LOV-PTS1 is a light-responsive protein tag that is appended to proteins of interest to direct their import into the peroxisome. It uses the AsLOV2 photosensory domain to photocage a peroxisomal targeting sequence and thereby enable light-regulated peroxisomal trafficking.
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Mechanism Concept
photocaging
A mechanism-level grouping derived from the current toolkit evidence. Current coverage includes 4 architectures and 2 components. Across those tools, the main enabled capabilities are degradation, editing, and recombination. Representative components include bow-knot-type gRNA and cyanine photocages.
6 total items4 architectures2 components
Main enabled capabilities: degradation, editing, recombination.
Architectures
4 of 4opto-PROTAC is a light-inducible PROTAC design in which a photolabile caging group is installed on pomalidomide-based degraders to block activity in the dark and permit target protein degradation after ultraviolet A irradiation. It was demonstrated using caged pomalidomide and the PROTACs dBET1 and dALK to achieve spatiotemporal control of protein destruction.
pc-PROTAC1 is a photocaged PROTAC construct designed for light-dependent targeted protein degradation in live cells. In the cited study, it exhibited potent degradation activity only after light irradiation, establishing a light-activated degradation strategy.
we developed photoactivatable naloxone (PhNX)
Components
2 of 2The bow-knot-type gRNA is a light-responsive guide RNA format for CRISPR-Cas editing developed within a cyclically caged gRNA strategy. It is reported to enable optical control of gene editing, including light-mediated MSTN editing in embryos, while showing no background editing without light irradiation.
The review focuses on phototherapeutics responsive in the 600–900 nm optical window and explicitly highlights cyanine-based near-IR photocages.