Toolkit/electrophysiology

electrophysiology

Assay Method·Research·Since 2022

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Electrophysiology is used as a functional assay in a multimodal study of gasdermin D pore behavior, alongside optogenetic tools and live-cell fluorescence biosensing. In the cited work, it supports measurement of pore conductance dynamics and the conclusion that gasdermin pores show phosphoinositide-dependent, repeated fast opening-closing behavior.

Usefulness & Problems

Why this is useful

This assay is useful for directly tracking functional pore activity rather than inferring pore state indirectly. In the cited study, it enabled analysis of dynamic gasdermin pore behavior within a light-enabled experimental framework that also included optogenetic perturbation and fluorescence biosensing.

Source:

Electrophysiology is used in the study to help demonstrate that gasdermin pores have phosphoinositide-dependent dynamics. The abstract supports its role in quantifying repeated fast opening-closing behavior.

Source:

measuring gasdermin pore opening-closing dynamics

Source:

probing pore activity on the tens of seconds timescale

Problem solved

It helps resolve whether gasdermin D pores behave as static open structures or undergo dynamic gating-like transitions. The supplied evidence indicates that electrophysiology contributed to showing repeated fast opening-closing events on the tens-of-seconds timescale and phosphoinositide-dependent dynamics.

Source:

It helps directly measure dynamic pore activity rather than assuming pores are permanently open.

Source:

provides direct functional readout of dynamic pore activity

Problem links

provides direct functional readout of dynamic pore activity

Literature

It helps directly measure dynamic pore activity rather than assuming pores are permanently open.

Source:

It helps directly measure dynamic pore activity rather than assuming pores are permanently open.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Target processes

No target processes tagged yet.

Input: Light

Implementation Constraints

application area: translational neurophysiologyapplication domain: neurogastroenterologyapplication domain: neuronal circuit analysiscofactor dependency: cofactor requirement unknowndomain: memory retrieval neuroscienceencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementmethod family: functional characterizationmodality: functional assaymodality: recordingmodality role: readoutnamed in abstract: Trueoperating role: sensorpaired method: optogeneticspaired with: fMRIreview context: stress neurobiologyreview emphasis: human electrographic findings and rodent electrophysiologytool class: complementary modality

The reported implementation combined electrophysiology with optogenetic tools and live-cell fluorescence biosensing. Light is part of the broader assay context, but the supplied evidence does not provide construct design, cell type, recording setup, or hardware details.

The supplied evidence does not specify the electrophysiological configuration, instrument subtype, recording mode, or quantitative performance metrics. It also does not show that electrophysiology alone identifies the underlying phosphoinositide circuit or visualizes pore structure.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1multimodal integrationsupports2025Source 2needs review

The review summarizes integration of calcium indicators with behavioral paradigms, electrophysiology, optogenetics, and chemogenetics to elucidate cellular and circuit mechanisms underlying depression.

Claim 2tool usage summarysupports2023Source 8needs review

The reviewed literature uses chemogenetic, optogenetic, genetic manipulation, electrophysiology, pharmacology, and immunohistochemistry approaches to investigate the role of specific cell subtypes in the stress response.

many studies have used state-of-the-art tools such as chemogenetic, optogenetic, genetic manipulation, electrophysiology, pharmacology, and immunohistochemistry to investigate the role of specific cell subtypes in the stress response
Claim 3application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 4application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 5application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 6application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 7application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 8application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 9application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 10application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 11application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 12application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 13application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 14application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 15application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 16application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 17application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 18application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 19application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 20application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 21application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 22application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 23application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 24application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 25application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 26application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 27application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 28application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 29application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 30application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 31application implicationsupports2022Source 6needs review

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.
Claim 32design considerationsupports2022Source 4needs review

Designing multimodal experiments that apply these tools within fMRI studies involves challenges and experimental choices.

Claim 33dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 34dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 35dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 36dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 37dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 38dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 39dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 40dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 41dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 42dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 43dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 44dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 45dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 46dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 47dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 48dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 49dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 50dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 51dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 52dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 53dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 54dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 55dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 56dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 57dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 58dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 59dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 60dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 61dynamic behaviorsupports2022Source 6needs review

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale
opening-closing timescale tens of seconds
Claim 62mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 63mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 64mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 65mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 66mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 67mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 68mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 69mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 70mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 71mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 72mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 73mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 74mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 75mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 76mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 77mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 78mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 79mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 80mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 81mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 82mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 83mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 84mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 85mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 86mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 87mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 88mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 89mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 90mechanistic findingsupports2022Source 6needs review

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.
Claim 91review scope summarysupports2022Source 4needs review

Multimodal neuroimaging that combines fMRI with calcium imaging, optogenetics, electrophysiology, or chemogenetics offers an opportunity to better understand brain function.

Claim 92application scopesupports2020Source 5needs review

Optogenetics and electrophysiology are being used to explore the brain-gut axis.

We discuss how these technologies and tools are currently being used to explore the brain-gut axis
Claim 93promise statementsupports2020Source 5needs review

The use of optogenetics and electrophysiology is presented as enabling researchers to answer important questions in neurogastroenterology through fundamental research.

Taken together, we consider that the use of these technologies will enable researchers to answer important questions in neurogastroenterology through fundamental research.
Claim 94review scopesupports2020Source 5needs review

The review focuses on optogenetics combined with electrophysiology in neurogastroenterology.

This review focuses on the use of optogenetics combined with electrophysiology in the field of neurogastroenterology.
Claim 95translational relevancesupports2020Source 5needs review

Answers generated using these technologies may shorten the path from basic discovery to new treatments for disorders of the brain-gut axis affecting the GI tract.

The answers to those questions will shorten the path from basic discovery to new treatments for patient populations with disorders of the brain-gut axis affecting the GI tract such as irritable bowel syndrome (IBS), functional dyspepsia, achalasia, and delayed gastric emptying.
Claim 96review summarysupports2017Source 3needs review

Behavioral and neurochemical strategies in this area require greater use of neurophysiological tools to better inform clinical research.

These strategies will require, however, a greater use of neurophysiological tools to better inform clinical research.
Claim 97review summarysupports2017Source 3needs review

Electrophysiology and viral vector-based circuit dissection such as optogenetics can further elucidate how exogenous cannabinoids worsen or ameliorate schizophrenia symptoms.

electrophysiology and viral vector-based circuit dissection, like optogenetics, can further elucidate how exogenous cannabinoids worsen (e.g., tetrahydrocannabinol, THC) or ameliorate (e.g., cannabidiol, CBD) schizophrenia symptoms
Claim 98methodology enables studysupports2015Source 9needs review

Recent development of novel paradigms, model systems, and tools in molecular genetics, electrophysiology, optogenetics, in situ microscopy, and functional imaging has markedly improved the ability to investigate brain mechanisms of memory retrieval.

Claim 99methodological impactsupports2013Source 7needs review

Recent advances in mouse genetics, electrophysiology, and optogenetic techniques have greatly contributed to improving understanding of homeostatic energy-balance regulation.

Approval Evidence

10 sources15 linked approval claimsfirst-pass slug electrophysiology
This review systematically summarizes the evolution of calcium indicators and their integration with behavioral paradigms, electrophysiology, optogenetics, and chemogenetics to elucidate cellular and circuit mechanisms underlying depression.

Source:

Using calcium-dependent fiber photometry, electrophysiology, and chemogenetic and optogenetic manipulations across learning paradigms, we explore the functions of VTADA neuronal activity during sleep.

Source:

This specifically focuses on neurophysiological function and dysfunction observed within these animal models, typically measured using electrophysiology or calcium imaging.

Source:

many studies have used state-of-the-art tools such as ... electrophysiology ... to investigate the role of specific cell subtypes in the stress response

Source:

Being able to combine calcium imaging, optogenetics, electrophysiology, chemogenetics, and functional magnetic resonance imaging (fMRI) as part of the numerous efforts on brain functional mapping, we have a unique opportunity to better understand brain function.

Source:

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.

Source:

This review focuses on the use of optogenetics combined with electrophysiology in the field of neurogastroenterology.

Source:

These strategies will require, however, a greater use of neurophysiological tools to better inform clinical research. In this sense, electrophysiology and viral vector-based circuit dissection, like optogenetics, can further elucidate how exogenous cannabinoids worsen or ameliorate schizophrenia symptoms

Source:

The development of novel paradigms, model systems, and new tools in molecular genetics, electrophysiology, optogenetics, in situ microscopy, and functional imaging, have contributed markedly in recent years to our ability to investigate brain mechanisms of retrieval.

Source:

In this article, we review current knowledge on the homeostatic regulation of energy balance, emphasizing recent advances in mouse genetics, electrophysiology, and optogenetic techniques that have greatly contributed to improving our understanding of this central process.

Source:

multimodal integrationsupports

The review summarizes integration of calcium indicators with behavioral paradigms, electrophysiology, optogenetics, and chemogenetics to elucidate cellular and circuit mechanisms underlying depression.

Source:

tool usage summarysupports

The reviewed literature uses chemogenetic, optogenetic, genetic manipulation, electrophysiology, pharmacology, and immunohistochemistry approaches to investigate the role of specific cell subtypes in the stress response.

many studies have used state-of-the-art tools such as chemogenetic, optogenetic, genetic manipulation, electrophysiology, pharmacology, and immunohistochemistry to investigate the role of specific cell subtypes in the stress response

Source:

application implicationsupports

Identification of the local phosphoinositide circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

The identification of this circuit allows pharmacological tuning of pyroptosis and control of inflammatory cytokine release by living cells.

Source:

design considerationsupports

Designing multimodal experiments that apply these tools within fMRI studies involves challenges and experimental choices.

Source:

dynamic behaviorsupports

Gasdermin pores undergo repeated fast opening-closing on the tens of seconds timescale.

We quantify repeated and fast opening-closing of these pores on the tens of seconds timescale

Source:

mechanistic findingsupports

Gasdermin pores display phosphoinositide-dependent dynamics.

Here, we combine optogenetic tools, live cell fluorescence biosensing, and electrophysiology to demonstrate that gasdermin pores display phosphoinositide-dependent dynamics.

Source:

review scope summarysupports

Multimodal neuroimaging that combines fMRI with calcium imaging, optogenetics, electrophysiology, or chemogenetics offers an opportunity to better understand brain function.

Source:

application scopesupports

Optogenetics and electrophysiology are being used to explore the brain-gut axis.

We discuss how these technologies and tools are currently being used to explore the brain-gut axis

Source:

promise statementsupports

The use of optogenetics and electrophysiology is presented as enabling researchers to answer important questions in neurogastroenterology through fundamental research.

Taken together, we consider that the use of these technologies will enable researchers to answer important questions in neurogastroenterology through fundamental research.

Source:

review scopesupports

The review focuses on optogenetics combined with electrophysiology in neurogastroenterology.

This review focuses on the use of optogenetics combined with electrophysiology in the field of neurogastroenterology.

Source:

translational relevancesupports

Answers generated using these technologies may shorten the path from basic discovery to new treatments for disorders of the brain-gut axis affecting the GI tract.

The answers to those questions will shorten the path from basic discovery to new treatments for patient populations with disorders of the brain-gut axis affecting the GI tract such as irritable bowel syndrome (IBS), functional dyspepsia, achalasia, and delayed gastric emptying.

Source:

review summarysupports

Behavioral and neurochemical strategies in this area require greater use of neurophysiological tools to better inform clinical research.

These strategies will require, however, a greater use of neurophysiological tools to better inform clinical research.

Source:

review summarysupports

Electrophysiology and viral vector-based circuit dissection such as optogenetics can further elucidate how exogenous cannabinoids worsen or ameliorate schizophrenia symptoms.

electrophysiology and viral vector-based circuit dissection, like optogenetics, can further elucidate how exogenous cannabinoids worsen (e.g., tetrahydrocannabinol, THC) or ameliorate (e.g., cannabidiol, CBD) schizophrenia symptoms

Source:

methodology enables studysupports

Recent development of novel paradigms, model systems, and tools in molecular genetics, electrophysiology, optogenetics, in situ microscopy, and functional imaging has markedly improved the ability to investigate brain mechanisms of memory retrieval.

Source:

methodological impactsupports

Recent advances in mouse genetics, electrophysiology, and optogenetic techniques have greatly contributed to improving understanding of homeostatic energy-balance regulation.

Source:

Comparisons

Source-stated alternatives

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Source:

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Source-backed strengths

The cited study used electrophysiology in combination with optogenetic tools and live-cell fluorescence biosensing, providing functional readout within a multimodal assay design. The evidence supports its value for detecting dynamic pore conductance behavior, including repeated fast opening-closing events and phosphoinositide dependence.

Source:

supports quantification of repeated and fast opening-closing events

Compared with biosensing

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Shared frame: source-stated alternative in extracted literature

Strengths here: supports quantification of repeated and fast opening-closing events.

Relative tradeoffs: the abstract does not specify the electrophysiology configuration or protocol.

Source:

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Compared with CRY2-CIB1 receptor optogenetic activation system

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Shared frame: source-stated alternative in extracted literature

Strengths here: supports quantification of repeated and fast opening-closing events.

Relative tradeoffs: the abstract does not specify the electrophysiology configuration or protocol.

Source:

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Compared with cryptochrome 2-mediated optogenetic C-RAF activation tool

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Shared frame: source-stated alternative in extracted literature

Strengths here: supports quantification of repeated and fast opening-closing events.

Relative tradeoffs: the abstract does not specify the electrophysiology configuration or protocol.

Source:

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Compared with DNMT3A-CRY2/CIB1-TRF1 optogenetic telomere-targeting fusion construct system

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Shared frame: source-stated alternative in extracted literature

Strengths here: supports quantification of repeated and fast opening-closing events.

Relative tradeoffs: the abstract does not specify the electrophysiology configuration or protocol.

Source:

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Compared with LOV-based optogenetic devices

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Shared frame: source-stated alternative in extracted literature

Strengths here: supports quantification of repeated and fast opening-closing events.

Relative tradeoffs: the abstract does not specify the electrophysiology configuration or protocol.

Source:

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Compared with optogenetic

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Shared frame: source-stated alternative in extracted literature

Strengths here: supports quantification of repeated and fast opening-closing events.

Relative tradeoffs: the abstract does not specify the electrophysiology configuration or protocol.

Source:

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Compared with optogenetic tool

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Shared frame: source-stated alternative in extracted literature

Strengths here: supports quantification of repeated and fast opening-closing events.

Relative tradeoffs: the abstract does not specify the electrophysiology configuration or protocol.

Source:

Live cell fluorescence biosensing and optogenetic tools are used alongside electrophysiology in the reported approach.

Ranked Citations

  1. 1.
    StructuralSource 1Frontiers in Synaptic Neuroscience2023

    Extracted from this source document.

  2. 2.
    StructuralSource 2Cell Calcium2025Claim 1

    Seeded from load plan for claim cl4. Extracted from this source document.

  3. 3.
    StructuralSource 3Frontiers in Pharmacology2017Claim 96Claim 97

    Extracted from this source document. Seeded from load plan for claim cl2.

  4. 4.
    StructuralSource 4Frontiers in Neuroscience2022Claim 32Claim 91

    Seeded from load plan for claim cl1. Extracted from this source document.

  5. 5.
    StructuralSource 5American Journal of Physiology-Gastrointestinal and Liver Physiology2020Claim 92Claim 93Claim 94

    Seeded from load plan for claim clm_1. Extracted from this source document.

  6. 6.
    StructuralSource 6Nature Communications2022Claim 28Claim 24Claim 24

    Extracted from this source document.

  7. 7.
    StructuralSource 7Journal of Endocrinology2013Claim 99

    Extracted from this source document. Seeded from load plan for claim cl3.

  8. 8.
    StructuralSource 8Frontiers in Neuroscience2023Claim 2

    Extracted from this source document. Seeded from load plan for claim cl2.

  9. 9.
    StructuralSource 9Cold Spring Harbor Perspectives in Biology2015Claim 98

    Seeded from load plan for claim cl_1. Extracted from this source document.

  10. 10.
    StructuralSource 10MED2025

    Extracted from this source document.