Source 1review2015Quarterly Reviews of Biophysics
Toolkit/photoswitchable fluorophores
photoswitchable fluorophores
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Some require specific transitions and states that can be found only in certain fluorophore subfamilies, such as photoswitchable fluorophores, while other variants can be realized with standard fluorescent labels.
Usefulness & Problems
Why this is useful
Photoswitchable fluorophores provide distinct emission states, such as on and off states, that some nanoscopy methods exploit to separate nearby features in time.; enabling nanoscopy variants that require specific fluorophore state transitions
Source:
Photoswitchable fluorophores provide distinct emission states, such as on and off states, that some nanoscopy methods exploit to separate nearby features in time.
Source:
enabling nanoscopy variants that require specific fluorophore state transitions
Problem solved
They enable the transient state control needed for certain super-resolution modalities to make adjacent molecules discernible.; providing switchable emission states needed to distinguish adjacent fluorescent features sequentially in time
Source:
They enable the transient state control needed for certain super-resolution modalities to make adjacent molecules discernible.
Source:
providing switchable emission states needed to distinguish adjacent fluorescent features sequentially in time
Problem links
providing switchable emission states needed to distinguish adjacent fluorescent features sequentially in time
LiteratureThey enable the transient state control needed for certain super-resolution modalities to make adjacent molecules discernible.
Source:
They enable the transient state control needed for certain super-resolution modalities to make adjacent molecules discernible.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
light-dependent state transitionsphotoswitchingreversible or transient emission-state switchingTechniques
No technique tags yet.
Target processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: actuator
They must be used in imaging setups and method variants that depend on specific fluorophore state transitions.; must provide the specific transitions and states required by the imaging modality
They are not a universal requirement across all nanoscopy variants, because the abstract states that some other variants can use standard fluorescent labels.; restricted to certain fluorophore subfamilies; not required by all nanoscopy variants
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Some nanoscopy variants require specific fluorophore transitions and states found only in certain fluorophore subfamilies such as photoswitchable fluorophores, whereas other variants can be realized with standard fluorescent labels.
Some require specific transitions and states that can be found only in certain fluorophore subfamilies, such as photoswitchable fluorophores, while other variants can be realized with standard fluorescent labels.
Approval Evidence
1 source1 linked approval claimfirst-pass slug photoswitchable-fluorophores
Some require specific transitions and states that can be found only in certain fluorophore subfamilies, such as photoswitchable fluorophores, while other variants can be realized with standard fluorescent labels.
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requirementsupports
Some nanoscopy variants require specific fluorophore transitions and states found only in certain fluorophore subfamilies such as photoswitchable fluorophores, whereas other variants can be realized with standard fluorescent labels.
Some require specific transitions and states that can be found only in certain fluorophore subfamilies, such as photoswitchable fluorophores, while other variants can be realized with standard fluorescent labels.
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Comparisons
Source-stated alternatives
The abstract contrasts photoswitchable fluorophores with standard fluorescent labels for variants that do not require specialized switching behavior.
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The abstract contrasts photoswitchable fluorophores with standard fluorescent labels for variants that do not require specialized switching behavior.
Source-backed strengths
support specific transitions and states required by some nanoscopy variants
Source:
support specific transitions and states required by some nanoscopy variants
Compared with phosphorothioate-caged antisense oligonucleotides
photoswitchable fluorophores and phosphorothioate-caged antisense oligonucleotides address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with photo-sensitive circular gRNAs
photoswitchable fluorophores and photo-sensitive circular gRNAs address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with RNA aptamer
photoswitchable fluorophores and RNA aptamer address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.