Toolkit/Iris

Iris

Assay Method·Research·Since 2016

Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Iris is an intuitive web tool for programming light signals in optogenetics and photobiology experiments. In the supplied evidence, it is linked to programming illumination for BphP1-QPAS1-based systems, including the iRIS platform for light-controlled protein localization.

Usefulness & Problems

Why this is useful

Iris is useful because it provides a software interface for specifying light input patterns used to drive optogenetic and photobiology experiments. The evidence specifically connects it to experiments with BphP1-QPAS1-based tools in mammalian cells, where controlled illumination is required for transcriptional regulation and protein targeting workflows.

Source:

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.

Source:

and simplify the entrainment of cyanobacterial circadian rhythm.

Source:

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells

Source:

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Problem solved

Iris addresses the practical problem of programming experimental light signals for optogenetic stimulation. In the supplied evidence, this is relevant to operating near-infrared and blue-light-responsive BphP1-QPAS1 systems such as iRIS and the TA system.

Source:

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.

Source:

and simplify the entrainment of cyanobacterial circadian rhythm.

Source:

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells

Problem links

Need inducible protein relocalization or recruitment

Derived

Iris is an intuitive web tool for programming light signals in optogenetics and photobiology experiments. In the supplied evidence, it is associated with programming signals for BphP1-QPAS1-based optogenetic systems, including iRIS for light-controlled protein localization.

Need precise spatiotemporal control with light input

Derived

Iris is an intuitive web tool for programming light signals in optogenetics and photobiology experiments. In the supplied evidence, it is associated with programming signals for BphP1-QPAS1-based optogenetic systems, including iRIS for light-controlled protein localization.

Need tighter control over gene expression timing or amplitude

Derived

Iris is an intuitive web tool for programming light signals in optogenetics and photobiology experiments. In the supplied evidence, it is associated with programming signals for BphP1-QPAS1-based optogenetic systems, including iRIS for light-controlled protein localization.

Taxonomy & Function

Primary hierarchy

Technique Branch

Method: A concrete measurement method used to characterize an engineered system.

Target processes

localizationtranscription

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenimplementation constraint: payload burdenimplementation constraint: spectral hardware requirementoperating role: builderoperating role: regulatoroperating role: sensorswitch architecture: multi component

The evidence indicates that Iris is a web tool used to program light signals, but it does not specify file formats, device interfaces, or control architecture. In the associated biological use case, it was used with BphP1-QPAS1-based optogenetic systems, and the small size of QPAS1 enabled AAV design for neuronal delivery of the TA system.

The supplied evidence does not report quantitative performance metrics, supported hardware compatibility details, or benchmarking against other light-programming software. Applicability of the associated BphP1-QPAS1 tools can depend on cell-type-specific physiology such as nuclear transport, and the blue-light-sensitive component of iRIS may be limited in some contexts.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 2application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 3application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 4application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 5application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 6application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 7application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 8application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 9application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 10application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 11application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 12application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 13application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 14application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 15application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 16application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 17application scopesupports2018Source 2needs review

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.
Claim 18cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 19cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 20cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 21cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 22cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 23cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 24cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 25cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 26cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 27cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 28cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 29cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 30cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 31cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 32cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 33cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 34cell type dependencesupports2018Source 2needs review

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.
Claim 35delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 36delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 37delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 38delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 39delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 40delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 41delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 42delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 43delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 44delivery enabling propertysupports2018Source 2needs review

The small size of QPAS1 enabled design of AAV particles for delivery of the TA system to neurons.

The small size of the QPAS1 component allowed the design of adeno-associated virus (AAV) particles, which were applied to deliver the TA system to neurons.
Claim 45performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 46performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 47performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 48performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 49performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 50performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 51performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 52performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 53performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 54performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 55performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 56performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 57performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 58performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 59performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 60performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 61performancesupports2018Source 2needs review

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.
Claim 62performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 63performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 64performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 65performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 66performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 67performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 68performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 69performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 70performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 71performancesupports2018Source 2needs review

The TA system showed the best performance in HeLa, U-2 OS, and HEK-293 cells.

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.
Claim 72accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 73accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 74accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 75accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 76accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 77accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 78accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 79accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 80accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 81accessibilitysupports2016Source 1needs review

The Light Plate Apparatus can be built from components costing under $400 and can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 82accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 83accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 84accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 85accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 86accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 87accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 88accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 89accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 90accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 91accessibilitysupports2016Source 1needs review

The LPA components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.

All components can be purchased for under $400 and the device can be assembled and calibrated by a non-expert in one day.
assembly and calibration time one daycomponent cost 400 USD
Claim 92application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 93application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 94application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 95application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 96application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 97application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 98application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 99application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 100application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 101application scopesupports2016Source 1needs review

The Light Plate Apparatus simplifies entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 102application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 103application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 104application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 105application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 106application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 107application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 108application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 109application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 110application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 111application scopesupports2016Source 1needs review

The Light Plate Apparatus was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 112application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 113application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 114application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 115application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 116application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 117application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 118application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 119application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 120application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 121application scopesupports2016Source 1needs review

The LPA simplifies the entrainment of cyanobacterial circadian rhythm.

and simplify the entrainment of cyanobacterial circadian rhythm.
Claim 122application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 123application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 124application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 125application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 126application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 127application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 128application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 129application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 130application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 131application scopesupports2016Source 1needs review

The LPA was used to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells.

We use the LPA to precisely control gene expression from blue, green, and red light responsive optogenetic tools in bacteria, yeast, and mammalian cells
Claim 132capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 133capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 134capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 135capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 136capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 137capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 138capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 139capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 140capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 141capabilitysupports2016Source 1needs review

The Light Plate Apparatus can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 142engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 143engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 144engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 145engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 146engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 147engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 148engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 149engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 150engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 151engineering capabilitysupports2016Source 1needs review

The Light Plate Apparatus delivers two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.
independent light signals per well 2intensity control range three orders of magnitudeplate format 24-well platetemporal resolution millisecondwavelength range 310 to 1550 nm
Claim 152impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 153impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 154impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 155impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 156impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 157impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 158impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 159impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 160impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 161impactsupports2016Source 1needs review

The LPA reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 162impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 163impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 164impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 165impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 166impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 167impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 168impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 169impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 170impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 171impact statementsupports2016Source 1needs review

The Light Plate Apparatus reduces the entry barrier to optogenetics and photobiology experiments.

The LPA dramatically reduces the entry barrier to optogenetics and photobiology experiments.
Claim 172programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 173programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 174programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 175programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 176programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 177programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 178programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 179programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 180programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 181programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 182programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 183programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 184programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 185programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 186programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 187programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 188programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 189programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 190programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 191programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 192programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 193programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 194programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 195programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 196programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 197programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 198programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 199programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 200programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 201programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 202programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 203programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 204programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.
Claim 205programming interfacesupports2016Source 1needs review

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.

Approval Evidence

2 sources5 linked approval claimsfirst-pass slug iris
two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS)

Source:

Signals are programmed using an intuitive web tool named Iris.

Source:

application scopesupports

The study tested two BphP1-QPAS1-based optogenetic tools, iRIS and the TA system, in several mammalian cell types including cortical neurons.

Here, we tested the functionality of two BphP1-QPAS1-based optogenetic tools-an NIR- and blue-light-sensing system for control of protein localization (iRIS) and an NIR light-sensing system for transcription activation (TA)-in several cell types, including cortical neurons.

Source:

cell type dependencesupports

Performance of the BphP1-QPAS1-based optogenetic tools depended on physiological properties of specific cell types, such as nuclear transport, which could limit applicability of the blue-light-sensitive component of iRIS.

We found that the performance of these optogenetic tools often relied on physiological properties of a specific cell type, such as nuclear transport, which could limit the applicability of the blue-light-sensitive component of iRIS.

Source:

performancesupports

The NIR-light-sensing component of iRIS performed well in all tested cell types.

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.

Source:

programming interfacesupports

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.

Source:

programming interfacesupports

LPA signals are programmed using the web tool Iris.

Signals are programmed using an intuitive web tool named Iris.

Source:

Comparisons

Source-backed strengths

The main supported strength is that Iris is described as an intuitive web tool for programming signals. Its relevance is reinforced by use in studies involving BphP1-QPAS1-based optogenetic tools tested across several mammalian cell types, including cortical neurons.

Source:

In contrast, the NIR-light-sensing component of iRIS performed well in all tested cell types.

Source:

The TA system showed the best performance in cervical cancer (HeLa), bone cancer (U-2 OS), and human embryonic kidney (HEK-293) cells.

Source:

Here, we engineer the Light Plate Apparatus (LPA), a device that can deliver two independent 310 to 1550 nm light signals to each well of a 24-well plate with intensity control over three orders of magnitude and millisecond resolution.

Compared with LOVpep/ePDZb

Iris and LOVpep/ePDZb address a similar problem space because they share localization, transcription.

Shared frame: shared target processes: localization, transcription; same primary input modality: light

Strengths here: looks easier to implement in practice.

Compared with open-source microplate reader

Iris and open-source microplate reader address a similar problem space because they share transcription.

Shared frame: same top-level item type; shared target processes: transcription; same primary input modality: light

Strengths here: appears more independently replicated; looks easier to implement in practice.

Compared with optogenetic epigenetic editing toolbox for Ascl1 promoter targeting

Iris and optogenetic epigenetic editing toolbox for Ascl1 promoter targeting address a similar problem space because they share localization, transcription.

Shared frame: shared target processes: localization, transcription; same primary input modality: light

Strengths here: appears more independently replicated; looks easier to implement in practice.

Ranked Citations

  1. 1.
    StructuralSource 1Scientific Reports2016Claim 81Claim 80Claim 80

    Extracted from this source document.

  2. 2.
    StructuralSource 2ChemBioChem2018Claim 1Claim 15Claim 3

    Extracted from this source document.