Toolkit/haematoxylin-eosin stained histological sections

haematoxylin-eosin stained histological sections

Assay Method·Research·Since 2015

Also known as: conventional haematoxylin-eosin stained sections, H&E stained sections

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

The histology of the main organs was examined using conventional haematoxylin-eosin stained sections.

Usefulness & Problems

Why this is useful

This method examines the histology of major reproductive organs in Fasciola hepatica using conventional stained sections. In the review, it is used to identify lesions associated with triclabendazole exposure and resistance status.; examining histology of Fasciola hepatica reproductive organs; screening for drug-induced histopathological changes; supporting field-trial assessment of drug resistance

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This method examines the histology of major reproductive organs in Fasciola hepatica using conventional stained sections. In the review, it is used to identify lesions associated with triclabendazole exposure and resistance status.

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examining histology of Fasciola hepatica reproductive organs

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screening for drug-induced histopathological changes

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supporting field-trial assessment of drug resistance

Problem solved

It offers a convenient screening readout for drug-induced reproductive pathology and for comparing susceptible versus resistant isolates or field cases.; provides a convenient way to detect reproductive-tissue lesions associated with triclabendazole response

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It offers a convenient screening readout for drug-induced reproductive pathology and for comparing susceptible versus resistant isolates or field cases.

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provides a convenient way to detect reproductive-tissue lesions associated with triclabendazole response

Problem links

provides a convenient way to detect reproductive-tissue lesions associated with triclabendazole response

Literature

It offers a convenient screening readout for drug-induced reproductive pathology and for comparing susceptible versus resistant isolates or field cases.

Source:

It offers a convenient screening readout for drug-induced reproductive pathology and for comparing susceptible versus resistant isolates or field cases.

Published Workflows

Fasciola hepatica: Histology of the Reproductive Organs and Differential Effects of Triclabendazole on Drug-Sensitive and Drug-Resistant Fluke Isolates and on Flukes from Selected Field Cases

2015

Objective: Use reproductive-organ histology and apoptosis validation to distinguish triclabendazole-sensitive from triclabendazole-resistant Fasciola hepatica and to support field-trial resistance assessment.

Why it works: The workflow first establishes normal reproductive anatomy and histology so that drug-induced lesions can be recognized, then uses apoptosis labeling to validate that morphologically abnormal dividing cells are undergoing cell death. Comparing these readouts across susceptible and resistant isolates or field cases helps separate responsive from nonresponsive flukes.

apoptosis in actively dividing reproductive cellsmicrotubule-formation inhibitionfailure of ovigenesiswhole-mount staininghistological sectioning and H&E stainingin situ hybridisation apoptosis labelingcomparison across susceptible and resistant isolates and field cases

Stages

  1. 1.
    Establish normal reproductive-system morphology and histology(functional_characterization)

    The review explicitly states that the detailed normal description is provided to aid recognition of drug-induced lesions and to inform similar toxicological studies.

    Selection: Generate a baseline description of normal macroscopic arrangement and histological features of testes, ovary, vitelline tissue, Mehlis' gland, and uterus.

  2. 2.
    Screen treated flukes for reproductive histopathology(broad_screen)

    Histology is presented as a convenient screening method for representative samples in field trials and for testing efficacy of new products.

    Selection: Assess histological changes in reproductive organs after triclabendazole treatment across susceptible, resistant, and field-derived flukes.

  3. 3.
    Validate apoptosis in abnormal reproductive cells(confirmatory_validation)

    The review states that apoptosis validation was carried out using an in situ hybridisation method, indicating a confirmatory layer beyond morphology alone.

    Selection: Use in situ hybridisation labeling of endonuclease-induced DNA strand breaks to confirm apoptosis in morphologically abnormal cells.

  4. 4.
    Interpret resistance status and product efficacy from comparative pathology patterns(decision_gate)

    The review explicitly positions histology as useful for validating instances of drug resistance in field trials and for testing efficacy of new products against resistant and susceptible isolates.

    Selection: Compare lesion and apoptosis patterns between TCBZ-sensitive isolates, TCBZ-resistant isolates, suspected resistant field cases, and nonresistant field cases.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Target processes

recombinationselection

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor

It requires fluke tissue sections and haematoxylin-eosin staining. The review also emphasizes the need for baseline knowledge of normal organ histology to interpret lesions.; requires sectioned fluke tissue and conventional histological staining; interpretation depends on reference knowledge of normal reproductive histology

The abstract does not claim that histology alone fully replaces coprological testing or directly establishes molecular resistance mechanisms.; the abstract presents histology as a supplement to conventional coprological testing rather than a replacement

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1method positioningsupports2015Source 1needs review

Examination of reproductive-structure histopathology may supplement and augment conventional coprological testing and aid interpretation of TEM findings.

Claim 2method utilitysupports2015Source 1needs review

Histological techniques can conveniently screen representative fluke samples in field trials to help validate suspected triclabendazole resistance and can test efficacy of new products against resistant and susceptible isolates.

Approval Evidence

1 source2 linked approval claimsfirst-pass slug haematoxylin-eosin-stained-histological-sections
The histology of the main organs was examined using conventional haematoxylin-eosin stained sections.

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method positioningsupports

Examination of reproductive-structure histopathology may supplement and augment conventional coprological testing and aid interpretation of TEM findings.

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method utilitysupports

Histological techniques can conveniently screen representative fluke samples in field trials to help validate suspected triclabendazole resistance and can test efficacy of new products against resistant and susceptible isolates.

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Comparisons

Source-stated alternatives

The review states that histology may supplement and augment conventional coprological testing, and may aid interpretation of TEM findings.

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The review states that histology may supplement and augment conventional coprological testing, and may aid interpretation of TEM findings.

Source-backed strengths

described as having potential utility for conveniently screening representative samples of flukes in field trials; can be used to test efficacy of new products against drug-resistant and drug-susceptible isolates; provides reference criteria for drug-induced histopathological changes

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described as having potential utility for conveniently screening representative samples of flukes in field trials

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can be used to test efficacy of new products against drug-resistant and drug-susceptible isolates

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provides reference criteria for drug-induced histopathological changes

Compared with droplet microfluidic platform

haematoxylin-eosin stained histological sections and droplet microfluidic platform address a similar problem space because they share recombination, selection.

Shared frame: same top-level item type; shared target processes: recombination, selection

Strengths here: looks easier to implement in practice.

Compared with open-source microplate reader

haematoxylin-eosin stained histological sections and open-source microplate reader address a similar problem space because they share recombination, selection.

Shared frame: same top-level item type; shared target processes: recombination, selection

Strengths here: looks easier to implement in practice.

Compared with touchscreen-equipped operant conditioning chambers

haematoxylin-eosin stained histological sections and touchscreen-equipped operant conditioning chambers address a similar problem space because they share recombination, selection.

Shared frame: same top-level item type; shared target processes: recombination, selection

Ranked Citations

  1. 1.
    StructuralSource 1Pathogens2015Claim 1Claim 2

    Seeded from load plan for claim c3. Extracted from this source document.