Source 1review2019Chemical Reviews
Toolkit/nanopore tweezers
nanopore tweezers
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Here, we review a broad spectrum of single-molecule tools and techniques such as ... nanopore tweezers...
Usefulness & Problems
Why this is useful
Nanopore tweezers are named as part of the review's single-molecule toolkit for DNA motor analysis. The abstract supports their use in studying individual biomolecules and motor dynamics.; single-molecule analysis of DNA motors; observing motor dynamics
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Nanopore tweezers are named as part of the review's single-molecule toolkit for DNA motor analysis. The abstract supports their use in studying individual biomolecules and motor dynamics.
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single-molecule analysis of DNA motors
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observing motor dynamics
Problem solved
It contributes to single-molecule observation of motor behavior and mechanochemical processes.; provides a single-molecule method for studying motor-associated dynamics
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It contributes to single-molecule observation of motor behavior and mechanochemical processes.
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provides a single-molecule method for studying motor-associated dynamics
Problem links
provides a single-molecule method for studying motor-associated dynamics
LiteratureIt contributes to single-molecule observation of motor behavior and mechanochemical processes.
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It contributes to single-molecule observation of motor behavior and mechanochemical processes.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensorswitch architecture: uncaging
The supplied text supports a nanopore-based single-molecule setup, but does not provide further implementation details.; requires nanopore-based single-molecule measurement setup
The abstract does not specify its limitations or comparative weaknesses.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The reviewed single-molecule methods enable manipulation of individual biomolecules through applied forces and torques and observation of dynamic conformational changes in single motor complexes.
Mutagenesis, chemical modifications, and optogenetics have been used to re-engineer existing molecular motors to alter speed, processivity, or functionality.
In-depth analysis of mechanochemical coupling in molecular motors has enabled development of artificially engineered motors.
Single-molecule techniques have been extensively used to identify structural intermediates in molecular motor reaction cycles and to understand how substeps in energy consumption drive transitions between intermediates.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug nanopore-tweezers
Here, we review a broad spectrum of single-molecule tools and techniques such as ... nanopore tweezers...
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capability summarysupports
The reviewed single-molecule methods enable manipulation of individual biomolecules through applied forces and torques and observation of dynamic conformational changes in single motor complexes.
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review scope summarysupports
Single-molecule techniques have been extensively used to identify structural intermediates in molecular motor reaction cycles and to understand how substeps in energy consumption drive transitions between intermediates.
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Comparisons
Source-stated alternatives
The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
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The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Source-backed strengths
included among methods that enable manipulation or observation of individual biomolecules
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included among methods that enable manipulation or observation of individual biomolecules
Compared with magnetic tweezers
The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Shared frame: source-stated alternative in extracted literature
Strengths here: included among methods that enable manipulation or observation of individual biomolecules.
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The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Compared with optical tweezers
The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Shared frame: source-stated alternative in extracted literature
Strengths here: included among methods that enable manipulation or observation of individual biomolecules.
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The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Compared with single-molecule fluorescence resonance energy transfer
The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Shared frame: source-stated alternative in extracted literature
Strengths here: included among methods that enable manipulation or observation of individual biomolecules.
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The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Compared with single-molecule FRET
The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Shared frame: source-stated alternative in extracted literature
Strengths here: included among methods that enable manipulation or observation of individual biomolecules.
Source:
The abstract lists optical tweezers, magnetic tweezers, AFM, smFRET, and hybrid techniques as nearby alternatives or complements.
Ranked Citations
- 1.