Source 1review2002Journal of the American Society of Nephrology
Toolkit/protein truncation test
protein truncation test
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
More recently, with the use of long-range PCR, denaturing HPLC (DHPLC), and the protein truncation test, mutations in the duplicated region of the PKD1 gene have been identified
Usefulness & Problems
Why this is useful
The protein truncation test is cited as part of the approach used to identify PKD1 mutations in duplicated sequence regions.; detecting truncating or disruptive mutations during PKD1 analysis
Source:
The protein truncation test is cited as part of the approach used to identify PKD1 mutations in duplicated sequence regions.
Source:
detecting truncating or disruptive mutations during PKD1 analysis
Problem solved
It helps analyze PKD1 in a setting where mutational analysis is hindered by duplicated genomic sequence.; supports mutation identification in a gene with duplicated regions and many inactivating variants
Source:
It helps analyze PKD1 in a setting where mutational analysis is hindered by duplicated genomic sequence.
Source:
supports mutation identification in a gene with duplicated regions and many inactivating variants
Problem links
supports mutation identification in a gene with duplicated regions and many inactivating variants
LiteratureIt helps analyze PKD1 in a setting where mutational analysis is hindered by duplicated genomic sequence.
Source:
It helps analyze PKD1 in a setting where mutational analysis is hindered by duplicated genomic sequence.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
No mechanism tags yet.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The review places it alongside long-range PCR and DHPLC rather than describing it as a standalone assay.; used as part of a multi-method mutation-analysis workflow
the abstract does not define its detection scope or sensitivity
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Long-range PCR, DHPLC, and the protein truncation test enabled identification of mutations in the duplicated region of PKD1.
with the use of long-range PCR, denaturing HPLC (DHPLC), and the protein truncation test, mutations in the duplicated region of the PKD1 gene have been identified
Positional cloning was used to clone PKD2.
The second ADPKD gene, PKD2, was cloned in 1996 by positional cloning
Approval Evidence
1 source1 linked approval claimfirst-pass slug protein-truncation-test
More recently, with the use of long-range PCR, denaturing HPLC (DHPLC), and the protein truncation test, mutations in the duplicated region of the PKD1 gene have been identified
Source:
method use summarysupports
Long-range PCR, DHPLC, and the protein truncation test enabled identification of mutations in the duplicated region of PKD1.
with the use of long-range PCR, denaturing HPLC (DHPLC), and the protein truncation test, mutations in the duplicated region of the PKD1 gene have been identified
Source:
Comparisons
Source-stated alternatives
The review mentions long-range PCR and DHPLC as related methods used in the same context.
Source:
The review mentions long-range PCR and DHPLC as related methods used in the same context.
Source-backed strengths
explicitly included in the method set that enabled PKD1 mutation identification
Source:
explicitly included in the method set that enabled PKD1 mutation identification
Compared with Langendorff perfused heart electrical recordings
protein truncation test and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with native green gel system
protein truncation test and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
protein truncation test and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.