Source 1primary paper2021Journal of Visualized Experiments
Toolkit/TAEL 2.0
TAEL 2.0
Also known as: TAEL, TAEL/C120 system
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
TAEL 2.0 is a modified version of the TAEL/C120 optogenetic transcription system in which both the TAEL transcriptional activator and the C120 regulatory element were altered. In zebrafish embryos, blue light induces TAEL dimerization, binding to the C120 element, and transcriptional activation to drive light-inducible gene expression.
Usefulness & Problems
Why this is useful
This system is useful for optically controlling gene expression in zebrafish embryos with blue light. The available evidence supports its use as a light-inducible transcription switch based on the TAEL activator and C120 regulatory sequence.
Source:
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
Source:
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
Problem solved
TAEL 2.0 addresses the need for inducible gene expression in zebrafish embryos using a light-responsive regulatory system. The evidence specifically supports blue-light-triggered activation of transcription through the TAEL/C120 module.
Source:
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
Source:
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
Problem links
Need conditional recombination or state switching
DerivedTAEL 2.0 is a modified version of the TAEL/C120 optogenetic transcription system, comprising changes to both the TAEL transcriptional activator and the C120 regulatory element. In zebrafish embryos, blue light induces TAEL dimerization, C120 binding, and transcriptional activation, enabling light-inducible gene expression.
Need tighter control over gene expression timing or amplitude
DerivedTAEL 2.0 is a modified version of the TAEL/C120 optogenetic transcription system, comprising changes to both the TAEL transcriptional activator and the C120 regulatory element. In zebrafish embryos, blue light induces TAEL dimerization, C120 binding, and transcriptional activation, enabling light-inducible gene expression.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A composed arrangement of multiple parts that instantiates one or more mechanisms.
Mechanisms
dna binding to the c120 regulatory elementdna binding to the c120 regulatory elementlight-induced dimerizationlight-induced dimerizationtranscriptional activationtranscriptional activationTechniques
No technique tags yet.
Target processes
recombinationtranscriptionImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: multi component delivery burdenoperating role: sensorswitch architecture: multi component
TAEL 2.0 comprises engineered changes to both the TAEL transcriptional activator and the C120 regulatory element. The documented application is in zebrafish embryos under blue-light illumination, but the provided evidence does not specify construct architecture, promoter context, delivery method, or cofactor requirements.
The supplied evidence does not report quantitative performance metrics, dynamic range, background activity, kinetics, or comparisons between TAEL 2.0 and the original TAEL/C120 system. Validation is only described for zebrafish embryos in the provided material.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Source 2primary paper2020
Ranked Claims
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
peak GFP induction 130 foldtime to first detection of GFP induction 30 min
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
A ubiquitous TAEL 2.0 transgenic line can induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
The improvements in TAEL 2.0 enabled creation of functional stable transgenic lines expressing the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
TAEL 2.0 induces higher levels of reporter gene expression and does so faster than the original TAEL system, while maintaining comparable background and toxicity.
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
Approval Evidence
2 sources7 linked approval claimsfirst-pass slugs tael-2-0, tael-c120-system
the Optogenetic TAEL/C120 System
Source:
We made modifications to both the TAEL transcriptional activator and the C120 regulatory element, collectively referred to as TAEL 2.0.
Source:
applicationsupports
The TAEL/C120 system is used to achieve light-inducible gene expression in zebrafish embryos.
In this protocol, an optogenetic expression system is used to achieve light-inducible gene expression in zebrafish embryos.
Source:
mechanismsupports
Blue light causes TAEL to dimerize, bind C120, and activate transcription.
When illuminated with blue light, TAEL dimerizes, binds to its cognate regulatory element called C120, and activates transcription.
Source:
performancesupports
Blue-light illumination induces GFP expression detectable after 30 minutes and reaching more than 130-fold induction after 3 hours in transgenic zebrafish embryos using the TAEL/C120 system.
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
Source:
usabilitysupports
The method is described as a versatile and easy-to-use approach for optogenetic gene expression.
This method is a versatile and easy-to-use approach for optogenetic gene expression.
Source:
application scopesupports
A ubiquitous TAEL 2.0 transgenic line can induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
We demonstrate that the ubiquitous line in particular can be used to induce expression at late embryonic and larval stages, addressing a major deficiency of the original TAEL system.
Source:
engineering outcomesupports
The improvements in TAEL 2.0 enabled creation of functional stable transgenic lines expressing the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
Source:
performance improvementsupports
TAEL 2.0 induces higher levels of reporter gene expression and does so faster than the original TAEL system, while maintaining comparable background and toxicity.
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
Source:
Comparisons
Source-backed strengths
The system has a defined optogenetic mechanism in which blue light causes TAEL dimerization, C120 binding, and transcriptional activation. It is directly reported for light-induced gene expression in zebrafish embryos, and TAEL 2.0 includes modifications to both the activator and regulatory element.
Source:
induction of GFP expression can first be detected after 30 min of illumination and reaches a peak of more than 130-fold induction after 3 h of light treatment
Source:
With these improvements, we were able to create functional stable transgenic lines to express the TAEL 2.0 transcription factor either ubiquitously or with a tissue-specific promoter.
Source:
We demonstrate that TAEL 2.0 consistently induces higher levels of reporter gene expression and at a faster rate, but with comparable background and toxicity as the original TAEL system.
Compared with FUN-LOV
TAEL 2.0 and FUN-LOV address a similar problem space because they share recombination, transcription.
Shared frame: same top-level item type; shared target processes: recombination, transcription; shared mechanisms: transcriptional activation
Relative tradeoffs: appears more independently replicated; looks easier to implement in practice.
Compared with Opto-T7RNAP
TAEL 2.0 and Opto-T7RNAP address a similar problem space because they share recombination, transcription.
Shared frame: same top-level item type; shared target processes: recombination, transcription; shared mechanisms: transcriptional activation
Strengths here: looks easier to implement in practice.
Compared with synthetic optogenetic transcription device
TAEL 2.0 and synthetic optogenetic transcription device address a similar problem space because they share recombination, transcription.
Shared frame: same top-level item type; shared target processes: recombination, transcription; shared mechanisms: transcriptional activation
Relative tradeoffs: appears more independently replicated.
Ranked Citations
- 1.