Source 1primary paper1996Genome Research
Toolkit/real-time quantitative PCR
real-time quantitative PCR
Also known as: real-time PCR, real time quantitative PCR
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
We have developed a novel "real time" quantitative PCR method.
Usefulness & Problems
Why this is useful
This method measures PCR product accumulation in real time to quantify starting gene copy number. The abstract presents it as a quantitative PCR format with accurate and reproducible readout.; quantitation of gene copies; higher-throughput quantitative PCR assays; closed-tube PCR quantification without post-PCR handling
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This method measures PCR product accumulation in real time to quantify starting gene copy number. The abstract presents it as a quantitative PCR format with accurate and reproducible readout.
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quantitation of gene copies
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higher-throughput quantitative PCR assays
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closed-tube PCR quantification without post-PCR handling
Problem solved
It avoids post-PCR sample handling, which the abstract says helps prevent carry-over contamination and makes assays faster and higher throughput. It also supports quantitation across at least five orders of magnitude.; reduces need for post-PCR sample handling; reduces potential PCR product carry-over contamination; provides accurate and reproducible quantitation over a large dynamic range
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It avoids post-PCR sample handling, which the abstract says helps prevent carry-over contamination and makes assays faster and higher throughput. It also supports quantitation across at least five orders of magnitude.
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reduces need for post-PCR sample handling
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reduces potential PCR product carry-over contamination
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provides accurate and reproducible quantitation over a large dynamic range
Problem links
provides accurate and reproducible quantitation over a large dynamic range
LiteratureIt avoids post-PCR sample handling, which the abstract says helps prevent carry-over contamination and makes assays faster and higher throughput. It also supports quantitation across at least five orders of magnitude.
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It avoids post-PCR sample handling, which the abstract says helps prevent carry-over contamination and makes assays faster and higher throughput. It also supports quantitation across at least five orders of magnitude.
reduces need for post-PCR sample handling
LiteratureIt avoids post-PCR sample handling, which the abstract says helps prevent carry-over contamination and makes assays faster and higher throughput. It also supports quantitation across at least five orders of magnitude.
Source:
It avoids post-PCR sample handling, which the abstract says helps prevent carry-over contamination and makes assays faster and higher throughput. It also supports quantitation across at least five orders of magnitude.
reduces potential PCR product carry-over contamination
LiteratureIt avoids post-PCR sample handling, which the abstract says helps prevent carry-over contamination and makes assays faster and higher throughput. It also supports quantitation across at least five orders of magnitude.
Source:
It avoids post-PCR sample handling, which the abstract says helps prevent carry-over contamination and makes assays faster and higher throughput. It also supports quantitation across at least five orders of magnitude.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The assay requires PCR and a dual-labeled fluorogenic probe identified in the abstract as a TaqMan probe. The abstract does not specify instrument details.; uses a dual-labeled fluorogenic probe
Validation breadth across biological contexts is still narrow. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Compared with other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, which helps prevent PCR product carry-over contamination and makes assays faster and higher throughput.
Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays.
Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
Real-time quantitative PCR has a dynamic range for starting target molecule determination of at least five orders of magnitude.
The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude).
dynamic range 5 orders_of_magnitude
Real-time quantitative PCR measures PCR product accumulation through a dual-labeled fluorogenic probe identified as the TaqMan probe.
The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe).
The paper introduces a novel real-time quantitative PCR method.
We have developed a novel "real time" quantitative PCR method.
The real-time quantitative PCR method provides very accurate and reproducible quantitation of gene copies.
This method provides very accurate and reproducible quantitation of gene copies.
accuracy very accuratereproducibility reproducible
Approval Evidence
3 sources6 linked approval claimsfirst-pass slugs real-time-pcr, real-time-polymerase-chain-reaction, real-time-quantitative-pcr
in a cohort of 64 patients by real time PCR
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Using real time polymerase chain reaction and immunohistochemistry techniques
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We have developed a novel "real time" quantitative PCR method.
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comparative advantagesupports
Compared with other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, which helps prevent PCR product carry-over contamination and makes assays faster and higher throughput.
Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays.
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comparative advantagesupports
Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
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dynamic rangesupports
Real-time quantitative PCR has a dynamic range for starting target molecule determination of at least five orders of magnitude.
The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude).
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mechanismsupports
Real-time quantitative PCR measures PCR product accumulation through a dual-labeled fluorogenic probe identified as the TaqMan probe.
The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe).
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method introductionsupports
The paper introduces a novel real-time quantitative PCR method.
We have developed a novel "real time" quantitative PCR method.
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performancesupports
The real-time quantitative PCR method provides very accurate and reproducible quantitation of gene copies.
This method provides very accurate and reproducible quantitation of gene copies.
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Comparisons
Source-stated alternatives
The abstract contrasts the method with other current quantitative PCR methods that require post-PCR handling and are more labor-intensive.
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The abstract contrasts the method with other current quantitative PCR methods that require post-PCR handling and are more labor-intensive.
Source-backed strengths
very accurate and reproducible quantitation; at least five orders of magnitude dynamic range; faster and higher throughput than other quantitative PCR methods; less labor-intensive than current quantitative PCR methods
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very accurate and reproducible quantitation
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at least five orders of magnitude dynamic range
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faster and higher throughput than other quantitative PCR methods
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less labor-intensive than current quantitative PCR methods
Compared with Langendorff perfused heart electrical recordings
real-time quantitative PCR and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: appears more independently replicated; looks easier to implement in practice.
Compared with native green gel system
real-time quantitative PCR and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: appears more independently replicated; looks easier to implement in practice.
real-time quantitative PCR and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: appears more independently replicated; looks easier to implement in practice.
Ranked Citations
- 1.