Source 1primary paper1992The Plant Journal
Toolkit/parsley protoplast transient expression system
parsley protoplast transient expression system
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The parsley protoplast transient expression system is a plant cell-based transient reporter assay used to test light-regulated transcription from introduced promoter constructs. In the cited study, chalcone synthase (CHS) promoter-GUS constructs in parsley protoplasts reproduced the same type of photoregulation observed for the endogenous CHS gene.
Usefulness & Problems
Why this is useful
This system is useful for assessing whether introduced plant promoter-reporter constructs recapitulate endogenous light-responsive gene regulation in a transient cellular context. The available evidence specifically supports its use for testing photoregulation of CHS promoter-GUS constructs in parsley protoplasts.
Problem solved
It addresses the problem of functionally testing light-regulated promoter activity without requiring stable transformation. The cited evidence indicates that it can determine whether CHS promoter constructs preserve endogenous-like photoregulatory behavior in protoplasts.
Problem links
Need precise spatiotemporal control with light input
DerivedThe parsley protoplast transient expression system is a plant cell-based transient reporter assay used to test light-regulated gene expression. In the cited study, CHS promoter-GUS constructs in parsley protoplasts reproduced the same type of photoregulation observed for the endogenous chalcone synthase (CHS) gene.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
light-dependent transcriptional regulationlight-dependent transcriptional regulationpromoter-reporter assay readoutpromoter-reporter assay readouttransient transgene expressiontransient transgene expressionTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The system uses parsley protoplasts and transiently introduced CHS promoter-GUS reporter constructs. Beyond this general construct and host context, the supplied evidence does not specify protoplast preparation, transfection method, light regime, or reporter assay conditions.
The evidence is limited to a single reported application involving CHS promoter-GUS constructs and endogenous CHS photoregulation in parsley protoplasts. No quantitative performance metrics, dynamic range, throughput, or validation across other promoters, genes, or plant species are provided in the supplied evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
Approval Evidence
1 source1 linked approval claimfirst-pass slug parsley-protoplast-transient-expression-system
When tested in the parsley protoplast transient expression system
Source:
assay recapitulationsupports
In the parsley protoplast transient expression system, both CHS promoter-GUS constructs yielded the same type of photoregulation as the endogenous CHS gene.
When tested in the parsley protoplast transient expression system, both constructs yielded the same type of photoregulation as observed for the endogenous CHS gene.
Source:
Comparisons
Source-backed strengths
The reported strength is biological recapitulation: both CHS promoter-GUS constructs showed the same type of photoregulation as the endogenous CHS gene in the parsley protoplast transient expression system. This supports the assay's relevance for transient analysis of light-responsive transcriptional control.
Compared with native green gel system
parsley protoplast transient expression system and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with plant transcriptome profiling
parsley protoplast transient expression system and plant transcriptome profiling address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with rapid transient expression assay system
parsley protoplast transient expression system and rapid transient expression assay system address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: transient transgene expression; same primary input modality: light
Ranked Citations
- 1.