Source 1review2019Current Opinion in Structural Biology
Toolkit/bacteriophytochrome-derived fluorescent probes
bacteriophytochrome-derived fluorescent probes
Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Considering their preferential interaction with biliverdin IXα as endogenous cofactor, they have recently been used for creating optogenetic tools and engineering fluorescent probes.
Usefulness & Problems
Why this is useful
These are fluorescent probes engineered from bacteriophytochrome scaffolds. The abstract presents them as a major application area enabled by bacteriophytochrome biliverdin binding.; engineering fluorescent probes; near-infrared imaging-related probe development
Source:
These are fluorescent probes engineered from bacteriophytochrome scaffolds. The abstract presents them as a major application area enabled by bacteriophytochrome biliverdin binding.
Source:
engineering fluorescent probes
Source:
near-infrared imaging-related probe development
Problem solved
They provide a route to fluorescent probe engineering using photoreceptor scaffolds suited to near-infrared optical regimes.; providing fluorescent probe scaffolds derived from biliverdin-binding photoreceptors
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They provide a route to fluorescent probe engineering using photoreceptor scaffolds suited to near-infrared optical regimes.
Source:
providing fluorescent probe scaffolds derived from biliverdin-binding photoreceptors
Problem links
providing fluorescent probe scaffolds derived from biliverdin-binding photoreceptors
LiteratureThey provide a route to fluorescent probe engineering using photoreceptor scaffolds suited to near-infrared optical regimes.
Source:
They provide a route to fluorescent probe engineering using photoreceptor scaffolds suited to near-infrared optical regimes.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Architecture: A reusable architecture pattern for arranging parts into an engineered system.
Techniques
No technique tags yet.
Target processes
recombinationInput: Light
Implementation Constraints
cofactor dependency: requires exogenous cofactorencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
Use depends on bacteriophytochrome-derived constructs and their interaction with biliverdin IXα.; requires bacteriophytochrome-derived designs and biliverdin cofactor compatibility
The abstract does not specify whether these probes solve issues such as maximal brightness, photostability, or minimal size.; the abstract does not specify individual probe families, brightness, or photophysical limitations
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Ideal absorption characteristics in the therapeutic near-infrared window and biliverdin availability in mammalian tissues have driven progress in re-engineering bacteriophytochromes for diverse applications.
Bacteriophytochromes preferentially interact with biliverdin IXα as an endogenous cofactor, which has enabled their use in optogenetic tools and fluorescent probe engineering.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug bacteriophytochrome-derived-fluorescent-probes
Considering their preferential interaction with biliverdin IXα as endogenous cofactor, they have recently been used for creating optogenetic tools and engineering fluorescent probes.
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application rationalesupports
Ideal absorption characteristics in the therapeutic near-infrared window and biliverdin availability in mammalian tissues have driven progress in re-engineering bacteriophytochromes for diverse applications.
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tool enabling propertysupports
Bacteriophytochromes preferentially interact with biliverdin IXα as an endogenous cofactor, which has enabled their use in optogenetic tools and fluorescent probe engineering.
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Comparisons
Source-stated alternatives
No explicit alternative fluorescent probe classes are named in the abstract.
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No explicit alternative fluorescent probe classes are named in the abstract.
Source-backed strengths
derived from systems with ideal absorption characteristics in the therapeutic near-infrared window; compatible with biliverdin as endogenous cofactor
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derived from systems with ideal absorption characteristics in the therapeutic near-infrared window
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compatible with biliverdin as endogenous cofactor
bacteriophytochrome-derived fluorescent probes and modular light-controlled skeletal muscle-powered bioactuator address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with Opto-Casp8-V2
bacteriophytochrome-derived fluorescent probes and Opto-Casp8-V2 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Compared with pcVP16
bacteriophytochrome-derived fluorescent probes and pcVP16 address a similar problem space because they share recombination.
Shared frame: same top-level item type; shared target processes: recombination; same primary input modality: light
Relative tradeoffs: looks easier to implement in practice; may avoid an exogenous cofactor requirement.
Ranked Citations
- 1.