Source 1primary paper2014Photochemical & Photobiological Sciences
Toolkit/two-dimensional transient absorption
two-dimensional transient absorption
Also known as: 2D-TA
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Two-dimensional transient absorption (2D-TA) is a transient absorption spectroscopy assay used with a streak camera to search for short-lived photochemical intermediates in LOV domain photocycles. In the cited study, it was applied extensively and did not detect an intermediate with a rate constant between fluorescence decay and triplet-state decay.
Usefulness & Problems
Why this is useful
This method is useful for interrogating whether transient photochemical intermediates arise during LOV domain photocycling. The cited evidence supports its use as a negative-screening assay for intermediates in a kinetic window bounded by fluorescence decay and triplet-state decay.
Problem solved
2D-TA addresses the problem of testing for elusive radical or other transient intermediates in LOV domain photocycles. In the cited work, it was specifically used to search for an intermediate with kinetics between fluorescence decay and triplet-state decay.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The assay was implemented as two-dimensional transient absorption with a streak camera. The available evidence does not specify construct design, cofactors, illumination conditions, or sample preparation parameters.
The cited evidence reports non-detection of a transient intermediate rather than positive identification of a species. No details are provided here on sensitivity, spectral range, temporal resolution, sample requirements, or performance outside LOV domains.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Approval Evidence
1 source1 linked approval claimfirst-pass slug two-dimensional-transient-absorption
We performed an extensive search for these intermediates by two-dimensional transient absorption (2D-TA) with a streak camera.
Source:
negative detectionsupports
Two-dimensional transient absorption did not detect a transient intermediate with a rate constant between fluorescence decay and triplet-state decay in LOV domains.
However, no transient with a rate constant between the decay of fluorescence and the decay of the triplet state could be detected.
Source:
Comparisons
Source-backed strengths
The method was used in an extensive search for intermediates in LOV domains, indicating suitability for time-resolved photochemical interrogation. The available evidence only supports a negative finding in this application and does not provide broader performance metrics.
Compared with Field-domain rapid-scan EPR at 240 GHz
two-dimensional transient absorption and Field-domain rapid-scan EPR at 240 GHz address a similar problem space.
Shared frame: same top-level item type
Compared with fluorescence line narrowing
two-dimensional transient absorption and fluorescence line narrowing address a similar problem space.
Shared frame: same top-level item type
Compared with native green gel system
two-dimensional transient absorption and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.