Source 1primary paper2019Journal of Visualized Experiments
Toolkit/high-speed camera setup
high-speed camera setup
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
adjusting the sample composition based on measurements of the extrusion stability taken with a high-speed camera setup
Usefulness & Problems
Why this is useful
This setup measures extrusion stability during high-viscosity sample delivery. The resulting measurements are used to adjust sample composition.; measuring extrusion stability; guiding sample composition adjustment during extrusion optimization
Source:
This setup measures extrusion stability during high-viscosity sample delivery. The resulting measurements are used to adjust sample composition.
Source:
measuring extrusion stability
Source:
guiding sample composition adjustment during extrusion optimization
Problem solved
It provides a way to quantify extrusion behavior so sample composition can be tuned for stable flow.; provides measurements used to optimize extrusion stability
Source:
It provides a way to quantify extrusion behavior so sample composition can be tuned for stable flow.
Source:
provides measurements used to optimize extrusion stability
Problem links
provides measurements used to optimize extrusion stability
LiteratureIt provides a way to quantify extrusion behavior so sample composition can be tuned for stable flow.
Source:
It provides a way to quantify extrusion behavior so sample composition can be tuned for stable flow.
Published Workflows
Objective: Optimize extrusion of bacteriorhodopsin microcrystals for time-resolved serial femtosecond crystallography by achieving stable continuous flow while maintaining high crystal density and ensuring crystal clearance between pump laser pulses.
Why it works: The workflow first prepares lipidic cubic phase with a homogeneous crystal distribution and then adjusts sample composition using measured extrusion stability, which the abstract presents as the route to stable continuous flow at high crystal density.
homogeneous distribution of crystals in lipidic cubic phasesample composition adjustment to improve extrusion stabilityhigh-viscosity micro-extrusionthree-way syringe coupling preparationhigh-speed camera-based extrusion stability measurement
Stages
- 1.Prepare lipidic cubic phase with homogeneous crystal distribution(library_build)
This stage creates lipidic cubic phase with a homogeneous distribution of crystals before extrusion optimization.
Selection: homogeneous distribution of crystals in lipidic cubic phase
- 2.Measure extrusion stability and adjust sample composition(functional_characterization)
This stage uses high-speed camera measurements of extrusion stability to guide sample composition adjustment for optimized flow.
Selection: measurements of extrusion stability
Steps
- 1.Prepare lipidic cubic phase with homogeneous crystal distribution using the three-way syringe coupling devicesample-preparation device
Generate lipidic cubic phase with homogeneous crystal distribution before extrusion testing.
Homogeneous crystal distribution is prepared first because it is part of how the method achieves optimized extrusion.
- 2.Measure extrusion stability with a high-speed camera setupmeasurement setup
Obtain extrusion stability measurements to inform sample composition adjustment.
This measurement is performed before composition adjustment because the adjustment is based on the measured extrusion stability.
- 3.Adjust sample composition based on extrusion stability measurements
Tune the sample for stable continuous flow while maintaining high crystal density.
This step follows the measurement step because the composition adjustment is explicitly based on the measured extrusion stability.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
It requires a high-speed camera setup integrated with the extrusion test configuration.; requires a high-speed camera setup to measure extrusion stability
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The described methodology can be adapted to optimize the flow of other microcrystals.
The methodology can be adapted to optimize the flow of other microcrystals.
The method aims to optimize extrusion for stable continuous flow while maintaining high crystal density to increase TR-SFX data collection rate.
The goal of the method is to optimize extrusion for a stable and continuous flow while maintaining a high density of crystals to increase the rate at which data can be collected in a TR-SFX experiment.
High-viscosity micro-extrusion injectors dramatically reduce sample consumption in serial femtosecond crystallography experiments at XFELs.
High-viscosity micro-extrusion injectors have dramatically reduced sample consumption in serial femtosecond crystallographic experiments (SFX) at X-ray free electron lasers (XFELs).
High-viscosity micro-extrusion injectors are a preferred option for delivering crystals for time-resolved serial femtosecond crystallography in bacteriorhodopsin experiments.
A series of experiments using the light-driven proton pump bacteriorhodopsin have further established these injectors as a preferred option to deliver crystals for time-resolved serial femtosecond crystallography (TR-SFX) to resolve structural changes of proteins after photoactivation.
A high-speed camera setup is used to measure extrusion stability and guide sample composition adjustment.
followed by adjusting the sample composition based on measurements of the extrusion stability taken with a high-speed camera setup
A three-way syringe coupling device is used to prepare lipidic cubic phase with a homogeneous distribution of crystals.
We achieve this goal by preparing lipidic cubic phase with a homogenous distribution of crystals using a novel three-way syringe coupling device
Approval Evidence
1 source2 linked approval claimsfirst-pass slug high-speed-camera-setup
adjusting the sample composition based on measurements of the extrusion stability taken with a high-speed camera setup
Source:
generalizabilitysupports
The described methodology can be adapted to optimize the flow of other microcrystals.
The methodology can be adapted to optimize the flow of other microcrystals.
Source:
workflow component rolesupports
A high-speed camera setup is used to measure extrusion stability and guide sample composition adjustment.
followed by adjusting the sample composition based on measurements of the extrusion stability taken with a high-speed camera setup
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Comparisons
Source-stated alternatives
The abstract does not mention alternative measurement setups.
Source:
The abstract does not mention alternative measurement setups.
Source-backed strengths
supports measurement-based adjustment of sample composition
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supports measurement-based adjustment of sample composition
Compared with Langendorff perfused heart electrical recordings
high-speed camera setup and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with native green gel system
high-speed camera setup and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
high-speed camera setup and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.