Humanized mNeonGreen is a human codon-optimized cDNA construct encoding the fluorescent protein mNeonGreen for mammalian expression. In HEK293 cells, it produced higher fluorescent intensity than the original mNeonGreen, and derivative constructs supported anti-FLAG detection and mitochondrial targeting.
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Mechanism Concept
fluorescence
A mechanism-level grouping derived from the current toolkit evidence. Current coverage includes 4 architectures and 3 components. Across those tools, the main enabled capabilities are recombination. Representative components include Photoreceptor-derived biosensors, miRFP670nano3, and miRFP720.
7 total items4 architectures3 components
Main enabled capabilities: recombination.
Architectures
4 of 4a near-infrared rhodamine dye, prepared by photoirradiation and used as a fluorescent probe in cell imaging
Considering their preferential interaction with biliverdin IXα as endogenous cofactor, they have recently been used for creating optogenetic tools and engineering fluorescent probes.
Among them is the bimodal photoswitchable Neorhodopsin (NeoR), which exhibits a near-infrared absorbing, highly fluorescent state.
Components
3 of 3miRFP720 is a monomeric near-infrared fluorescent protein reported in the cited study as the most red-shifted monomeric NIR fluorescent protein. It functions as a fluorescent component for reporter construction that can be imaged with reduced spectral interference from visible-range probes and blue-green optogenetic tools.
Photoreceptor-derived biosensors are engineered protein tools built from natural light-sensing photoreceptors as molecular templates. The cited review presents natural photoreceptors as a broad source for developing biosensors, fluorescent proteins, and optogenetic tools responsive to light.
The paper introduces miRFP670nano3, a 17 kDa single-domain cyanobacteriochrome-derived near-infrared fluorescent protein.