Source 1primary paper2019Journal of the American Chemical Society
Toolkit/pc-PROTAC1
pc-PROTAC1
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
pc-PROTAC1 is a photocaged PROTAC construct designed for light-dependent targeted protein degradation in live cells. In the cited study, it exhibited potent degradation activity only after light irradiation, establishing a light-activated degradation strategy.
Usefulness & Problems
Why this is useful
pc-PROTAC1 is useful as a chemical tool for imposing optical control over PROTAC-mediated protein degradation in live cells. The cited work supports its value for experiments requiring degradation activity to be switched on by light rather than being constitutively active.
Source:
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Problem solved
pc-PROTAC1 addresses the problem of how to trigger PROTAC-induced protein degradation conditionally with light. The cited study presents it as part of a general strategy for inducing protein degradation with light.
Problem links
Need conditional protein clearance
Derivedpc-PROTAC1 is a photocaged PROTAC construct that enables light-dependent protein degradation in live cells. In the cited study, pc-PROTAC1 showed potent degradation activity only after light irradiation.
Need precise spatiotemporal control with light input
Derivedpc-PROTAC1 is a photocaged PROTAC construct that enables light-dependent protein degradation in live cells. In the cited study, pc-PROTAC1 showed potent degradation activity only after light irradiation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
Degradationlight-triggered activationlight-triggered activationphotocagingphotocagingtargeted protein degradationtargeted protein degradationTechniques
No technique tags yet.
Target processes
degradationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuatoroperating role: regulator
Implementation requires a photocaged PROTAC construct and light irradiation to activate degradation in live cells. The available evidence does not report construct composition, delivery conditions, illumination parameters, or other practical protocol details.
The supplied evidence does not specify the degraded target protein, photocaging group, irradiation wavelength, kinetics, reversibility, or quantitative degradation efficiency. Independent replication and validation beyond the cited study are not provided in the available evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
Photo-caged PROTACs can induce protein degradation activity with light.
Here we present a type of photo-caged PROTACs (pc-PROTACs) to induce degradation activity with light.
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
The photocaged PROTAC approach was successfully applied to construct pc-PROTAC3 of BTK.
this approach was successfully applied to construct pc-PROTAC3 of BTK
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
A general strategy to induce protein degradation with light was established.
Thus, a general strategy to induce protein degradation with light was established
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
Approval Evidence
1 source2 linked approval claimsfirst-pass slug pc-protac1
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
Source:
conditional activitysupports
pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation.
the resulting molecule pc-PROTAC1 showed potent degradation activity in live cells only after light irradiation
Source:
targeted degradationsupports
pc-PROTAC1 efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish.
this molecule efficiently degraded Brd4 and induced expected phenotypic changes in zebrafish
Source:
Comparisons
Source-backed strengths
The key demonstrated strength is potent degradation activity in live cells after light irradiation. The evidence also supports that the inactive-to-active transition is light dependent, consistent with successful photocaging of PROTAC function.
Compared with GFP-CRY2
pc-PROTAC1 and GFP-CRY2 address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation; same primary input modality: light
Compared with photo-caged PROTACs
pc-PROTAC1 and photo-caged PROTACs address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation, light-triggered activation, targeted protein degradation; same primary input modality: light
Compared with TRIM21-nanobody chimeras
pc-PROTAC1 and TRIM21-nanobody chimeras address a similar problem space because they share degradation.
Shared frame: same top-level item type; shared target processes: degradation; shared mechanisms: degradation, targeted protein degradation; same primary input modality: light
Ranked Citations
- 1.