Source 1primary paper2015ACS Synthetic Biology
Toolkit/LOV-PTS1
LOV-PTS1
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
LOV-PTS1 is a light-responsive protein tag that is appended to proteins of interest to direct their import into the peroxisome. It uses the AsLOV2 photosensory domain to photocage a peroxisomal targeting sequence and thereby enable light-regulated peroxisomal trafficking.
Usefulness & Problems
Why this is useful
This construct enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times. It is therefore useful for experiments requiring optical control over the subcellular localization of a tagged protein.
Source:
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
Source:
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
Problem solved
LOV-PTS1 addresses the problem of controlling peroxisomal protein import with temporal and cell-specific precision. The reported design converts peroxisomal targeting into a light-regulated process rather than a constitutive localization signal.
Source:
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
Problem links
Need precise spatiotemporal control with light input
DerivedLOV-PTS1 is a light-responsive protein tag that is appended to proteins of interest to direct their import into the peroxisome. It uses the AsLOV2 photosensory domain to photocage a peroxisomal targeting sequence and thereby enable light-regulated peroxisomal trafficking.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The construct design uses the AsLOV2 photosensory domain fused in a way that photocages a peroxisomal targeting sequence (PTS1). The available evidence supports use as an appended tag on proteins of interest, but it does not provide additional practical details on illumination parameters, expression systems, or delivery methods.
The supplied evidence does not report quantitative performance metrics such as import kinetics, dynamic range, reversibility, or background import in the dark. Evidence is also limited to the originating publication, so independent replication and breadth across organisms or cargo classes are not established here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
This method enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times.
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
This method enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times.
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
This method enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times.
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
This method enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times.
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
This method enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times.
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
This method enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times.
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
This method enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times.
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
AsLOV2 was used to photocage a peroxisomal targeting sequence, enabling light regulation of peroxisomal protein import.
Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import.
LOV-PTS1 is a protein tag that can be appended to proteins of interest to direct their import to the peroxisome with light.
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
LOV-PTS1 is a protein tag that can be appended to proteins of interest to direct their import to the peroxisome with light.
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
LOV-PTS1 is a protein tag that can be appended to proteins of interest to direct their import to the peroxisome with light.
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
LOV-PTS1 is a protein tag that can be appended to proteins of interest to direct their import to the peroxisome with light.
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
LOV-PTS1 is a protein tag that can be appended to proteins of interest to direct their import to the peroxisome with light.
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
LOV-PTS1 is a protein tag that can be appended to proteins of interest to direct their import to the peroxisome with light.
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
LOV-PTS1 is a protein tag that can be appended to proteins of interest to direct their import to the peroxisome with light.
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug lov-pts1
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
Source:
application scopesupports
This method enables inducible triggering of peroxisomal protein trafficking in specific cells at user-defined times.
This method provides a means to inducibly trigger peroxisomal protein trafficking in specific cells at user-defined times.
Source:
tool functionsupports
LOV-PTS1 is a protein tag that can be appended to proteins of interest to direct their import to the peroxisome with light.
We generated a protein tag, LOV-PTS1, that can be appended to proteins of interest to direct their import to the peroxisome with light.
Source:
Comparisons
Source-backed strengths
The tool is described as a modular protein tag that can be appended to proteins of interest, which supports straightforward retargeting of different cargo proteins. Its key validated feature is light-dependent direction of protein import to the peroxisome through AsLOV2-based photocaging of a peroxisomal targeting sequence.
Source:
Here, we used AsLOV2 to photocage a peroxisomal targeting sequence, allowing light regulation of peroxisomal protein import.
Compared with optogenetic probes
LOV-PTS1 and optogenetic probes address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with opto-PROTAC
LOV-PTS1 and opto-PROTAC address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocaging; same primary input modality: light
Compared with pc-PROTAC1
LOV-PTS1 and pc-PROTAC1 address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: photocaging; same primary input modality: light
Ranked Citations
- 1.