Source 1primary paper2020Angewandte Chemie International Edition
Toolkit/RNA aptamer
RNA aptamer
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This tool is an RNA aptamer-based component for light-controlled, reversible gene transactivation in a CRISPR/dCas9-based system. It is built on the interaction between the photoreceptor PAL and an RNA aptamer to regulate gene expression with light.
Usefulness & Problems
Why this is useful
The platform provides a modular building block for synthetic biological circuit design and is described as broadly applicable. It is also reported to reduce the coding space required for genetic manipulation relative to more protein-heavy implementations.
Source:
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
Problem solved
This tool addresses the problem of achieving reversible, light-dependent control of CRISPR/dCas9-mediated gene activation using a compact genetic component. The cited work specifically positions it as a way to implement transactivation with strong light responsiveness and minimal dark-state activity.
Problem links
Need controllable genome or transcript editing
DerivedThis tool is an RNA aptamer-based component for light-controlled, reversible gene transactivation in a CRISPR/dCas9-based system. It is built on the interaction between the photoreceptor PAL and an RNA aptamer to regulate gene expression with light.
Need precise spatiotemporal control with light input
DerivedThis tool is an RNA aptamer-based component for light-controlled, reversible gene transactivation in a CRISPR/dCas9-based system. It is built on the interaction between the photoreceptor PAL and an RNA aptamer to regulate gene expression with light.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
editingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
The construct is based on coupling the photoreceptor PAL to an RNA aptamer within a CRISPR/dCas9 gene activation framework. The available evidence supports light-controlled operation and modular design, but does not specify wavelengths, expression systems, delivery methods, or exact construct architecture.
The supplied evidence does not provide quantitative performance metrics, illumination parameters, target genes, or organism-specific validation details. Independent replication beyond the cited study is not provided in the evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform is broadly applicable and adds to modular building blocks for synthetic biological circuit design.
It adds to the current set of modular building blocks for synthetic biological circuit design and is broadly applicable.
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform significantly reduces the coding space required for genetic manipulation.
This platform significantly reduces the coding space required for genetic manipulation
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The platform provides a strong on-switch with almost no residual activity in the dark.
provides a strong on-switch with almost no residual activity in the dark
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
Approval Evidence
1 source1 linked approval claimfirst-pass slug rna-aptamer
Building on the interaction of the photoreceptor PAL with an RNA aptamer
Source:
tool descriptionsupports
The paper describes a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression built on the interaction of PAL with an RNA aptamer.
Building on the interaction of the photoreceptor PAL with an RNA aptamer, we describe a variation of the CRISPR/dCAS9 system for light-controlled activation of gene expression.
Source:
Comparisons
Source-backed strengths
The source reports a strong on-switch with almost no residual activity in the dark, indicating high dynamic separation between illuminated and dark conditions. The design is also presented as modular and broadly applicable, with the added advantage of reduced coding space.
Source:
provides a strong on-switch with almost no residual activity in the dark
Compared with antisense oligonucleotides
RNA aptamer and antisense oligonucleotides address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
Compared with photo-sensitive circular gRNAs
RNA aptamer and photo-sensitive circular gRNAs address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
Compared with small regulatory RNAs
RNA aptamer and small regulatory RNAs address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
Ranked Citations
- 1.