Source 1primary paper2014Plant and Cell Physiology
Toolkit/RhVI1 promoter
RhVI1 promoter
Also known as: pRhVI1, promoter of RhVI1
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The RhVI1 promoter is an 895 bp promoter fragment from Rosa hybrida Vacuolar Invertase 1 that mediates transcriptional responses to light, sugars, and gibberellins. A 468 bp subfragment is sufficient for reporter activation by these inputs, and a 127 bp region from -595 to -468 bp is critical for combinatorial regulation by light with sugar or gibberellin signals.
Usefulness & Problems
Why this is useful
This promoter is useful as a plant cis-regulatory element for coupling transgene expression to environmental and metabolic inputs, specifically light, sugar, and gibberellin cues. The mapped responsive subregions provide a basis for constructing compact promoter modules and for studying signal integration in transcriptional control.
Source:
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
Problem solved
The RhVI1 promoter helps address the problem of how to obtain transcriptional regulation that integrates multiple plant signals rather than responding to a single input. It also provides a defined promoter system for dissecting the cis-regulatory basis of synergistic light-sugar and light-gibberellin responses.
Problem links
Need precise spatiotemporal control with light input
DerivedThe RhVI1 promoter is an 895 bp promoter fragment from Rosa hybrida Vacuolar Invertase 1 that mediates transcriptional responses to light, sugars, and gibberellins. A 468 bp subfragment is sufficient for reporter activation by these inputs, and a 127 bp region from -595 to -468 bp is critical for combinatorial regulation by light with sugar or gibberellin signals.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Mechanisms
signal integration of light with gibberellin signalingsignal integration of light with sugar signalingsugar-dependent transcriptional repression in the darktranscriptional regulation by promoter cis-elementsTechniques
Computational DesignTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: reporter
The source study isolated an 895 bp RhVI1 promoter fragment and tested shorter fragments by reporter gene assays. A 468 bp fragment retained responsiveness to light, sugars, and gibberellins, while the -595 to -468 bp region was required for specific synergistic regulation. The promoter originates from Rosa hybrida, and endogenous RhVI1 expression was reported in epidermal cells of young leaves of bursting buds.
The available evidence comes from a single 2014 study and does not establish independent replication. Quantitative performance characteristics, such as fold induction, basal activity, kinetics, and cross-species portability, are not provided in the supplied evidence. The evidence supports promoter responsiveness and mapping, but not sequence-resolved motif function beyond the defined promoter intervals.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The 127 bp promoter fragment between -595 and -468 bp is critical for synergistic action of light with sugar and of light with gibberellins.
These results reveal that the 127 bp promoter fragment located between -595 and -468 bp is critical for light and sugar and light and gibberellins to act synergistically.
critical fragment length 127 bp
The 127 bp promoter fragment between -595 and -468 bp is critical for synergistic action of light with sugar and of light with gibberellins.
These results reveal that the 127 bp promoter fragment located between -595 and -468 bp is critical for light and sugar and light and gibberellins to act synergistically.
critical fragment length 127 bp
The 127 bp promoter fragment between -595 and -468 bp is critical for synergistic action of light with sugar and of light with gibberellins.
These results reveal that the 127 bp promoter fragment located between -595 and -468 bp is critical for light and sugar and light and gibberellins to act synergistically.
critical fragment length 127 bp
The 127 bp promoter fragment between -595 and -468 bp is critical for synergistic action of light with sugar and of light with gibberellins.
These results reveal that the 127 bp promoter fragment located between -595 and -468 bp is critical for light and sugar and light and gibberellins to act synergistically.
critical fragment length 127 bp
The 127 bp promoter fragment between -595 and -468 bp is critical for synergistic action of light with sugar and of light with gibberellins.
These results reveal that the 127 bp promoter fragment located between -595 and -468 bp is critical for light and sugar and light and gibberellins to act synergistically.
critical fragment length 127 bp
The 127 bp promoter fragment between -595 and -468 bp is critical for synergistic action of light with sugar and of light with gibberellins.
These results reveal that the 127 bp promoter fragment located between -595 and -468 bp is critical for light and sugar and light and gibberellins to act synergistically.
critical fragment length 127 bp
The 127 bp promoter fragment between -595 and -468 bp is critical for synergistic action of light with sugar and of light with gibberellins.
These results reveal that the 127 bp promoter fragment located between -595 and -468 bp is critical for light and sugar and light and gibberellins to act synergistically.
critical fragment length 127 bp
A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
promoter fragment length 468 bp
A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
promoter fragment length 468 bp
A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
promoter fragment length 468 bp
A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
promoter fragment length 468 bp
A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
promoter fragment length 468 bp
A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
promoter fragment length 468 bp
A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
promoter fragment length 468 bp
RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
The -595 to -468 bp RhVI1 promoter region carrying a sugar-repressive element is required to down-regulate the promoter in response to sucrose and fructose in the dark.
the -595 to -468 bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark.
The -595 to -468 bp RhVI1 promoter region carrying a sugar-repressive element is required to down-regulate the promoter in response to sucrose and fructose in the dark.
the -595 to -468 bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark.
The -595 to -468 bp RhVI1 promoter region carrying a sugar-repressive element is required to down-regulate the promoter in response to sucrose and fructose in the dark.
the -595 to -468 bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark.
The -595 to -468 bp RhVI1 promoter region carrying a sugar-repressive element is required to down-regulate the promoter in response to sucrose and fructose in the dark.
the -595 to -468 bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark.
The -595 to -468 bp RhVI1 promoter region carrying a sugar-repressive element is required to down-regulate the promoter in response to sucrose and fructose in the dark.
the -595 to -468 bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark.
The -595 to -468 bp RhVI1 promoter region carrying a sugar-repressive element is required to down-regulate the promoter in response to sucrose and fructose in the dark.
the -595 to -468 bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark.
The -595 to -468 bp RhVI1 promoter region carrying a sugar-repressive element is required to down-regulate the promoter in response to sucrose and fructose in the dark.
the -595 to -468 bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark.
In silico analysis identified putative cis-elements involved in response to sugars, light, and gibberellins in the proximal 595 bp part of the RhVI1 promoter.
In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595 bp).
proximal promoter length 595 bp
In silico analysis identified putative cis-elements involved in response to sugars, light, and gibberellins in the proximal 595 bp part of the RhVI1 promoter.
In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595 bp).
proximal promoter length 595 bp
In silico analysis identified putative cis-elements involved in response to sugars, light, and gibberellins in the proximal 595 bp part of the RhVI1 promoter.
In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595 bp).
proximal promoter length 595 bp
In silico analysis identified putative cis-elements involved in response to sugars, light, and gibberellins in the proximal 595 bp part of the RhVI1 promoter.
In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595 bp).
proximal promoter length 595 bp
In silico analysis identified putative cis-elements involved in response to sugars, light, and gibberellins in the proximal 595 bp part of the RhVI1 promoter.
In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595 bp).
proximal promoter length 595 bp
In silico analysis identified putative cis-elements involved in response to sugars, light, and gibberellins in the proximal 595 bp part of the RhVI1 promoter.
In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595 bp).
proximal promoter length 595 bp
In silico analysis identified putative cis-elements involved in response to sugars, light, and gibberellins in the proximal 595 bp part of the RhVI1 promoter.
In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595 bp).
proximal promoter length 595 bp
The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.
This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.
The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.
This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.
The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.
This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.
The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.
This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.
The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.
This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.
The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.
This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.
The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.
This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.
Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.
We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.
promoter fragment length 595 bp
Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.
We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.
promoter fragment length 595 bp
Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.
We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.
promoter fragment length 595 bp
Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.
We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.
promoter fragment length 595 bp
Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.
We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.
promoter fragment length 595 bp
Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.
We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.
promoter fragment length 595 bp
Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.
We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.
promoter fragment length 595 bp
Approval Evidence
1 source7 linked approval claimsfirst-pass slug rhvi1-promoter
We then isolated a 895 bp fragment of the promoter of RhVI1.
Source:
critical regionsupports
The 127 bp promoter fragment between -595 and -468 bp is critical for synergistic action of light with sugar and of light with gibberellins.
These results reveal that the 127 bp promoter fragment located between -595 and -468 bp is critical for light and sugar and light and gibberellins to act synergistically.
Source:
functional sufficiencysupports
A 468 bp RhVI1 promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars, and gibberellins.
Deletion analysis revealed that the 468 bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins.
Source:
localizationsupports
RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaves of bursting buds.
Source:
required regionsupports
The -595 to -468 bp RhVI1 promoter region carrying a sugar-repressive element is required to down-regulate the promoter in response to sucrose and fructose in the dark.
the -595 to -468 bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark.
Source:
sequence featuresupports
In silico analysis identified putative cis-elements involved in response to sugars, light, and gibberellins in the proximal 595 bp part of the RhVI1 promoter.
In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595 bp).
Source:
stimulus specific responsesupports
The 468 bp RhVI1 promoter region confers sucrose- and fructose-responsive, but not glucose-responsive, activation in the dark.
This region confers sucrose- and fructose-, but not glucose-, responsive activation in the dark.
Source:
synergistic regulationsupports
Sugar plus light and gibberellin plus light act synergistically to sharply up-regulate GUS activity under control of the 595 bp pRhVI1 region.
We also demonstrate that sugar/light and gibberellin/light act synergistically to up-regulate β-glucuronidase (GUS) activity sharply under the control of the 595 bp pRhVI1 region.
Source:
Comparisons
Source-backed strengths
Evidence indicates that a relatively short 468 bp fragment is sufficient to drive reporter activity in response to light, sugars, and gibberellins, supporting functional compactness. A 127 bp segment was further identified as critical for synergistic action of light with sugar and of light with gibberellins, giving the promoter a mapped regulatory core. RhVI1 expression was also localized to epidermal cells of young leaves of bursting buds.
Compared with phosphorothioate-caged antisense oligonucleotides
RhVI1 promoter and phosphorothioate-caged antisense oligonucleotides address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with photo-sensitive circular gRNAs
RhVI1 promoter and photo-sensitive circular gRNAs address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with RNA aptamer
RhVI1 promoter and RNA aptamer address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.