Source 1primary paper2025PPR
Toolkit/red light-inducible recombinase library
red light-inducible recombinase library
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The red light-inducible recombinase library is a set of light-responsive recombination constructs reported in mammalian cells. It was applied to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
Usefulness & Problems
Why this is useful
This library is useful for controlling recombination with light in mammalian cells and for linking optical inputs to spatially organized cell-fate outputs. The reported application to patterned myogenesis indicates utility for cell patterning experiments.
Source:
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
Source:
We developed a library of red light-inducible recombinases
Problem solved
It addresses the problem of inducing recombination in a light-dependent manner in mammalian cells. The available evidence specifically supports its use for directing patterned myogenesis in a mesenchymal fibroblast-like cell line.
Source:
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
Source:
We developed a library of red light-inducible recombinases
Problem links
expands the limited set of orthogonally addressable light-inducible effectors for mammalian multichromatic circuits
LiteratureIt addresses the limited number of orthogonally addressable optogenetic switches and effectors available for multichromatic mammalian gene regulation.
Source:
It addresses the limited number of orthogonally addressable optogenetic switches and effectors available for multichromatic mammalian gene regulation.
Published Workflows
Objective: Develop and multiplex red light-inducible recombinases for multichromatic gene regulation, Boolean logic, and spatial control of cell fate in mammalian cells.
Why it works: The abstract states that photosensory domains fused to split proteins can tightly modulate protein activity and gene expression, and that multiplexing these recombinases with BLADE addresses the limited number of uniquely excitable light-inducible domains.
photosensory-domain control of split protein activityDNA excision by recombinaseslibrary developmentmultiplexing
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Mechanisms
light-inducible recombinationTechniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensorswitch architecture: split
The constructs were reported in mammalian cells and used in a mesenchymal fibroblast-like cell line. The available evidence does not provide construct architecture, cofactor requirements, delivery method, or expression-system details.
The supplied evidence does not specify the recombinase identities, red-light sensing components, illumination wavelengths, dynamic range, background activity, or kinetics. Validation is currently limited here to a reported mammalian-cell application, with no independent replication provided.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
Approval Evidence
1 source3 linked approval claimsfirst-pass slug red-light-inducible-recombinase-library
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
Source:
applicationsupports
The red light-inducible recombinase library was used to direct patterned myogenesis in a mesenchymal fibroblast-like cell line.
We developed a library of red light-inducible recombinases and directed patterned myogenesis in a mesenchymal fibroblast-like cell line.
Source:
platform integrationsupports
The authors multiplexed light-inducible recombinases with the BLADE platform to address the limited number of light-inducible domains responding to unique excitation spectra.
To address the limited number of light-inducible domains (LIDs) responding to unique excitation spectra, we multiplexed light-inducible recombinases with our ‘Boolean Logic and Arithmetic through DNA Excision’ (BLADE) platform.
Source:
tool developmentsupports
The authors developed a library of red light-inducible recombinases.
We developed a library of red light-inducible recombinases
Source:
Comparisons
Source-stated alternatives
The source contrasts this library with existing light-inducible split protein systems that perform well individually but are scarce as multichromatic, orthogonal systems in mammalian cells.
Source:
The source contrasts this library with existing light-inducible split protein systems that perform well individually but are scarce as multichromatic, orthogonal systems in mammalian cells.
Source-backed strengths
The main demonstrated strength is that the library enabled patterned myogenesis in mammalian cells. The source title also indicates use in controlling cell fate, Boolean logic, and cell patterning, but the provided evidence does not supply further performance details.
red light-inducible recombinase library and modular light-controlled skeletal muscle-powered bioactuator address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Compared with Opto-Casp8-V2
red light-inducible recombinase library and Opto-Casp8-V2 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Compared with pcVP16
red light-inducible recombinase library and pcVP16 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Ranked Citations
- 1.