Source 1primary paper2017Journal of Biotechnology
Toolkit/high-throughput online monitoring system with LED array
high-throughput online monitoring system with LED array
Also known as: high-throughput online monitoring system, LED array for individual illumination of every single well
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The high-throughput online monitoring system with an LED array is an assay platform for screening light-controlled gene expression conditions by individually illuminating each well in a multiwell format. In the cited yeast study, it was used with photocaged Cu2+ to regulate the Cu2+-inducible pCUP1 promoter from Saccharomyces cerevisiae and monitor eYFP expression.
Usefulness & Problems
Why this is useful
This platform is useful for parallel, automated testing of light-mediated induction conditions in gene expression experiments. The cited work presents caged Cu2+-based optical regulation as a minimally invasive, easy-to-control, temporal, and quantitative alternative to manual CuCl2 induction by pipetting.
Source:
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Source:
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
Problem solved
It addresses the need to control and compare induction timing and expression levels across many conditions in parallel for a light-responsive pCUP1 expression system in yeast. The evidence specifically supports its use for screening optical induction conditions rather than conventional chemical addition workflows.
Source:
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Problem links
This is one of the few explicit assay methods in the set, and the gap calls for better measurements and tests. It could plausibly support rapid screening of dynamic perturbation conditions or reporter responses, though the provided evidence is tied to an optogenetic induction setup rather than disease-causality studies.
Our Immune System Can Uniquely Recognize Nearly Any Molecule but We Don’t Know the Recognition Code
Gap mapView gapThe item is a high-throughput monitoring assay platform, so it could in principle support rapid screening campaigns needed to study recognition relationships. However, the supplied evidence ties it to induction/expression optimization rather than immune molecular recognition itself.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
light-mediated transcriptional inductionlight-mediated transcriptional inductionphotoinduced uncaging of cu2+photoinduced uncaging of cu2+Techniques
Functional AssayFunctional AssayFunctional AssaySelection / EnrichmentSelection / EnrichmentTarget processes
recombinationselectionImplementation Constraints
assay role: screening and monitoringcofactor dependency: cofactor requirement unknownencoding mode: genetically encodedillumination control: Trueimplementation constraint: context specific validationoperating role: sensorswitch architecture: uncaging
The documented application couples the LED-array monitoring system to photocaged Cu2+ release and the Cu2+-inducible pCUP1 promoter from Saccharomyces cerevisiae. Practical details beyond individual illumination of each well, such as LED wavelength, plate format, construct architecture, and instrumentation parameters, are not provided in the supplied evidence.
The available evidence is limited to a single cited study in yeast using a photocaged Cu2+-responsive pCUP1 system and eYFP readout. No independent replication, hardware performance specifications, wavelength details, throughput metrics, or validation in other organisms or promoters are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
Approval Evidence
1 source1 linked approval claimfirst-pass slug high-throughput-online-monitoring-system-with-led-array
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well.
Source:
screening resultsupports
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
Source:
Comparisons
Source-backed strengths
The reported advantages are parallelization, automation, temporal control, and quantitative control of induction conditions. Individual well illumination supports condition-resolved screening, and the associated caged Cu2+ strategy is described as minimally invasive and easy to control.
Source:
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Source:
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
Compared with chromatin in vivo imaging
high-throughput online monitoring system with LED array and chromatin in vivo imaging address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Compared with touchscreen-equipped operant conditioning chambers
high-throughput online monitoring system with LED array and touchscreen-equipped operant conditioning chambers address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Compared with whole genome screening of gene knockout mutants
high-throughput online monitoring system with LED array and whole genome screening of gene knockout mutants address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Ranked Citations
- 1.