Source 1review2015Cold Spring Harbor Perspectives in Biology
Toolkit/in situ microscopy
in situ microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The development of novel paradigms, model systems, and new tools in molecular genetics, electrophysiology, optogenetics, in situ microscopy, and functional imaging, have contributed markedly in recent years to our ability to investigate brain mechanisms of retrieval.
Usefulness & Problems
Why this is useful
In situ microscopy is listed as part of the methodological advances that improved the study of memory retrieval. It is presented as a way to investigate retrieval-related brain mechanisms alongside other molecular and systems tools.; investigating brain mechanisms of retrieval; examining neural representations involved in retrieval
Source:
In situ microscopy is listed as part of the methodological advances that improved the study of memory retrieval. It is presented as a way to investigate retrieval-related brain mechanisms alongside other molecular and systems tools.
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investigating brain mechanisms of retrieval
Source:
examining neural representations involved in retrieval
Problem solved
It contributes to overcoming the prior lack of methods for dissecting complex memory reactivation or reconstruction.; expands the methodological toolkit for studying retrieval-related brain processes
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It contributes to overcoming the prior lack of methods for dissecting complex memory reactivation or reconstruction.
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expands the methodological toolkit for studying retrieval-related brain processes
Problem links
expands the methodological toolkit for studying retrieval-related brain processes
LiteratureIt contributes to overcoming the prior lack of methods for dissecting complex memory reactivation or reconstruction.
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It contributes to overcoming the prior lack of methods for dissecting complex memory reactivation or reconstruction.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
light microscopyTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The abstract implies dependence on molecular and imaging preparations, but does not specify markers, microscopes, or sample types.; requires suitable microscopy-compatible preparations and molecular labeling strategies
The abstract does not establish that in situ microscopy alone can resolve the full fast interplay across distributed networks.; the abstract does not specify imaging targets, markers, or temporal resolution
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Recent development of novel paradigms, model systems, and tools in molecular genetics, electrophysiology, optogenetics, in situ microscopy, and functional imaging has markedly improved the ability to investigate brain mechanisms of memory retrieval.
Approval Evidence
1 source1 linked approval claimfirst-pass slug in-situ-microscopy
The development of novel paradigms, model systems, and new tools in molecular genetics, electrophysiology, optogenetics, in situ microscopy, and functional imaging, have contributed markedly in recent years to our ability to investigate brain mechanisms of retrieval.
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methodology enables studysupports
Recent development of novel paradigms, model systems, and tools in molecular genetics, electrophysiology, optogenetics, in situ microscopy, and functional imaging has markedly improved the ability to investigate brain mechanisms of memory retrieval.
Source:
Comparisons
Source-stated alternatives
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Source:
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Source-backed strengths
identified as one of the new tools contributing markedly to retrieval research
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identified as one of the new tools contributing markedly to retrieval research
Compared with electrophysiology
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: identified as one of the new tools contributing markedly to retrieval research.
Relative tradeoffs: the abstract does not specify imaging targets, markers, or temporal resolution.
Source:
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Compared with functional imaging
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: identified as one of the new tools contributing markedly to retrieval research.
Relative tradeoffs: the abstract does not specify imaging targets, markers, or temporal resolution.
Source:
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Compared with imaging
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: identified as one of the new tools contributing markedly to retrieval research.
Relative tradeoffs: the abstract does not specify imaging targets, markers, or temporal resolution.
Source:
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Compared with imaging surveillance
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: identified as one of the new tools contributing markedly to retrieval research.
Relative tradeoffs: the abstract does not specify imaging targets, markers, or temporal resolution.
Source:
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Compared with optogenetic functional interrogation
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: identified as one of the new tools contributing markedly to retrieval research.
Relative tradeoffs: the abstract does not specify imaging targets, markers, or temporal resolution.
Source:
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Compared with optogenetic membrane potential perturbation
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Shared frame: source-stated alternative in extracted literature
Strengths here: identified as one of the new tools contributing markedly to retrieval research.
Relative tradeoffs: the abstract does not specify imaging targets, markers, or temporal resolution.
Source:
Electrophysiology, optogenetics, and functional imaging are named as complementary alternatives.
Ranked Citations
- 1.