Laue diffraction

Stages

1. 1.
   Reaction initiation in crystal(selection)

   This stage creates the transient structural species that kinetic crystallography aims to observe.

   Selection: Initiate biological turnover in the crystal so transient structural species form.

2. 2.
   Time-resolved observation by Laue diffraction(functional_characterization)

   This stage films transient structural species on the fly in reaction regimes best suited to Laue diffraction.

   Selection: Use Laue diffraction when reactions are cyclic, ultra-fast, or light-triggered.

3. 3.
   Intermediate capture by trapping methods(functional_characterization)

   This stage captures transient species in systems where Laue diffraction is less suitable.

   Selection: Use trapping approaches for a wider range of biological systems.

4. 4.
   Spectroscopic design, interpretation, and validation(confirmatory_validation)

   Complementary spectroscopy is described as essential for designing, interpreting, and validating kinetic crystallography experiments.

   Selection: Apply complementary methods, mainly UV/visible single-crystal spectroscopy, to support experiment design, interpretation, and validation.

Steps

1. 1.
   Initiate biological turnover in the crystaloverall method

   Generate transient structural species inside the crystal.

   Transient species must first be formed before they can be observed by diffraction or captured by trapping.

2. 2.
   Film transient structural species by Laue diffractionstructural readout method

   Observe transient structural species on the fly.

   After turnover initiation creates transient species, Laue diffraction can capture them in real time when the reaction regime is suitable.

3. 3.
   Capture transient species by trapping methodsalternative structural capture method

   Capture transient structural species in systems less suited to Laue diffraction.

   Trapping is used after turnover initiation when the goal is to study a wider range of biological systems, but with attention to artefact risk.

4. 4.
   Use complementary single-crystal spectroscopy to design, interpret, and validate the experimentcomplementary validation method

   Support experiment design, interpretation, and validation with spectroscopic evidence.

   Complementary spectroscopy is described as essential for validating and interpreting kinetic crystallography results and for designing the experiments appropriately.