Source 1primary paper2013Frontiers in Neuroscience
Toolkit/voltage-sensitive dye imaging
voltage-sensitive dye imaging
Also known as: VSD imaging, VSDs
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
We review recently developed functional mapping methods that use optogenetic single-point stimulation in the rodent brain and employ cellular electrophysiology, evoked motor movements, voltage sensitive dyes (VSDs), calcium indicators, or functional magnetic resonance imaging (fMRI) to assess activity.
Usefulness & Problems
Why this is useful
Voltage-sensitive dye imaging reports activity patterns during optogenetic stimulation for functional brain mapping. The abstract specifically highlights red-shifted organic VSDs as a high-temporal-resolution readout.; assessing activity during optogenetic functional mapping; high temporal resolution cortical mapping
Source:
Voltage-sensitive dye imaging reports activity patterns during optogenetic stimulation for functional brain mapping. The abstract specifically highlights red-shifted organic VSDs as a high-temporal-resolution readout.
Source:
assessing activity during optogenetic functional mapping
Source:
high temporal resolution cortical mapping
Problem solved
It enables optical mapping of stimulated activity with high temporal resolution and can reveal circuit structure related to sensory processing.; provides an optical readout of activity during optogenetic stimulation
Source:
It enables optical mapping of stimulated activity with high temporal resolution and can reveal circuit structure related to sensory processing.
Source:
provides an optical readout of activity during optogenetic stimulation
Problem links
We Can’t Take High-Resolution Movies of or Intervene in Brain Computation at the Single Neuron Level
Gap mapView gapVoltage-sensitive dye imaging is at least directly an activity-readout method and the supplied evidence places it in rodent brain functional mapping, which is closer to the gap context than most other items. Its high temporal-resolution hint is relevant to capturing fast computation, even though the provided evidence does not establish single-neuron resolution.
provides an optical readout of activity during optogenetic stimulation
LiteratureIt enables optical mapping of stimulated activity with high temporal resolution and can reveal circuit structure related to sensory processing.
Source:
It enables optical mapping of stimulated activity with high temporal resolution and can reveal circuit structure related to sensory processing.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
intracortical synaptic activity readoutoptogenetic photoactivationvoltage-dependent optical reportingTechniques
Functional AssayTarget processes
No target processes tagged yet.
Input: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: sensor
The method requires voltage-sensitive dyes and optical imaging configured to read signals during optogenetic stimulation. For the highlighted use case, spectral separation from ChR2 activation is required.; requires compatible dye and optical setup; spectral separation from the stimulation opsin is important
The abstract notes that challenges remain for selective, high-temporal-resolution assessment in animals that can be followed longitudinally.; longitudinal selective high temporal resolution assessment in animals remains challenging
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Optogenetic stimulation allows arbitrary stimulation of opsin-expressing brain regions, enabling brain mapping independent of behavior or sensory processing.
Red-shifted organic voltage-sensitive dyes permit high temporal resolution imaging that is spectrally separated from Channelrhodopsin-2 activation.
VSD maps stimulated by ChR2 were dependent on intracortical synaptic activity and reflected circuits used for sensory processing.
Opsin-based activation allows investigation of connectivity with spatial resolution on the order of single neurons and temporal resolution on the order of milliseconds.
spatial resolution on the order of single neuronstemporal resolution on the order of milliseconds
Approval Evidence
1 source2 linked approval claimsfirst-pass slug voltage-sensitive-dye-imaging
We review recently developed functional mapping methods that use optogenetic single-point stimulation in the rodent brain and employ cellular electrophysiology, evoked motor movements, voltage sensitive dyes (VSDs), calcium indicators, or functional magnetic resonance imaging (fMRI) to assess activity.
Source:
compatibilitysupports
Red-shifted organic voltage-sensitive dyes permit high temporal resolution imaging that is spectrally separated from Channelrhodopsin-2 activation.
Source:
mechanistic observationsupports
VSD maps stimulated by ChR2 were dependent on intracortical synaptic activity and reflected circuits used for sensory processing.
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Comparisons
Source-stated alternatives
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Source:
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Source-backed strengths
can provide high temporal resolution imaging; red-shifted organic VSDs can be spectrally separated from ChR2 activation
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can provide high temporal resolution imaging
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red-shifted organic VSDs can be spectrally separated from ChR2 activation
Compared with calcium indicators
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Shared frame: source-stated alternative in extracted literature
Strengths here: can provide high temporal resolution imaging; red-shifted organic VSDs can be spectrally separated from ChR2 activation.
Relative tradeoffs: longitudinal selective high temporal resolution assessment in animals remains challenging.
Source:
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Compared with electrophysiology
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Shared frame: source-stated alternative in extracted literature
Strengths here: can provide high temporal resolution imaging; red-shifted organic VSDs can be spectrally separated from ChR2 activation.
Relative tradeoffs: longitudinal selective high temporal resolution assessment in animals remains challenging.
Source:
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Compared with functional magnetic resonance imaging
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Shared frame: source-stated alternative in extracted literature
Strengths here: can provide high temporal resolution imaging; red-shifted organic VSDs can be spectrally separated from ChR2 activation.
Relative tradeoffs: longitudinal selective high temporal resolution assessment in animals remains challenging.
Source:
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Compared with imaging
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Shared frame: source-stated alternative in extracted literature
Strengths here: can provide high temporal resolution imaging; red-shifted organic VSDs can be spectrally separated from ChR2 activation.
Relative tradeoffs: longitudinal selective high temporal resolution assessment in animals remains challenging.
Source:
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Compared with imaging surveillance
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Shared frame: source-stated alternative in extracted literature
Strengths here: can provide high temporal resolution imaging; red-shifted organic VSDs can be spectrally separated from ChR2 activation.
Relative tradeoffs: longitudinal selective high temporal resolution assessment in animals remains challenging.
Source:
The abstract contrasts VSD imaging with cellular electrophysiology, evoked motor movements, calcium indicators, and fMRI as other activity readouts.
Ranked Citations
- 1.