Source 1primary paper2023MED
Toolkit/Cas12aVIP
Cas12aVIP
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Cas12aVIP is a rapid visual nucleic acid detection assay that integrates recombinase polymerase amplification, a CRISPR/Cas12a detection system, and a PMNT plus single-stranded DNA color reporter. It was proposed for visual readout of target nucleic acids in a format intended to be rapid and directly observable.
Usefulness & Problems
Why this is useful
This assay is useful because it combines nucleic acid amplification, CRISPR/Cas12a-based detection, and colorimetric reporting in a single method. The available evidence indicates that its purpose is rapid visual detection without relying solely on instrument-based signal acquisition.
Problem solved
Cas12aVIP addresses the problem of converting nucleic acid detection into a rapid visual assay format. The cited study specifically presents it as a method for nucleic acid detection of Escherichia coli O157:H7.
Problem links
enables naked-eye colorimetric DNA detection without ancillary equipment
LiteratureIt addresses the need for rapid, accurate, visual point-of-care testing methods for pathogenic bacteria detection. The method is positioned as useful for avoiding foodborne diseases caused by pathogens or their toxins.
Source:
It addresses the need for rapid, accurate, visual point-of-care testing methods for pathogenic bacteria detection. The method is positioned as useful for avoiding foodborne diseases caused by pathogens or their toxins.
supports rapid detection of Escherichia coli O157:H7
LiteratureIt addresses the need for rapid, accurate, visual point-of-care testing methods for pathogenic bacteria detection. The method is positioned as useful for avoiding foodborne diseases caused by pathogens or their toxins.
Source:
It addresses the need for rapid, accurate, visual point-of-care testing methods for pathogenic bacteria detection. The method is positioned as useful for avoiding foodborne diseases caused by pathogens or their toxins.
Published Workflows
Objective: Develop a rapid, accurate, visual point-of-care nucleic acid detection method for Escherichia coli O157:H7 using a CRISPR/Cas12a-PMNT assay format.
Why it works: The workflow combines nucleic acid amplification with CRISPR/Cas12a detection and a PMNT/ssDNA color reporter so that target presence is converted into a visible red/yellow color change observable by eye.
target-DNA-triggered CRISPR/Cas12a detectionPMNT conformational color change for colorimetric readoutrecombinase polymerase amplificationCRISPR/Cas12a assaycolorimetric reporting
Stages
- 1.Target nucleic acid amplification(functional_characterization)
RPA is included as an assay component in the combined method to support downstream target detection.
Selection: Amplify target nucleic acid using recombinase polymerase amplification before CRISPR/Cas12a and colorimetric readout.
- 2.CRISPR/Cas12a target detection with PMNT color transduction(confirmatory_validation)
This stage provides the assay's visual colorimetric readout under natural light.
Selection: Use the CRISPR/Cas12a system together with PMNT mixed with ssDNA to convert target presence into a red/yellow colorimetric output.
- 3.Specificity assessment against nontargeted bacteria(secondary_characterization)
This stage checks that the visual detection method remains specific for the intended target.
Selection: Evaluate whether the method shows high specificity and no interference from other nontargeted bacteria.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
colorimetric visual reporting via pmnt and single-stranded dna reportercrispr/cas12a-mediated nucleic acid detectionrecombinase polymerase amplificationTechniques
Functional AssayTarget processes
editingrecombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The assay uses recombinase polymerase amplification together with a CRISPR/Cas12a system and a PMNT mixed with a single-stranded DNA color reporter. The supplied evidence does not specify construct sequences, reaction conditions, Cas12a source, or sample preparation requirements.
The supplied evidence is limited to a single study and brief design-level claims. No independent replication, analytical sensitivity, specificity, false-positive behavior, or cross-matrix validation details are provided in the evidence set.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug cas12avip
In this study, we proposed a rapid and visual detection method that we named "Cas12aVIP".
Source:
mechanism or designsupports
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Source:
method introductionsupports
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
Source:
performance timesupports
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
Source:
specificitysupports
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Source:
usabilitysupports
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Source:
Comparisons
Source-stated alternatives
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Source:
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Source-backed strengths
The reported design integrates amplification, CRISPR-based recognition, and a PMNT/ssDNA visual reporter, which supports a compact assay workflow. The evidence explicitly describes the method as rapid and visual, but provides limited quantitative performance detail in the supplied record.
Source:
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
Compared with assays
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Shared frame: source-stated alternative in extracted literature
Strengths here: high specificity; no interference from other nontargeted bacteria; 40 min detection time.
Source:
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Compared with CRISPR/Cas9
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Shared frame: source-stated alternative in extracted literature
Strengths here: high specificity; no interference from other nontargeted bacteria; 40 min detection time.
Source:
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Compared with CRISPR/Cas9 system
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Shared frame: source-stated alternative in extracted literature
Strengths here: high specificity; no interference from other nontargeted bacteria; 40 min detection time.
Source:
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Compared with DETECTR
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Shared frame: source-stated alternative in extracted literature
Strengths here: high specificity; no interference from other nontargeted bacteria; 40 min detection time.
Source:
The upstream summary identifies related Cas12a diagnostic formats such as DETECTR, RAA-based Cas12a assays, and LAMP-CRISPR/Cas12a configurations as nearby alternatives with different amplification or readout choices.
Ranked Citations
- 1.