Source 1review2019Current Pharmaceutical Biotechnology
Toolkit/multi-electrode array recording
multi-electrode array recording
Also known as: MEA, multi-electrode array
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Multi-electrode array recording is an electrophysiological assay method that measures extracellular action potential firing from the retinal ganglion layer. In the cited study, it was used with the array in contact with the retinal ganglion layer to detect light-evoked responses.
Usefulness & Problems
Why this is useful
This method is useful for functionally assessing whether light stimulation elicits neuronal spiking output at the level of retinal ganglion cells. The cited evidence supports its use as a readout of light-induced retinal activity in an ex vivo retinal preparation.
Problem solved
It addresses the need to detect and quantify light-evoked action potential firing from the retinal ganglion layer. In the cited work, it provided a direct electrophysiological readout of whether light-controlled signaling produced spiking responses.
Problem links
Need better screening or enrichment leverage
DerivedMulti-electrode array recording is an electrophysiological assay method that measures extracellular action potential firing from the retinal ganglion layer. In the cited study, it was used with the array in contact with the retinal ganglion layer to detect light-evoked responses.
Need conditional recombination or state switching
DerivedMulti-electrode array recording is an electrophysiological assay method that measures extracellular action potential firing from the retinal ganglion layer. In the cited study, it was used with the array in contact with the retinal ganglion layer to detect light-evoked responses.
Need precise spatiotemporal control with light input
DerivedMulti-electrode array recording is an electrophysiological assay method that measures extracellular action potential firing from the retinal ganglion layer. In the cited study, it was used with the array in contact with the retinal ganglion layer to detect light-evoked responses.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Target processes
recombinationselectionInput: Light
Implementation Constraints
cofactor dependency: requires exogenous cofactorencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The reported configuration placed the multi-electrode array in contact with the retinal ganglion layer. The available evidence does not specify electrode layout, recording conditions, analysis pipeline, or preparation details beyond this placement.
The supplied evidence only establishes that light-evoked firing was recorded from the retinal ganglion layer, without reporting performance metrics such as sensitivity, spatial resolution, signal-to-noise ratio, or throughput. No independent replication or broader benchmarking is provided in the supplied material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2022Journal of Neural Engineering
Ranked Claims
The light-induced response manifests as evoked firing of action potentials recorded from the retinal ganglion layer using a multi-electrode array.
The response manifests as evoked-firing of action potentials and was recorded using a multi-electrode array in contact with the retinal ganglion layer.
The light-induced response manifests as evoked firing of action potentials recorded from the retinal ganglion layer using a multi-electrode array.
The response manifests as evoked-firing of action potentials and was recorded using a multi-electrode array in contact with the retinal ganglion layer.
The light-induced response manifests as evoked firing of action potentials recorded from the retinal ganglion layer using a multi-electrode array.
The response manifests as evoked-firing of action potentials and was recorded using a multi-electrode array in contact with the retinal ganglion layer.
The light-induced response manifests as evoked firing of action potentials recorded from the retinal ganglion layer using a multi-electrode array.
The response manifests as evoked-firing of action potentials and was recorded using a multi-electrode array in contact with the retinal ganglion layer.
The light-induced response manifests as evoked firing of action potentials recorded from the retinal ganglion layer using a multi-electrode array.
The response manifests as evoked-firing of action potentials and was recorded using a multi-electrode array in contact with the retinal ganglion layer.
The light-induced response manifests as evoked firing of action potentials recorded from the retinal ganglion layer using a multi-electrode array.
The response manifests as evoked-firing of action potentials and was recorded using a multi-electrode array in contact with the retinal ganglion layer.
The light-induced response manifests as evoked firing of action potentials recorded from the retinal ganglion layer using a multi-electrode array.
The response manifests as evoked-firing of action potentials and was recorded using a multi-electrode array in contact with the retinal ganglion layer.
For iPS-CM based screening, optogenetic stimulation offers contact independence, avoids electrical stimulation artefacts in multi-electrode array field potential measurements, and allows patterned induction of re-entrant depolarization in 2D cardiomyocyte monolayers.
We show that the advantages of optogenetic stimulation relevant to iPS-CM based screening include independence from contact, elimination of electrical stimulation artefacts in field potential measuring approaches such as the multi-electrode array, and the ability to print re-entrant patterns of depolarization at will on 2D cardiomyocyte monolayers.
Approval Evidence
1 source1 linked approval claimfirst-pass slug multi-electrode-array-recording
recorded using a multi-electrode array in contact with the retinal ganglion layer
Source:
electrophysiology observationsupports
The light-induced response manifests as evoked firing of action potentials recorded from the retinal ganglion layer using a multi-electrode array.
The response manifests as evoked-firing of action potentials and was recorded using a multi-electrode array in contact with the retinal ganglion layer.
Source:
Comparisons
Source-backed strengths
The method directly records evoked action potentials from the retinal ganglion layer using extracellular electrodes. The cited study specifically validated that light-induced responses could be observed as firing recorded by a multi-electrode array.
Compared with droplet microfluidic platform
multi-electrode array recording and droplet microfluidic platform address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with fluorescence recovery after photobleaching
multi-electrode array recording and fluorescence recovery after photobleaching address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with open-source microplate reader
multi-electrode array recording and open-source microplate reader address a similar problem space because they share recombination, selection.
Shared frame: same top-level item type; shared target processes: recombination, selection; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Ranked Citations
- 1.
- 2.