Source 1review2019Chemical Reviews
Toolkit/magnetic tweezers
magnetic tweezers
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we review a broad spectrum of single-molecule tools and techniques such as optical and magnetic tweezers...
Usefulness & Problems
Why this is useful
Magnetic tweezers are described as a single-molecule technique for manipulating biomolecules and monitoring DNA motor behavior. The review includes them among the main tools used to study mechanochemical coupling.; single-molecule manipulation of biomolecules; applying force and torque to individual motor complexes; observing motor dynamics
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Magnetic tweezers are described as a single-molecule technique for manipulating biomolecules and monitoring DNA motor behavior. The review includes them among the main tools used to study mechanochemical coupling.
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single-molecule manipulation of biomolecules
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applying force and torque to individual motor complexes
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observing motor dynamics
Problem solved
It provides a way to probe motor reaction cycles and conformational dynamics one molecule at a time.; enables direct manipulation and observation of individual biomolecules during motor activity
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It provides a way to probe motor reaction cycles and conformational dynamics one molecule at a time.
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enables direct manipulation and observation of individual biomolecules during motor activity
Problem links
Magnetic tweezers are a single-molecule structural/functional characterization method and could plausibly probe dynamic conformational landscapes or force-dependent allosteric behavior. That makes them potentially relevant for understanding proteins whose structures fluctuate continuously.
enables direct manipulation and observation of individual biomolecules during motor activity
LiteratureIt provides a way to probe motor reaction cycles and conformational dynamics one molecule at a time.
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It provides a way to probe motor reaction cycles and conformational dynamics one molecule at a time.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
Conformational Uncagingmechanical force applicationsingle-molecule manipulationtorque applicationTarget processes
No target processes tagged yet.
Input: Magnetic
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensorswitch architecture: uncaging
The supplied abstract supports a setup that can apply forces and torques to individual biomolecules. No further implementation detail is given here.; requires single-molecule experimental setup capable of force and torque manipulation
The abstract does not specify unique limitations or claim that magnetic tweezers alone capture every relevant observable.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The reviewed single-molecule methods enable manipulation of individual biomolecules through applied forces and torques and observation of dynamic conformational changes in single motor complexes.
Mutagenesis, chemical modifications, and optogenetics have been used to re-engineer existing molecular motors to alter speed, processivity, or functionality.
In-depth analysis of mechanochemical coupling in molecular motors has enabled development of artificially engineered motors.
Single-molecule techniques have been extensively used to identify structural intermediates in molecular motor reaction cycles and to understand how substeps in energy consumption drive transitions between intermediates.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug magnetic-tweezers
Here, we review a broad spectrum of single-molecule tools and techniques such as optical and magnetic tweezers...
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capability summarysupports
The reviewed single-molecule methods enable manipulation of individual biomolecules through applied forces and torques and observation of dynamic conformational changes in single motor complexes.
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review scope summarysupports
Single-molecule techniques have been extensively used to identify structural intermediates in molecular motor reaction cycles and to understand how substeps in energy consumption drive transitions between intermediates.
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Comparisons
Source-stated alternatives
Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
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Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Source-backed strengths
supports application of forces and torques; enables observation of dynamic conformational changes in single motor complexes
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supports application of forces and torques
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enables observation of dynamic conformational changes in single motor complexes
Compared with nanopore tweezers
Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports application of forces and torques; enables observation of dynamic conformational changes in single motor complexes.
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Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Compared with optical tweezers
Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports application of forces and torques; enables observation of dynamic conformational changes in single motor complexes.
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Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Compared with single-molecule fluorescence resonance energy transfer
Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports application of forces and torques; enables observation of dynamic conformational changes in single motor complexes.
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Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Compared with single-molecule FRET
Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Shared frame: source-stated alternative in extracted literature
Strengths here: supports application of forces and torques; enables observation of dynamic conformational changes in single motor complexes.
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Named alternatives in the abstract include optical tweezers, AFM, smFRET, nanopore tweezers, and hybrid techniques.
Ranked Citations
- 1.