Source 1primary paper2017Nanophotonics
Toolkit/single-molecule fluorescence measurements
single-molecule fluorescence measurements
Also known as: single-molecule fluorescence
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
In this review we provide an overview of single-molecule fluorescence measurements on plant light-harvesting complexes (LHCs) of varying sizes with the aim of bridging the gap between the smallest isolated complexes, which have been well-characterized, and the native photosystem.
Usefulness & Problems
Why this is useful
Single-molecule fluorescence measurements are used to examine plant light-harvesting complexes across different organizational scales. The review presents them as a way to connect well-characterized isolated complexes to native photosystems.; probing plant light-harvesting complexes of varying sizes; bridging observations from isolated complexes to native photosystems; studying conformational dynamics in light-harvesting complexes
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Single-molecule fluorescence measurements are used to examine plant light-harvesting complexes across different organizational scales. The review presents them as a way to connect well-characterized isolated complexes to native photosystems.
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probing plant light-harvesting complexes of varying sizes
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bridging observations from isolated complexes to native photosystems
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studying conformational dynamics in light-harvesting complexes
Problem solved
It helps resolve how properties observed in small isolated complexes relate to the behavior of the native thylakoid photosystem. It also supports analysis of conformational dynamics and environment-dependent function.; helps connect measurements on small isolated complexes to behavior in larger native photosystems
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It helps resolve how properties observed in small isolated complexes relate to the behavior of the native thylakoid photosystem. It also supports analysis of conformational dynamics and environment-dependent function.
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helps connect measurements on small isolated complexes to behavior in larger native photosystems
Problem links
helps connect measurements on small isolated complexes to behavior in larger native photosystems
LiteratureIt helps resolve how properties observed in small isolated complexes relate to the behavior of the native thylakoid photosystem. It also supports analysis of conformational dynamics and environment-dependent function.
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It helps resolve how properties observed in small isolated complexes relate to the behavior of the native thylakoid photosystem. It also supports analysis of conformational dynamics and environment-dependent function.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
fluorescence measurementTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The method requires fluorescence measurements on plant light-harvesting complexes, including isolated complexes and larger native-like assemblies. The abstract also implies the need for systems spanning different sizes and environments.; requires fluorescence measurement capability; requires plant light-harvesting complex samples of varying sizes
The abstract does not show that single-molecule fluorescence alone fully explains in vivo photosystem behavior. Larger in vivo systems are described as a later testing context for bottom-up models.; the abstract does not specify exact assay formats or throughput; the review frames the method as part of a bridge to larger in vivo systems rather than a complete in vivo solution
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Single-molecule fluorescence measurements on plant light-harvesting complexes can bridge understanding between the smallest isolated complexes and the native photosystem.
Experimental results from these studies can be used to build bottom-up mathematical-physical models that can then be tested on larger in vivo systems.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug single-molecule-fluorescence-measurements
In this review we provide an overview of single-molecule fluorescence measurements on plant light-harvesting complexes (LHCs) of varying sizes with the aim of bridging the gap between the smallest isolated complexes, which have been well-characterized, and the native photosystem.
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method usesupports
Single-molecule fluorescence measurements on plant light-harvesting complexes can bridge understanding between the smallest isolated complexes and the native photosystem.
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modeling usesupports
Experimental results from these studies can be used to build bottom-up mathematical-physical models that can then be tested on larger in vivo systems.
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Comparisons
Source-stated alternatives
The abstract does not explicitly name alternative measurement methods. It contrasts isolated-complex studies with testing on larger in vivo systems.
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The abstract does not explicitly name alternative measurement methods. It contrasts isolated-complex studies with testing on larger in vivo systems.
Source-backed strengths
supports single-molecule-level analysis of heterogeneous light-harvesting complexes; is presented as useful for building bottom-up mathematical-physical models
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supports single-molecule-level analysis of heterogeneous light-harvesting complexes
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is presented as useful for building bottom-up mathematical-physical models
Compared with CLARITY technology
single-molecule fluorescence measurements and CLARITY technology address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with Langendorff perfused heart electrical recordings
single-molecule fluorescence measurements and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Compared with native green gel system
single-molecule fluorescence measurements and native green gel system address a similar problem space.
Shared frame: same top-level item type; same primary input modality: light
Ranked Citations
- 1.