Source 1primary paper2019Biomolecules
Toolkit/light-induced co-clustering assay
light-induced co-clustering assay
Also known as: light-induced co-clustering assays
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The light-induced co-clustering assay is an optogenetic functional assay used to assess protein-protein interactions in Drosophila S2 cells. It was reported in the context of light-induced protein clustering and uses co-clustering as the interaction readout.
Usefulness & Problems
Why this is useful
This assay is useful for testing whether proteins associate in living Drosophila S2 cells using a light-controlled readout. The available evidence supports its use as a cell-based interaction assay, but does not provide broader performance comparisons or application scope.
Source:
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Problem solved
It addresses the need to assess protein-protein interactions in S2 cells with a light-induced co-clustering readout. The supplied evidence does not specify whether it improves on particular conventional interaction assays.
Source:
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Problem links
Need precise spatiotemporal control with light input
DerivedThe light-induced co-clustering assay is an optogenetic assay method validated for assessing protein-protein interactions in Drosophila S2 cells. It uses light as the input modality and was reported in the context of light-induced protein clustering.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
co-clustering-based protein interaction readoutco-clustering-based protein interaction readoutlight-induced protein clusteringlight-induced protein clusteringTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The reported implementation context is Drosophila S2 cells and a light input modality. The available evidence does not specify the photoreceptor system, illumination wavelength, construct architecture, or expression and imaging conditions.
The supplied evidence is limited to a single statement of validation in S2 cells. No quantitative performance metrics, target classes, temporal properties, false-positive behavior, or validation in other organisms or cell types are provided.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Approval Evidence
1 source1 linked approval claimfirst-pass slug light-induced-co-clustering-assay
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Source:
applicationsupports
Light-induced co-clustering assays were validated to assess protein-protein interactions in Drosophila S2 cells.
Moreover, we validated light-induced co-clustering assays to assess protein-protein interactions in S2 cells.
Source:
Comparisons
Source-backed strengths
The assay was explicitly validated for protein-protein interaction assessment in Drosophila S2 cells. Its readout is linked to light-induced protein clustering, providing an optogenetic and cell-based format.
Compared with native green gel system
light-induced co-clustering assay and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with open-source microplate reader
light-induced co-clustering assay and open-source microplate reader address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with plant transcriptome profiling
light-induced co-clustering assay and plant transcriptome profiling address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.