Toolkit/Iris

Iris

Assay Method·Research·Since 2016

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Iris is an intuitive web tool for programming light signals in optogenetics and photobiology experiments. In the supplied evidence, it is linked to programming illumination for BphP1-QPAS1-based systems, including the iRIS platform for light-controlled protein localization.

Usefulness & Problems

Why this is useful

Iris is useful because it provides a software interface for specifying light input patterns used to drive optogenetic and photobiology experiments. The evidence specifically connects it to experiments with BphP1-QPAS1-based tools in mammalian cells, where controlled illumination is required for transcriptional regulation and protein targeting workflows.

Source:

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.

Source:

and simplify the entrainment of cyanobacterial circadian rhythm.

Source:

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells

Source:

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Problem solved

Iris addresses the practical problem of programming experimental light signals for optogenetic stimulation. In the supplied evidence, this is relevant to operating near-infrared and blue-light-responsive BphP1-QPAS1 systems such as iRIS and the TA system.

Source:

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.

Source:

and simplify the entrainment of cyanobacterial circadian rhythm.

Source:

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells

Problem links

Need inducible protein relocalization or recruitment

Derived

Iris is an intuitive web tool for programming light signals in optogenetics and photobiology experiments. In the supplied evidence, it is linked to programming illumination for BphP1-QPAS1-based systems, including the iRIS platform for light-controlled protein localization.

Need precise spatiotemporal control with light input

Derived

Iris is an intuitive web tool for programming light signals in optogenetics and photobiology experiments. In the supplied evidence, it is linked to programming illumination for BphP1-QPAS1-based systems, including the iRIS platform for light-controlled protein localization.

Need tighter control over gene expression timing or amplitude

Derived

Iris is an intuitive web tool for programming light signals in optogenetics and photobiology experiments. In the supplied evidence, it is linked to programming illumination for BphP1-QPAS1-based systems, including the iRIS platform for light-controlled protein localization.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Target processes

localizationtranscription

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: sensor

The evidence indicates that Iris is a web tool used to program light signals, but it does not specify file formats, device interfaces, or control architecture. In the associated biological use case, it was used with BphP1-QPAS1-based optogenetic systems, and the small size of QPAS1 enabled AAV design for neuronal delivery of the TA system.

The supplied evidence does not report quantitative performance metrics, supported hardware compatibility details, or benchmarking against other light-programming software. Applicability of the associated BphP1-QPAS1 tools can depend on cell-type-specific physiology such as nuclear transport, and the blue-light-sensitive component of iRIS may be limited in some contexts.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 2application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 3application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 4application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 5application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 6application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 7application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 8cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 9cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 10cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 11cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 12cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 13cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 14cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 15delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 16delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 17delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 18delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 19delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 20delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 21delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 22performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 23performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 24performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 25performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 26performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 27performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 28performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 29performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 30performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 31performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 32performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 33performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 34performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 35performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 36accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 37accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 38accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 39accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 40accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 41accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 42accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 43accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 44accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 45accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 46accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 47accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 48accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 49accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 50application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 51application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 52application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 53application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 54application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 55application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 56application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 57application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 58application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 59application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 60application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 61application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 62application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 63application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 64application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 65application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 66application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 67application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 68application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 69application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 70application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 71application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 72application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 73application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 74application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 75application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 76application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 77application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 78capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 79capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 80capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 81capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 82capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 83capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 84capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 85engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 86engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 87engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 88engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 89engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 90engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 91engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 92impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 93impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 94impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 95impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 96impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 97impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 98impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 99impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 100impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 101impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 102impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 103impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 104impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 105impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 106programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 107programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 108programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 109programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 110programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 111programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 112programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 113programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 114programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 115programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 116programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 117programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 118programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 119programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.

Approval Evidence

2 sources5 linked approval claimsfirst-pass slug iris
two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS)

Source:

Signals are programmed using an intuitive web tool named Iris.

Source:

application scopesupports

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.

Source:

cell type dependencesupports

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.

Source:

performancesupports

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.

Source:

programming interfacesupports

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.

Source:

programming interfacesupports

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.

Source:

Comparisons

Source-backed strengths

The main supported strength is that Iris is described as an intuitive web tool for programming signals. Its relevance is reinforced by use in studies involving BphP1-QPAS1-based optogenetic tools tested across several mammalian cell types, including cortical neurons.

Source:

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.

Source:

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.

Source:

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Compared with direct stochastic optical reconstruction microscopy

Iris and direct stochastic optical reconstruction microscopy address a similar problem space because they share localization.

Shared frame: same top-level item type; shared target processes: localization; same primary input modality: light

Relative tradeoffs: appears more independently replicated.

Compared with open-source microplate reader

Iris and open-source microplate reader address a similar problem space because they share transcription.

Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light

Strengths here: appears more independently replicated; looks easier to implement in practice.

Compared with super-resolution cryogenic correlative light and electron microscopy

Iris and super-resolution cryogenic correlative light and electron microscopy address a similar problem space because they share localization.

Shared frame: same top-level item type; shared target processes: localization; same primary input modality: light

Strengths here: appears more independently replicated; looks easier to implement in practice.

Ranked Citations

  1. 1.
    StructuralSource 1Scientific Reports2016Claim 36Claim 37Claim 38

    Extracted from this source document.

  2. 2.
    StructuralSource 2ChemBioChem2018Claim 1Claim 2Claim 3

    Extracted from this source document.