformational changes. It is part of the review's core analytical toolkit.; single-molecule analysis of DNA motors; observing dynamic conformational changes
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smFRET is presented as a single-molecule technique used to study DNA motors by observing dynamic conformational changes. It is part of the review's core analytical toolkit.
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single-molecule analysis of DNA motors
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observing dynamic conformational changes
Problem solved
It helps reveal conformational dynamics and intermediates in motor reaction cycles.; enables observation of conformational changes in single motor complexes
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It helps reveal conformational dynamics and intermediates in motor reaction cycles.
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enables observation of conformational changes in single motor complexes
Problem links
enables observation of conformational changes in single motor complexes
LiteratureIt helps reveal conformational dynamics and intermediates in motor reaction cycles.
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It helps reveal conformational dynamics and intermediates in motor reaction cycles.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
Conformational Uncagingfluorescence resonance energy transfersingle-molecule conformational state reportingTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensorswitch architecture: uncaging
The abstract supports the need for a single-molecule fluorescence setup. Labeling or assay specifics are not described in the provided evidence.; requires single-molecule fluorescence measurement setup
The abstract does not specify whether smFRET alone provides force or torque manipulation, and it notes that hybrid techniques can increase observables.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The reviewed single-molecule methods enable manipulation of individual biomolecules through applied forces and torques and observation of dynamic conformational changes in single motor complexes.
Mutagenesis, chemical modifications, and optogenetics have been used to re-engineer existing molecular motors to alter speed, processivity, or functionality.
In-depth analysis of mechanochemical coupling in molecular motors has enabled development of artificially engineered motors.
Single-molecule techniques have been extensively used to identify structural intermediates in molecular motor reaction cycles and to understand how substeps in energy consumption drive transitions between intermediates.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug single-molecule-fluorescence-resonance-energy-transfer
Here, we review a broad spectrum of single-molecule tools and techniques such as ... single-molecule fluorescence resonance energy transfer (smFRET)...
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capability summarysupports
The reviewed single-molecule methods enable manipulation of individual biomolecules through applied forces and torques and observation of dynamic conformational changes in single motor complexes.
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review scope summarysupports
Single-molecule techniques have been extensively used to identify structural intermediates in molecular motor reaction cycles and to understand how substeps in energy consumption drive transitions between intermediates.
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Comparisons
Source-stated alternatives
The abstract names optical tweezers, magnetic tweezers, AFM, nanopore tweezers, and hybrid techniques as alternative or complementary methods.
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The abstract names optical tweezers, magnetic tweezers, AFM, nanopore tweezers, and hybrid techniques as alternative or complementary methods.
Source-backed strengths
supports observation of dynamic conformational changes in single motor complexes
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supports observation of dynamic conformational changes in single motor complexes
Compared with magnetic tweezers
The abstract names optical tweezers, magnetic tweezers, AFM, nanopore tweezers, and hybrid techniques as alternative or complementary methods.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports observation of dynamic conformational changes in single motor complexes.
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The abstract names optical tweezers, magnetic tweezers, AFM, nanopore tweezers, and hybrid techniques as alternative or complementary methods.
Compared with nanopore tweezers
The abstract names optical tweezers, magnetic tweezers, AFM, nanopore tweezers, and hybrid techniques as alternative or complementary methods.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports observation of dynamic conformational changes in single motor complexes.
Source:
The abstract names optical tweezers, magnetic tweezers, AFM, nanopore tweezers, and hybrid techniques as alternative or complementary methods.
Compared with optical tweezers
The abstract names optical tweezers, magnetic tweezers, AFM, nanopore tweezers, and hybrid techniques as alternative or complementary methods.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports observation of dynamic conformational changes in single motor complexes.
Source:
The abstract names optical tweezers, magnetic tweezers, AFM, nanopore tweezers, and hybrid techniques as alternative or complementary methods.
Ranked Citations
- 1.