Source 1primary paper2017Journal of Biotechnology
Toolkit/high-throughput online monitoring system with LED array
high-throughput online monitoring system with LED array
Also known as: high-throughput online monitoring system, LED array for individual illumination of every single well
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The high-throughput online monitoring system with an LED array is an assay platform for screening light-controlled gene expression conditions by individually illuminating each well in a multiwell format. In the cited yeast study, it was used with photocaged Cu2+ to regulate the Cu2+-inducible pCUP1 promoter from Saccharomyces cerevisiae and monitor eYFP expression.
Usefulness & Problems
Why this is useful
This platform is useful for parallel, automated testing of light-mediated induction conditions in gene expression experiments. The cited work presents caged Cu2+-based optical regulation as a minimally invasive, easy-to-control, temporal, and quantitative alternative to manual CuCl2 induction by pipetting.
Source:
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Source:
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
Problem solved
It addresses the need to control and compare induction timing and expression levels across many conditions in parallel for a light-responsive pCUP1 expression system in yeast. The evidence specifically supports its use for screening optical induction conditions rather than conventional chemical addition workflows.
Source:
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Problem links
enables automated parallel screening under individually controlled illumination conditions
LiteratureIt supports automated, parallel optimization of light-triggered expression conditions across many wells.
Source:
It supports automated, parallel optimization of light-triggered expression conditions across many wells.
Published Workflows
Objective: Develop and screen a light-controlled yeast gene-expression system based on photocaged Cu2+ release to regulate a Cu2+-inducible promoter and optimize induction timing and expression output.
Why it works: The workflow couples a photolabile Cu2+ source to a Cu2+-responsive promoter, so light exposure controls inducer availability and thereby transcription. High-throughput per-well illumination and monitoring allow screening for induction timing and expression level.
UV-A-triggered photorelease of Cu2+ from DMNP-EDTACu2+-induced activation of the pCUP1 promotercomputational chemistry supporthigh-throughput online monitoringindividual well LED illumination
Stages
- 1.Computationally supported system design(library_design)
The abstract states that computational chemistry supported the choice or use of the photolabile chelator for Cu2+ release.
Selection: Use of DMNP-EDTA as a photolabile chelator to enable Cu2+ release upon UV-A irradiation.
- 2.High-throughput screening of induction conditions(broad_screen)
This stage was used to identify favorable induction timing and expression output for the DMNP-EDTA-Cu system.
Selection: Best induction time and expression level of eYFP under individual well illumination.
Steps
- 1.Use DMNP-EDTA as a photolabile chelator for Cu2+ photoreleaseengineered inducible system
Create a light-responsive Cu2+ source that can drive a Cu2+-inducible promoter.
A photoreleasable Cu2+ input is required before optical control of the pCUP1 promoter can be tested.
- 2.Screen induction time and eYFP expression with per-well LED illumination and online monitoringsystem under test and screening platform
Identify the best induction time and expression level for the photocaged Cu2+ expression system.
After establishing a light-responsive Cu2+ control system, screening is needed to optimize operational conditions and quantify expression behavior.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Target processes
recombinationselectionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensorswitch architecture: uncaging
The documented application couples the LED-array monitoring system to photocaged Cu2+ release and the Cu2+-inducible pCUP1 promoter from Saccharomyces cerevisiae. Practical details beyond individual illumination of each well, such as LED wavelength, plate format, construct architecture, and instrumentation parameters, are not provided in the supplied evidence.
The available evidence is limited to a single cited study in yeast using a photocaged Cu2+-responsive pCUP1 system and eYFP readout. No independent replication, hardware performance specifications, wavelength details, throughput metrics, or validation in other organisms or promoters are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Photocaged Cu2+ release permits light-mediated control of the Cu2+-inducible pCUP1 promoter from S. cerevisiae.
This permits light-mediated control over the widely used Cu2+-inducible pCUP1 promoter from S. cerevisiae
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Caged Cu2+-based optical expression regulation provides minimally invasive, easy-to-control, parallel, automated, temporal, and quantitative control and is presented as a beneficial alternative to induction by pipetting CuCl2.
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The study reports the first example of a caged metal ion used to regulate recombinant gene expression.
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
DMNP-EDTA can be used to control Cu2+ release upon specific UV-A irradiation.
we used the versatile photolabile chelator DMNP-EDTA ... to control Cu2+ release upon specific UV-A irradiation
Approval Evidence
1 source1 linked approval claimfirst-pass slug high-throughput-online-monitoring-system-with-led-array
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well.
Source:
screening resultsupports
The DMNP-EDTA-Cu system was screened for optimal induction time and eYFP expression level using a high-throughput online monitoring system with individual well illumination.
We screened our novel DMNP-EDTA-Cu system for best induction time and expression level of eYFP with a high-throughput online monitoring system equipped with an LED array for individual illumination of every single well
Source:
Comparisons
Source-stated alternatives
The abstract does not name alternative screening platforms.
Source:
The abstract does not name alternative screening platforms.
Source-backed strengths
The reported advantages are parallelization, automation, temporal control, and quantitative control of induction conditions. Individual well illumination supports condition-resolved screening, and the associated caged Cu2+ strategy is described as minimally invasive and easy to control.
Source:
we realized a minimally invasive, easy-to-control, parallel and automated optical expression regulation via caged Cu2+ allowing temporal and quantitative control as a beneficial alternative to conventional induction via pipetting CuCl2 as effector molecule
Source:
thus constitutes the first example of a caged metal ion to regulate recombinant gene expression
Compared with haematoxylin-eosin stained histological sections
high-throughput online monitoring system with LED array and haematoxylin-eosin stained histological sections address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Compared with open-source microplate reader
high-throughput online monitoring system with LED array and open-source microplate reader address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Strengths here: looks easier to implement in practice.
Compared with touchscreen-equipped operant conditioning chambers
high-throughput online monitoring system with LED array and touchscreen-equipped operant conditioning chambers address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection
Ranked Citations
- 1.