Source 1primary paper2023
Toolkit/Material-to-cell synNotch ligand platforms
Material-to-cell synNotch ligand platforms
Also known as: material-to-cell signaling pathways, suite of materials to activate synNotch receptors
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Material-to-cell synNotch ligand platforms are engineered biomaterial and extracellular matrix systems that present synNotch ligands to mammalian cells. In the reported 2023 implementation, ligands were covalently incorporated into gelatin hydrogels or into cell-generated fibronectin-containing extracellular matrix to activate synthetic Notch receptors and induce programmed transcriptional outputs.
Usefulness & Problems
Why this is useful
These platforms provide a generalizable way to create user-defined material-to-cell signaling pathways using synNotch receptors. They are useful for spatially controlling cell phenotypes within multicellular constructs, including patterned transdifferentiation relevant to tissue engineering.
Source:
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
Source:
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
Source:
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
Source:
Synthetic Notch (synNotch) receptors are modular synthetic components that are genetically engineered into mammalian cells to detect signals presented by neighboring cells and respond by activating prescribed transcriptional programs.
Problem solved
The platform addresses the problem of delivering synNotch-activating ligands from noncellular or matrix-associated materials rather than from neighboring engineered sender cells. This enables prescribed spatial signaling cues in hydrogels or extracellular matrix for controlling cell-state transitions in designed tissue constructs.
Source:
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
Source:
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
Source:
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
Problem links
Need conditional control of signaling activity
DerivedMaterial-to-cell synNotch ligand platforms are engineered material systems that present synNotch ligands to mammalian cells, enabling user-defined material-to-cell signaling pathways. In the reported implementation, ligands were incorporated into hydrogels or cell-generated extracellular matrix to activate synthetic Notch receptors and drive prescribed transcriptional programs.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A delivery strategy grouped with the mechanism branch because it determines how a system is instantiated and deployed in context.
Mechanisms
covalent ligand immobilizationcovalent ligand immobilizationmaterial-presented ligand recognitionmaterial-presented ligand recognitionsynthetic notch receptor activationsynthetic receptor activationtranscriptional program inductiontranscriptional program inductionTechniques
No technique tags yet.
Target processes
signalingInput: Chemical
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: externally suppliedimplementation constraint: context specific validationoperating role: delivery
Reported implementations used covalent ligand immobilization on gelatin polymers by enzymatic chemistry or click chemistry, and synNotch ligand display within cell-generated extracellular matrix by genetically engineering fibroblast-produced fibronectin. The system depends on mammalian cells expressing compatible synNotch receptors and on construct designs that position immobilized ligands in hydrogels or matrix environments where cells are cultured on or within the material.
The evidence is currently limited to a single 2023 study and the supplied record does not report independent replication. The available evidence does not quantify activation strength, kinetics, long-term stability, or generality across multiple ligand-receptor pairs, material classes, or in vivo settings.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands can be covalently linked to gelatin polymers by enzymatic or click chemistry to activate synNotch receptors in cells grown on or within a hydrogel.
we then used enzymatic or click chemistry to covalently link synNotch ligands to gelatin polymers to activate synNotch receptors in cells grown on or within a hydrogel
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
SynNotch ligands such as GFP can be conjugated to cell-generated ECM proteins through genetic engineering of fibronectin produced by fibroblasts.
synNotch ligands, such as GFP, can be conjugated to cell-generated ECM proteins via genetic engineering of fibronectin produced by fibroblasts
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
Synthetic Notch receptors are modular synthetic components engineered into mammalian cells to detect neighboring-cell-presented signals and activate prescribed transcriptional programs.
Synthetic Notch (synNotch) receptors are modular synthetic components that are genetically engineered into mammalian cells to detect signals presented by neighboring cells and respond by activating prescribed transcriptional programs.
Synthetic Notch receptors are modular synthetic components engineered into mammalian cells to detect neighboring-cell-presented signals and activate prescribed transcriptional programs.
Synthetic Notch (synNotch) receptors are modular synthetic components that are genetically engineered into mammalian cells to detect signals presented by neighboring cells and respond by activating prescribed transcriptional programs.
Synthetic Notch receptors are modular synthetic components engineered into mammalian cells to detect neighboring-cell-presented signals and activate prescribed transcriptional programs.
Synthetic Notch (synNotch) receptors are modular synthetic components that are genetically engineered into mammalian cells to detect signals presented by neighboring cells and respond by activating prescribed transcriptional programs.
Synthetic Notch receptors are modular synthetic components engineered into mammalian cells to detect neighboring-cell-presented signals and activate prescribed transcriptional programs.
Synthetic Notch (synNotch) receptors are modular synthetic components that are genetically engineered into mammalian cells to detect signals presented by neighboring cells and respond by activating prescribed transcriptional programs.
Synthetic Notch receptors are modular synthetic components engineered into mammalian cells to detect neighboring-cell-presented signals and activate prescribed transcriptional programs.
Synthetic Notch (synNotch) receptors are modular synthetic components that are genetically engineered into mammalian cells to detect signals presented by neighboring cells and respond by activating prescribed transcriptional programs.
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands enabled tissues with up to three distinct phenotypes.
We also patterned tissues comprising cells with up to three distinct phenotypes by engineering cells with two distinct synthetic pathways and culturing them on surfaces microfluidically patterned with two synNotch ligands.
distinct phenotypes 3distinct synthetic pathways 2synNotch ligands 2
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Microcontact printing synNotch ligands onto a surface enables microscale control over synNotch activation in cell monolayers.
To achieve microscale control over synNotch activation in cell monolayers, we microcontact printed synNotch ligands onto a surface.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug material-to-cell-synnotch-ligand-platforms
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
Source:
application demosupports
The technology was used to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns toward engineering muscle tissue with prescribed vascular networks.
We showcase this technology by co-transdifferentiating fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns towards the engineering of muscle tissue with prescribed vascular networks.
Source:
engineering advancesupports
The authors developed a suite of materials that activate synNotch receptors and provide generalizable platforms for user-defined material-to-cell signaling pathways.
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
Source:
Comparisons
Source-backed strengths
The reported system activated synNotch receptors when ligands were covalently linked to gelatin polymers by either enzymatic conjugation or click chemistry, including in cells grown on or within hydrogels. It was further applied to co-transdifferentiate fibroblasts into skeletal muscle or endothelial cell precursors in user-defined spatial patterns, and synNotch ligands such as GFP were also conjugated to cell-generated extracellular matrix through genetic engineering of fibronectin.
Source:
we developed a suite of materials to activate synNotch receptors and serve as generalizable platforms for generating user-defined material-to-cell signaling pathways
Compared with biofunctional nanodot arrays
Material-to-cell synNotch ligand platforms and biofunctional nanodot arrays address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling; same primary input modality: chemical
Compared with LED illumination system
Material-to-cell synNotch ligand platforms and LED illumination system address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling
Strengths here: looks easier to implement in practice.
Compared with Potato virus X nanoparticle shuttle
Material-to-cell synNotch ligand platforms and Potato virus X nanoparticle shuttle address a similar problem space because they share signaling.
Shared frame: same top-level item type; shared target processes: signaling
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.