Source 1primary paper2023MED
Toolkit/Cas12aVIP
Cas12aVIP
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Cas12aVIP is a rapid visual nucleic acid detection assay that integrates recombinase polymerase amplification, a CRISPR/Cas12a detection system, and a PMNT plus single-stranded DNA color reporter. It was proposed for visual readout of target nucleic acids in a format intended to be rapid and directly observable.
Usefulness & Problems
Why this is useful
This assay is useful because it combines nucleic acid amplification, CRISPR/Cas12a-based detection, and colorimetric reporting in a single method. The available evidence indicates that its purpose is rapid visual detection without relying solely on instrument-based signal acquisition.
Problem solved
Cas12aVIP addresses the problem of converting nucleic acid detection into a rapid visual assay format. The cited study specifically presents it as a method for nucleic acid detection of Escherichia coli O157:H7.
Problem links
This item is explicitly described as a rapid and visual detection method, which directly aligns with the gap's need for earlier pathogen detection. A CRISPR-based assay could plausibly support surveillance workflows if adapted to relevant threat nucleic acid targets.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
colorimetric visual reporting via pmnt and single-stranded dna reportercolorimetric visual reporting via pmnt and single-stranded dna reportercrispr/cas12a-mediated nucleic acid detectioncrispr/cas12a-mediated nucleic acid detectionrecombinase polymerase amplificationrecombinase polymerase amplificationTechniques
Functional AssayTarget processes
editingrecombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknowndetection time min: 40encoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensorpoint of care positioning: Truevisual readout: True
The assay uses recombinase polymerase amplification together with a CRISPR/Cas12a system and a PMNT mixed with a single-stranded DNA color reporter. The supplied evidence does not specify construct sequences, reaction conditions, Cas12a source, or sample preparation requirements.
The supplied evidence is limited to a single study and brief design-level claims. No independent replication, analytical sensitivity, specificity, false-positive behavior, or cross-matrix validation details are provided in the evidence set.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
detection time 40 min
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
In the reported PMNT/ssDNA reporter system, the solution is red in the absence of target DNA and yellow when target DNA is detected, enabling colorimetric DNA detection.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Approval Evidence
1 source5 linked approval claimsfirst-pass slug cas12avip
In this study, we proposed a rapid and visual detection method that we named "Cas12aVIP".
Source:
mechanism or designsupports
Cas12aVIP combines recombinase polymerase amplification, a CRISPR/Cas12a system, and a PMNT mixed with single-stranded DNA color reporter.
Source:
method introductionsupports
The paper proposes Cas12aVIP as a rapid and visual nucleic acid detection method.
Source:
performance timesupports
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
Source:
specificitysupports
Cas12aVIP yielded high specificity with no interference from other nontargeted bacteria.
Source:
usabilitysupports
Cas12aVIP has potential for rapid nucleic acid detection applications without requiring technical expertise or ancillary equipment.
Source:
Comparisons
Source-backed strengths
The reported design integrates amplification, CRISPR-based recognition, and a PMNT/ssDNA visual reporter, which supports a compact assay workflow. The evidence explicitly describes the method as rapid and visual, but provides limited quantitative performance detail in the supplied record.
Source:
Detection with Cas12aVIP was accomplished in 40 minutes and could be observed by the naked eye under natural light.
Compared with droplet microfluidic platform
Cas12aVIP and droplet microfluidic platform address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Compared with fluorescence recovery after photobleaching
Cas12aVIP and fluorescence recovery after photobleaching address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Compared with open-source microplate reader
Cas12aVIP and open-source microplate reader address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Ranked Citations
- 1.