Source 1primary paper2020Physical Review Letters
Toolkit/leaflet-specific differently colored dye reporting of lipid registration
leaflet-specific differently colored dye reporting of lipid registration
Also known as: differently colored dyes as reporters
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bilayer.
Usefulness & Problems
Why this is useful
This reporter strategy uses differently colored dyes to track registration of ordered and disordered lipids across both membrane leaflets.; reporting transbilayer registration of ordered and disordered lipids; observing leaflet-resolved membrane domain alignment
Source:
This reporter strategy uses differently colored dyes to track registration of ordered and disordered lipids across both membrane leaflets.
Source:
reporting transbilayer registration of ordered and disordered lipids
Source:
observing leaflet-resolved membrane domain alignment
Problem solved
It addresses the need to observe whether ordered and disordered domains are registered between the two leaves of a bilayer.; provides optical reporting of lipid registration across the two membrane leaflets
Source:
It addresses the need to observe whether ordered and disordered domains are registered between the two leaves of a bilayer.
Source:
provides optical reporting of lipid registration across the two membrane leaflets
Problem links
provides optical reporting of lipid registration across the two membrane leaflets
LiteratureIt addresses the need to observe whether ordered and disordered domains are registered between the two leaves of a bilayer.
Source:
It addresses the need to observe whether ordered and disordered domains are registered between the two leaves of a bilayer.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensorswitch architecture: recruitment
It requires a freestanding bilayer and differently colored leaflet reporters; the abstract does not provide dye names or loading details.; requires differently colored dyes assigned to the two leaves of a freestanding bilayer
The abstract does not establish that the dyes alone directly measure nanoscale domain size without additional analysis.; the abstract does not specify dye identities, calibration, or resolution limits
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Photoswitchable lipids dissolved or reformed membrane domains.
Photoswitchable lipids dissolved or reformed the domains.
Differently colored dyes were used as reporters for registration of ordered and disordered lipids from both leaflets of a freestanding bilayer.
Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bilayer.
Domain mobility measurements indicated ordered domains of about 120 nm width and disordered domains of about 40 nm width.
Measurements of domain mobility indicated the presence of 120 nm wide ordered and 40 nm wide disordered domains.
disordered domain width 40 nmordered domain width 120 nm
The inferred domain sizes are consistent with predicted roles of line tension and membrane undulation as driving forces for alignment.
These sizes are in line with the predicted roles of line tension and membrane undulation as driving forces for alignment.
Approval Evidence
1 source1 linked approval claimfirst-pass slug leaflet-specific-differently-colored-dye-reporting-of-lipid-registration
Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bilayer.
Source:
measurement resultsupports
Differently colored dyes were used as reporters for registration of ordered and disordered lipids from both leaflets of a freestanding bilayer.
Here we used differently colored dyes as reporters for the registration of both ordered and disordered lipids from the two leaves of a freestanding bilayer.
Source:
Comparisons
Source-backed strengths
distinguishes signals from the two leaves of a bilayer using different colors
Source:
distinguishes signals from the two leaves of a bilayer using different colors
Compared with Langendorff perfused heart electrical recordings
leaflet-specific differently colored dye reporting of lipid registration and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with native green gel system
leaflet-specific differently colored dye reporting of lipid registration and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
leaflet-specific differently colored dye reporting of lipid registration and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.