Browse the toolkit beneath workflows. The mechanism branch runs mechanism -> architecture -> component, while the technique branch runs from high-level approaches down to concrete methods.
8 items matching 1 filter
Mechanism Branch
Layer 1
Mechanisms
Top-level concepts: biophysical action modes such as heterodimerization, photocleavage, or RNA binding.
Layer 2
Architectures
Arrangements that realize or deploy mechanisms, including switches, construct patterns, and delivery strategies.
Layer 3
Components
Low-level parts and sequence-defined elements used inside architectures, including protein domains and RNA elements.
Technique Branch
Layer 1
Approaches
High-level engineering practices such as computational design, directed evolution, sequence verification, and functional assay.
Layer 2
Methods
Concrete methods used to design, build, verify, or characterize engineered systems.
Showing 1-8 of 8
The Cry2-METTL3 fusion construct is an optogenetic domain-fusion reporter in which METTL3 is fused to Cry2 to track components of the mRNA m6A methyltransferase complex. In the cited study, it was used to monitor assembly behavior associated with METTL3 phase separation.
Here we show an imaging-based method that profiles protein-protein interactions (PPIs) through phase-separated condensates.
Biomolecular liquid-liquid phase separation (LLPS) is a cellular organizational phenomenon in which biomolecules demix into condensed phases. The cited review describes LLPS as contributing to cellular homeostasis and specifically to cellular redox maintenance, including redox imbalance sensing, signal transduction, and transcriptional regulation.
Opto-controlled C9orf72 poly-PR is a light-responsive cellular construct used to induce poly-PR condensation and aggregation in cultured cells with spatial and temporal control. In the reported 2024 study, it generated anisotropic poly-PR condensates associated with nuclear TDP-43 granulation and pathology-relevant phenotypes.
CluMPS (Clusters Magnified by Phase Separation) is a fluorescent reporter strategy for high-sensitivity detection of protein clusters in cells. It is reported to visualize submicroscopic clusters, including small aggregates, and to track clusters of unmodified, tagged, and endogenous proteins.
OptoDroplet is an optogenetic multi-component switch that controls biomolecular phase separation by fusing disease-associated proteins to light-sensitive oligomerization domains. Light input enables induction or reversal of condensate formation with spatial and temporal control.
This method involves air-induced, temperature-controlled phase separation (AITCPS), which enables the formation of highly dense PLA matrices.
Explicitly supported tool/component names recovered from sources include optoDroplets, Corelets, OptoGranules, Cry2, iLID, SspB, ferritin/FTH1, G3BP1, and MBP-based OptoMBP.