Source 1primary paper2014The Journal of Physical Chemistry B
Toolkit/magnetic-field dependent 13C NMR spectroscopy
magnetic-field dependent 13C NMR spectroscopy
Also known as: photo-CIDNP studies at four different magnetic fields, solution 13C NMR spectra
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Magnetic-field dependent 13C NMR spectroscopy is a solution-state photo-CIDNP assay in which 13C NMR spectra are acquired across multiple magnetic fields under light activation. In the cited 2014 study, it was applied to the LOV2-C450A domain of phototropin over 4.7–11.8 T to detect field-dependent 13C photo-CIDNP signals and support interpretation involving a novel triplet mechanism.
Usefulness & Problems
Why this is useful
This assay is useful for probing light-driven spin-dependent polarization effects in flavoproteins by comparing 13C photo-CIDNP behavior across magnetic field strengths. The source states that the identified triplet mechanism opens new means of studying the electronic structures of active cofactors in flavoproteins at atomic resolution.
Problem solved
It addresses the problem of mechanistically interpreting photo-CIDNP signals in a protein system by using magnetic-field dependence as a discriminating variable. In the reported application, field-dependent solution 13C NMR data from LOV2-C450A were used to interpret the observed photo-CIDNP in terms of solid-state mechanisms, including a novel triplet mechanism.
Problem links
Need precise spatiotemporal control with light input
DerivedMagnetic-field dependent 13C NMR spectroscopy, as described here, is a photo-CIDNP assay performed in solution 13C NMR across magnetic fields from 4.7 to 11.8 T. In the cited study, it was applied to the LOV2-C450A domain of phototropin to detect and interpret field-dependent 13C photo-CIDNP signals, including evidence for a novel triplet mechanism.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
photochemically induced dynamic nuclear polarization (photo-cidnp)photochemically induced dynamic nuclear polarization (photo-cidnp)triplet mechanismtriplet mechanismTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The reported implementation used solution 13C NMR with photo-CIDNP measurements under light input at four magnetic fields spanning 4.7 to 11.8 T. The demonstrated biological system was the LOV2-C450A domain of phototropin, but the supplied evidence does not provide further construct, cofactor, or sample preparation details.
The evidence is limited to a single cited study in the LOV2-C450A domain of phototropin, so generality beyond this flavoprotein context is not established here. Practical performance characteristics such as sensitivity, throughput, sample requirements, and robustness across other targets are not described in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
Approval Evidence
1 source3 linked approval claimsfirst-pass slug magnetic-field-dependent-13c-nmr-spectroscopy
photo-CIDNP observed in solution 13C NMR spectra ... photo-CIDNP studies were performed at four different magnetic fields ranging from 4.7 to 11.8 T
Source:
field dependent agreementsupports
At high magnetic fields, the calculated enhancement factors of 3F agree favorably with experimental counterparts.
whereas at high magnetic fields, the calculated enhancement factors of 3F agree favorably with their experimental counterparts
Source:
mechanistic interpretationsupports
Photo-CIDNP observed in solution 13C NMR spectra of LOV2-C450A can be reliably interpreted in terms of solid-state mechanisms including a novel triplet mechanism.
demonstrate that photo-CIDNP observed in solution 13C NMR spectra can reliably be interpreted in terms of solid-state mechanisms including a novel triplet mechanism
Source:
method potentialsupports
The novel triplet mechanism opens new means of studying electronic structures of active cofactors in flavoproteins at atomic resolution.
the novel triplet mechanism opens up new means of studying electronic structures of the active cofactors in these proteins at atomic resolution
Source:
Comparisons
Source-backed strengths
The method provided experimentally observable field-dependent 13C photo-CIDNP data across four magnetic fields from 4.7 to 11.8 T. At high magnetic fields, calculated enhancement factors of 3F agreed favorably with experimental values, supporting the mechanistic interpretation in the studied system.
Compared with native green gel system
magnetic-field dependent 13C NMR spectroscopy and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with open-source microplate reader
magnetic-field dependent 13C NMR spectroscopy and open-source microplate reader address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with plant transcriptome profiling
magnetic-field dependent 13C NMR spectroscopy and plant transcriptome profiling address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.