Source 1primary paper1989Proceedings of the National Academy of Sciences
Toolkit/rapid transient expression assay system
rapid transient expression assay system
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The rapid transient expression assay system is a microprojectile-mediated transient gene transfer method developed to study DNA sequences involved in phytochrome-regulated phy gene expression. It enables promoter construct readout in less than 24 hours after particle bombardment and is used to assess light-regulated transcriptional responses.
Usefulness & Problems
Why this is useful
This assay is useful for rapid functional testing of promoter and other cis-regulatory DNA sequences linked to phytochrome-responsive gene expression. Its main value is shortening the time required to evaluate light-dependent transcriptional regulation after DNA delivery.
Problem solved
It addresses the need for a fast assay to analyze DNA sequences controlling phytochrome-regulated phy gene expression without waiting for stable transformation. The cited work specifically positions it for studying phytochrome-dependent transcriptional regulation and negative feedback on phy genes.
Problem links
Need precise spatiotemporal control with light input
DerivedThe rapid transient expression assay system is a microprojectile-mediated gene transfer assay used to study DNA sequences that control phytochrome-regulated phy gene expression. It enables analysis of introduced promoter-reporter constructs within less than 24 hours after particle bombardment and reports light-dependent down-regulation in responsive monocot species.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
light-regulated transcriptional controllight-regulated transcriptional controlphytochrome-mediated negative feedback regulationphytochrome-mediated negative feedback regulationtransient transgene expressiontransient transgene expressionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
Implementation is based on microprojectile-mediated gene transfer by particle bombardment of introduced constructs. The evidence supports use for promoter-reporter style analysis of phy gene regulatory sequences, with assayable expression detected within 24 hours; no additional details on reporters, vectors, or growth conditions are provided in the supplied material.
The supplied evidence is limited to a single 1989 source and does not provide quantitative performance metrics, sensitivity, dynamic range, or comparison to alternative assays. Evidence for species scope, construct types, and reproducibility beyond the reported particle bombardment context is not provided here.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
time to assayable expression 24 hr
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
Phytochrome controls transcription of its own phy genes in a negative feedback fashion.
The regulatory photoreceptor phytochrome controls the transcription of its own phy genes in a negative feedback fashion.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, but no expression is detected in tobacco, cucumber, and Arabidopsis thaliana.
The introduced oat phy-CAT fusion gene is expressed and down-regulated by white light in barley, rice, and oat, whereas no expression is detected in three dicots tested, tobacco, cucumber, and Arabidopsis thaliana.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
The transduction pathway components and promoter sequences involved in autoregulation of phy expression are evolutionarily conserved between oat and rice.
These data indicate that the transduction pathway components and promoter sequences involved in autoregulation of phy expression have been evolutionarily conserved between oat and rice.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
In bombarded rice shoots, repression of the heterologous oat phy-CAT gene is red/far-red light reversible, indicating regulation by phytochrome in parallel with endogenous rice phy genes.
In bombarded rice shoots, red/far-red light-reversible repression of expression of the heterologous oat phy-CAT gene shows that it is regulated by phytochrome in a manner parallel to that of the endogenous rice phy genes.
Approval Evidence
1 source3 linked approval claimsfirst-pass slug rapid-transient-expression-assay-system
develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes
Source:
assay capabilitysupports
Microprojectile-mediated gene transfer was used to develop a rapid transient expression assay system for studying DNA sequences involved in phytochrome-regulated phy gene expression.
We have exploited microprojectile-mediated gene transfer to develop a rapid transient expression assay system for the study of DNA sequences involved in the phytochrome-regulated expression of these genes.
Source:
assay timingsupports
Expression from the introduced construct is assayable in less than 24 hours after bombardment.
Expression is assayable in less than 24 hr from bombardment.
Source:
method feasibilitysupports
High-velocity microprojectile-mediated gene transfer is feasible for rapid analysis of light-controlled monocot gene promoters in monocot tissues previously recalcitrant to such studies.
The experiments show the feasibility of using high-velocity microprojectile-mediated gene transfer for the rapid analysis of light-controlled monocot gene promoters in monocot tissues that until now have been recalcitrant to such studies.
Source:
Comparisons
Source-backed strengths
The principal demonstrated strength is speed, because expression from introduced constructs is assayable in less than 24 hours after bombardment. It was specifically developed for functional analysis of DNA sequences involved in phytochrome-regulated expression, providing a direct transient readout of light-responsive transcription.
Compared with native green gel system
rapid transient expression assay system and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with parsley protoplast transient expression system
rapid transient expression assay system and parsley protoplast transient expression system address a similar problem space.
Shared frame: same top-level item type; shared mechanisms: transient transgene expression; same primary input modality: light
Compared with plant transcriptome profiling
rapid transient expression assay system and plant transcriptome profiling address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.