Source 1primary paper2021PLoS ONE
Toolkit/Opto-Kv1(V400D)
Opto-Kv1(V400D)
Also known as: Opto-Kv1 (V400D)
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Opto-Kv1(V400D) is a photoactivatable Kv1 channel construct bearing a dominant-negative pore mutation, V400D. Blue light enables this construct to down-regulate Kv1 currents, providing acute optical control of voltage-gated potassium channel function.
Usefulness & Problems
Why this is useful
This construct is useful for acute, light-dependent perturbation of Kv1 channel activity in neurons and in vivo neural circuits. The cited study indicates that photoactivatable Kv channels can be used to interrogate brain function, behavior, and the roles of normal and disease-causing mutant Kv channels.
Source:
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
Source:
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
Problem solved
Opto-Kv1(V400D) addresses the need for temporally controlled suppression of Kv1 currents without relying solely on constitutive genetic perturbation. It enables blue light-dependent down-regulation of Kv1 channel function for functional studies in neurons and behavior.
Source:
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
Source:
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
Problem links
Need conditional recombination or state switching
DerivedOpto-Kv1(V400D) is a photoactivatable Kv1 channel construct bearing a dominant-negative pore mutation, V400D. Blue light enables this construct to down-regulate Kv1 currents, providing acute optical control of voltage-gated potassium channel function.
Need precise spatiotemporal control with light input
DerivedOpto-Kv1(V400D) is a photoactivatable Kv1 channel construct bearing a dominant-negative pore mutation, V400D. Blue light enables this construct to down-regulate Kv1 currents, providing acute optical control of voltage-gated potassium channel function.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
The construct contains a dominant-negative pore mutation, V400D, in an Opto-Kv1 background. The provided evidence supports use with blue light and reports in vivo targeting to Kv1.3-expressing mitral cells in the mouse olfactory bulb, but it does not specify construct architecture, delivery method, promoter, or cofactor requirements.
The supplied evidence is limited to one primary study and does not provide quantitative electrophysiological performance metrics, kinetics, reversibility, or spectral details beyond blue light dependence. Practical performance across cell types, expression contexts, and non-olfactory circuits is not described in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The developed photoactivatable Kv channels provide ways to interrogate neural circuits in vivo and examine roles of normal and disease-causing mutant Kv channels in brain function and behavior.
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
The developed photoactivatable Kv channels provide ways to interrogate neural circuits in vivo and examine roles of normal and disease-causing mutant Kv channels in brain function and behavior.
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
The developed photoactivatable Kv channels provide ways to interrogate neural circuits in vivo and examine roles of normal and disease-causing mutant Kv channels in brain function and behavior.
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
The developed photoactivatable Kv channels provide ways to interrogate neural circuits in vivo and examine roles of normal and disease-causing mutant Kv channels in brain function and behavior.
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
The developed photoactivatable Kv channels provide ways to interrogate neural circuits in vivo and examine roles of normal and disease-causing mutant Kv channels in brain function and behavior.
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
The developed photoactivatable Kv channels provide ways to interrogate neural circuits in vivo and examine roles of normal and disease-causing mutant Kv channels in brain function and behavior.
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
The developed photoactivatable Kv channels provide ways to interrogate neural circuits in vivo and examine roles of normal and disease-causing mutant Kv channels in brain function and behavior.
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
In mice with Opto-Kv1(V400D) targeted to Kv1.3-expressing mitral cells of the olfactory bulb, blue light exposure for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
we targeted Opto-Kv1 (V400D) expression to Kv1.3-expressing mitral cells of the olfactory bulb in mice. Exposure of the bulb to blue light for 2-3 hours produced a significant increase in sensitivity to novel odors after initial habituation to a similar odor
blue light exposure duration 2-3 hours
In mice with Opto-Kv1(V400D) targeted to Kv1.3-expressing mitral cells of the olfactory bulb, blue light exposure for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
we targeted Opto-Kv1 (V400D) expression to Kv1.3-expressing mitral cells of the olfactory bulb in mice. Exposure of the bulb to blue light for 2-3 hours produced a significant increase in sensitivity to novel odors after initial habituation to a similar odor
blue light exposure duration 2-3 hours
In mice with Opto-Kv1(V400D) targeted to Kv1.3-expressing mitral cells of the olfactory bulb, blue light exposure for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
we targeted Opto-Kv1 (V400D) expression to Kv1.3-expressing mitral cells of the olfactory bulb in mice. Exposure of the bulb to blue light for 2-3 hours produced a significant increase in sensitivity to novel odors after initial habituation to a similar odor
blue light exposure duration 2-3 hours
In mice with Opto-Kv1(V400D) targeted to Kv1.3-expressing mitral cells of the olfactory bulb, blue light exposure for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
we targeted Opto-Kv1 (V400D) expression to Kv1.3-expressing mitral cells of the olfactory bulb in mice. Exposure of the bulb to blue light for 2-3 hours produced a significant increase in sensitivity to novel odors after initial habituation to a similar odor
blue light exposure duration 2-3 hours
In mice with Opto-Kv1(V400D) targeted to Kv1.3-expressing mitral cells of the olfactory bulb, blue light exposure for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
we targeted Opto-Kv1 (V400D) expression to Kv1.3-expressing mitral cells of the olfactory bulb in mice. Exposure of the bulb to blue light for 2-3 hours produced a significant increase in sensitivity to novel odors after initial habituation to a similar odor
blue light exposure duration 2-3 hours
In mice with Opto-Kv1(V400D) targeted to Kv1.3-expressing mitral cells of the olfactory bulb, blue light exposure for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
we targeted Opto-Kv1 (V400D) expression to Kv1.3-expressing mitral cells of the olfactory bulb in mice. Exposure of the bulb to blue light for 2-3 hours produced a significant increase in sensitivity to novel odors after initial habituation to a similar odor
blue light exposure duration 2-3 hours
In mice with Opto-Kv1(V400D) targeted to Kv1.3-expressing mitral cells of the olfactory bulb, blue light exposure for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
we targeted Opto-Kv1 (V400D) expression to Kv1.3-expressing mitral cells of the olfactory bulb in mice. Exposure of the bulb to blue light for 2-3 hours produced a significant increase in sensitivity to novel odors after initial habituation to a similar odor
blue light exposure duration 2-3 hours
Opto-Kv1(V400D) can down-regulate Kv1 currents in a blue light-dependent manner.
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Opto-Kv1(V400D) can down-regulate Kv1 currents in a blue light-dependent manner.
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Opto-Kv1(V400D) can down-regulate Kv1 currents in a blue light-dependent manner.
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Opto-Kv1(V400D) can down-regulate Kv1 currents in a blue light-dependent manner.
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Opto-Kv1(V400D) can down-regulate Kv1 currents in a blue light-dependent manner.
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Opto-Kv1(V400D) can down-regulate Kv1 currents in a blue light-dependent manner.
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Opto-Kv1(V400D) can down-regulate Kv1 currents in a blue light-dependent manner.
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Blue light strongly induces current expression of Opto-Kv1 channels in Xenopus oocytes, mammalian cells, and neurons.
Recording of Opto-Kv1 channels expressed in Xenopus oocytes, mammalian cells, and neurons show that blue light strongly induces the current expression of Opto-Kv1 channels in all systems tested.
Blue light strongly induces current expression of Opto-Kv1 channels in Xenopus oocytes, mammalian cells, and neurons.
Recording of Opto-Kv1 channels expressed in Xenopus oocytes, mammalian cells, and neurons show that blue light strongly induces the current expression of Opto-Kv1 channels in all systems tested.
Blue light strongly induces current expression of Opto-Kv1 channels in Xenopus oocytes, mammalian cells, and neurons.
Recording of Opto-Kv1 channels expressed in Xenopus oocytes, mammalian cells, and neurons show that blue light strongly induces the current expression of Opto-Kv1 channels in all systems tested.
Blue light strongly induces current expression of Opto-Kv1 channels in Xenopus oocytes, mammalian cells, and neurons.
Recording of Opto-Kv1 channels expressed in Xenopus oocytes, mammalian cells, and neurons show that blue light strongly induces the current expression of Opto-Kv1 channels in all systems tested.
Blue light strongly induces current expression of Opto-Kv1 channels in Xenopus oocytes, mammalian cells, and neurons.
Recording of Opto-Kv1 channels expressed in Xenopus oocytes, mammalian cells, and neurons show that blue light strongly induces the current expression of Opto-Kv1 channels in all systems tested.
Blue light strongly induces current expression of Opto-Kv1 channels in Xenopus oocytes, mammalian cells, and neurons.
Recording of Opto-Kv1 channels expressed in Xenopus oocytes, mammalian cells, and neurons show that blue light strongly induces the current expression of Opto-Kv1 channels in all systems tested.
Blue light strongly induces current expression of Opto-Kv1 channels in Xenopus oocytes, mammalian cells, and neurons.
Recording of Opto-Kv1 channels expressed in Xenopus oocytes, mammalian cells, and neurons show that blue light strongly induces the current expression of Opto-Kv1 channels in all systems tested.
The authors developed Opto-Kv1, a light-regulated Kv1 channel created by fusing the LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of a Kv1 subunit.
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
The authors developed Opto-Kv1, a light-regulated Kv1 channel created by fusing the LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of a Kv1 subunit.
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
The authors developed Opto-Kv1, a light-regulated Kv1 channel created by fusing the LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of a Kv1 subunit.
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
The authors developed Opto-Kv1, a light-regulated Kv1 channel created by fusing the LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of a Kv1 subunit.
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
The authors developed Opto-Kv1, a light-regulated Kv1 channel created by fusing the LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of a Kv1 subunit.
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
The authors developed Opto-Kv1, a light-regulated Kv1 channel created by fusing the LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of a Kv1 subunit.
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
The authors developed Opto-Kv1, a light-regulated Kv1 channel created by fusing the LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of a Kv1 subunit.
we have developed a novel optogenetic technology to acutely regulate Kv channel expression with light by fusing the light-sensitive LOV domain of Vaucheria frigida Aureochrome 1 to the N-terminus of the Kv1 subunit protein to make an Opto-Kv1 channel
Approval Evidence
1 source3 linked approval claimsfirst-pass slug opto-kv1-v400d
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Source:
application scopesupports
The developed photoactivatable Kv channels provide ways to interrogate neural circuits in vivo and examine roles of normal and disease-causing mutant Kv channels in brain function and behavior.
we have developed novel photoactivatable Kv channels that provide new ways to interrogate neural circuits in vivo and to examine the roles of normal and disease-causing mutant Kv channels in brain function and behavior
Source:
behavioral effectsupports
In mice with Opto-Kv1(V400D) targeted to Kv1.3-expressing mitral cells of the olfactory bulb, blue light exposure for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
we targeted Opto-Kv1 (V400D) expression to Kv1.3-expressing mitral cells of the olfactory bulb in mice. Exposure of the bulb to blue light for 2-3 hours produced a significant increase in sensitivity to novel odors after initial habituation to a similar odor
Source:
dominant negative regulationsupports
Opto-Kv1(V400D) can down-regulate Kv1 currents in a blue light-dependent manner.
an Opto-Kv1 construct containing a dominant-negative pore mutation (Opto-Kv1(V400D)) can be used to down-regulate Kv1 currents in a blue light-dependent manner
Source:
Comparisons
Source-backed strengths
The key demonstrated strength is blue light-dependent down-regulation of Kv1 currents by a dominant-negative Kv1 construct. In vivo relevance is supported by a behavioral result in mice, where targeting Opto-Kv1(V400D) to Kv1.3-expressing olfactory bulb mitral cells and applying blue light for 2-3 hours increased sensitivity to novel odors after habituation to a similar odor.
Opto-Kv1(V400D) and modular light-controlled skeletal muscle-powered bioactuator address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Compared with Opto-Casp8-V2
Opto-Kv1(V400D) and Opto-Casp8-V2 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Compared with pcVP16
Opto-Kv1(V400D) and pcVP16 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Ranked Citations
- 1.