Source 1primary paper1996Genome Research
Toolkit/TaqMan probe
TaqMan probe
Also known as: dual-labeled fluorogenic probe
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe).
Usefulness & Problems
Why this is useful
The TaqMan probe is the dual-labeled fluorogenic probe used to measure PCR product accumulation in the reported real-time quantitative PCR method.; measuring PCR product accumulation during real-time quantitative PCR
Source:
The TaqMan probe is the dual-labeled fluorogenic probe used to measure PCR product accumulation in the reported real-time quantitative PCR method.
Source:
measuring PCR product accumulation during real-time quantitative PCR
Problem solved
It provides the signal readout that allows PCR product accumulation to be monitored during the assay.; enables fluorescence-based monitoring of PCR product accumulation
Source:
It provides the signal readout that allows PCR product accumulation to be monitored during the assay.
Source:
enables fluorescence-based monitoring of PCR product accumulation
Problem links
enables fluorescence-based monitoring of PCR product accumulation
LiteratureIt provides the signal readout that allows PCR product accumulation to be monitored during the assay.
Source:
It provides the signal readout that allows PCR product accumulation to be monitored during the assay.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
It requires incorporation into the PCR assay as the fluorogenic detection component.; must be used as a dual-labeled fluorogenic probe within the PCR assay
Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Compared with other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, which helps prevent PCR product carry-over contamination and makes assays faster and higher throughput.
Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays.
Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
Real-time quantitative PCR has a dynamic range for starting target molecule determination of at least five orders of magnitude.
The real-time PCR method has a very large dynamic range of starting target molecule determination (at least five orders of magnitude).
dynamic range 5 orders_of_magnitude
Real-time quantitative PCR measures PCR product accumulation through a dual-labeled fluorogenic probe identified as the TaqMan probe.
The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe).
The paper introduces a novel real-time quantitative PCR method.
We have developed a novel "real time" quantitative PCR method.
The real-time quantitative PCR method provides very accurate and reproducible quantitation of gene copies.
This method provides very accurate and reproducible quantitation of gene copies.
accuracy very accuratereproducibility reproducible
Approval Evidence
1 source1 linked approval claimfirst-pass slug taqman-probe
The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe).
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mechanismsupports
Real-time quantitative PCR measures PCR product accumulation through a dual-labeled fluorogenic probe identified as the TaqMan probe.
The method measures PCR product accumulation through a dual-labeled fluorogenic probe (i.e., TaqMan Probe).
Source:
Comparisons
Source-backed strengths
Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays. Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods. This method provides very accurate and reproducible quantitation of gene copies.
Source:
Unlike other quantitative PCR methods, real-time PCR does not require post-PCR sample handling, preventing potential PCR product carry-over contamination and resulting in much faster and higher throughput assays.
Source:
Real-time quantitative PCR is extremely accurate and less labor-intensive than current quantitative PCR methods.
Source:
This method provides very accurate and reproducible quantitation of gene copies.
Compared with Langendorff perfused heart electrical recordings
TaqMan probe and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with native green gel system
TaqMan probe and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
TaqMan probe and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.