Source 1primary paper2016Scientific Reports
Toolkit/ultrafast wide-field macroscope for optical mapping
ultrafast wide-field macroscope for optical mapping
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
An ultrafast wide-field macroscope was developed to optically map action potential propagation with a red-shifted voltage sensitive dye in whole mouse hearts.
Usefulness & Problems
Why this is useful
This macroscope optically maps action potential propagation across whole mouse hearts. In the abstract it serves as the measurement arm of the all-optical platform.; optical mapping of action potential propagation in whole mouse hearts
Source:
This macroscope optically maps action potential propagation across whole mouse hearts. In the abstract it serves as the measurement arm of the all-optical platform.
Source:
optical mapping of action potential propagation in whole mouse hearts
Problem solved
It provides the propagation readout needed to assess arrhythmia dynamics and the effects of patterned optical stimulation.; provides whole-heart optical readout needed to evaluate stimulation pattern effects
Source:
It provides the propagation readout needed to assess arrhythmia dynamics and the effects of patterned optical stimulation.
Source:
provides whole-heart optical readout needed to evaluate stimulation pattern effects
Problem links
provides whole-heart optical readout needed to evaluate stimulation pattern effects
LiteratureIt provides the propagation readout needed to assess arrhythmia dynamics and the effects of patterned optical stimulation.
Source:
It provides the propagation readout needed to assess arrhythmia dynamics and the effects of patterned optical stimulation.
Published Workflows
Objective: Develop and use an all-optical platform to design less invasive, mechanistically based cardiac defibrillation strategies in whole mouse hearts.
Why it works: The workflow combines whole-heart optical mapping with arbitrary sub-millisecond patterned stimulation so that arrhythmia propagation can be observed and then interrupted using mechanistically designed multi-barrier patterns rather than generalized whole-ventricle discharge.
creation of multiple regions of conduction blockprecise epicardial activation of ChR2optical mappingpatterned optogenetic stimulationmechanistic stimulation pattern optimization
Stages
- 1.whole-heart optical mapping platform setup(functional_characterization)
This stage provides the propagation information needed to examine and design less invasive defibrillation strategies.
Selection: Acquire action potential propagation maps in whole mouse hearts using an ultrafast wide-field macroscope and red-shifted voltage sensitive dye.
- 2.patterned optogenetic stimulation(functional_characterization)
This stage enables testing of discrete, mechanistically designed stimulation patterns rather than generalized whole-ventricle interventions.
Selection: Use a random-access scanning head to draw arbitrarily chosen stimulation patterns with sub-millisecond temporal resolution for precise epicardial ChR2 activation.
- 3.ventricular tachycardia pattern optimization and cardioversion assessment(confirmatory_validation)
This stage tests whether discrete stimulation patterns can interrupt arrhythmias efficiently while reducing required energy.
Selection: Evaluate mechanistic multi-barrier cardioversion/defibrillation patterns in ventricular tachycardia based on cardioversion efficiency and lower energy requirements relative to whole ventricle interventions.
Steps
- 1.Map action potential propagation in whole mouse heartsmeasurement platform
Obtain optical readout of action potential propagation in whole mouse hearts.
Propagation mapping is needed before mechanistic stimulation patterns can be examined and optimized.
- 2.Deliver arbitrarily chosen sub-millisecond stimulation patterns for epicardial ChR2 activationstimulation hardware
Apply precise patterned optical stimulation to the epicardium.
After mapping, patterned stimulation can be used to test mechanistic interventions against the observed arrhythmia substrate.
- 3.Optimize mechanistic multi-barrier cardioversion/defibrillation patterns in ventricular tachycardiaintegrated all-optical platform
Identify stimulation patterns that interrupt arrhythmias efficiently with lower energy than whole ventricle interventions.
Optimization follows once the system can both map propagation and deliver precise patterned stimulation.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract explicitly states that mapping was performed with a red-shifted voltage sensitive dye in whole mouse hearts.; requires a red-shifted voltage sensitive dye; used in whole mouse hearts
abstract does not report quantitative mapping performance metrics
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
In ventricular tachycardia, mechanistic multi-barrier cardioversion/defibrillation patterns created multiple regions of conduction block with very high cardioversion efficiency and lower energy requirements than whole ventricle interventions.
Multiple regions of conduction block were created with a very high cardioversion efficiency but with lower energy requirements as compared to whole ventricle interventions to interrupt arrhythmias.
cardioversion efficiency very high
An ultrafast wide-field macroscope was developed to optically map action potential propagation with a red-shifted voltage sensitive dye in whole mouse hearts.
An ultrafast wide-field macroscope was developed to optically map action potential propagation with a red-shifted voltage sensitive dye in whole mouse hearts.
The random-access scanning head can draw arbitrarily chosen stimulation patterns with sub-millisecond temporal resolution for precise epicardial activation of ChR2.
The macroscope was implemented with a random-access scanning head capable of drawing arbitrarily-chosen stimulation patterns with sub-millisecond temporal resolution allowing precise epicardial activation of Channelrhodopsin2 (ChR2).
temporal resolution sub-millisecond
Applying discrete stimulation patterns can substantially reduce defibrillation energies.
This work demonstrates that defibrillation energies can be substantially reduced by applying discrete stimulation patterns and promotes the progress of current anti-arrhythmic strategies.
The study developed an all-optical platform to examine less invasive defibrillation strategies.
Here, we developed an all-optical platform to examine less invasive defibrillation strategies.
Approval Evidence
1 source1 linked approval claimfirst-pass slug ultrafast-wide-field-macroscope-for-optical-mapping
An ultrafast wide-field macroscope was developed to optically map action potential propagation with a red-shifted voltage sensitive dye in whole mouse hearts.
Source:
capabilitysupports
An ultrafast wide-field macroscope was developed to optically map action potential propagation with a red-shifted voltage sensitive dye in whole mouse hearts.
An ultrafast wide-field macroscope was developed to optically map action potential propagation with a red-shifted voltage sensitive dye in whole mouse hearts.
Source:
Comparisons
Source-stated alternatives
The abstract does not name a direct alternative mapping instrument, but it contrasts the overall strategy with generalized electrical rescue approaches.
Source:
The abstract does not name a direct alternative mapping instrument, but it contrasts the overall strategy with generalized electrical rescue approaches.
Source-backed strengths
ultrafast; wide-field
Source:
ultrafast
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wide-field
Compared with Langendorff perfused heart electrical recordings
ultrafast wide-field macroscope for optical mapping and Langendorff perfused heart electrical recordings address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Compared with native green gel system
ultrafast wide-field macroscope for optical mapping and native green gel system address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
ultrafast wide-field macroscope for optical mapping and sub-picosecond pump-probe analysis of bacteriorhodopsin pigments address a similar problem space.
Shared frame: same top-level item type
Strengths here: looks easier to implement in practice.
Ranked Citations
- 1.