Source 1primary paper2022
Toolkit/bow-knot-type gRNA
bow-knot-type gRNA
Taxonomy: Mechanism Branch / Component. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
The bow-knot-type gRNA is a light-responsive guide RNA format for CRISPR-Cas editing developed within a cyclically caged gRNA strategy. It is reported to enable optical control of gene editing, including light-mediated MSTN editing in embryos, while showing no background editing without light irradiation.
Usefulness & Problems
Why this is useful
This tool is useful for imposing temporal and spatial control over CRISPR-Cas editing through light input. The cited study presents it as an improved method for precise manipulation of where and when genes are edited, and the reported absence of background editing in the dark supports conditional activation.
Source:
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
Source:
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Problem solved
It addresses the problem of unwanted or poorly timed CRISPR-Cas activity by keeping the guide RNA inactive until light exposure. The available evidence specifically supports reduction of background editing in the absence of irradiation and light-triggered editing in an embryonic context.
Source:
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
Problem links
Need controllable genome or transcript editing
DerivedThe bow-knot-type gRNA is a light-responsive guide RNA format for CRISPR-Cas editing developed within a cyclically caged gRNA strategy. It is reported to enable optical control of gene editing, including light-mediated MSTN editing in embryos, while showing no background editing without light irradiation.
Need precise spatiotemporal control with light input
DerivedThe bow-knot-type gRNA is a light-responsive guide RNA format for CRISPR-Cas editing developed within a cyclically caged gRNA strategy. It is reported to enable optical control of gene editing, including light-mediated MSTN editing in embryos, while showing no background editing without light irradiation.
Taxonomy & Function
Primary hierarchy
Mechanism Branch
Component: A low-level RNA part used inside a larger architecture that realizes a mechanism.
Techniques
No technique tags yet.
Target processes
editingInput: Light
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator
The tool is described as a cyclically caged guide RNA and therefore involves covalent cyclization and chemical modification of the gRNA. Light irradiation is required for activation, but the provided evidence does not specify irradiation parameters, construct architecture, or delivery conditions.
The supplied evidence is limited to a single source and provides little quantitative performance detail beyond the no-background-editing claim and embryo MSTN editing example. The wavelength, photochemical group, editing efficiency, compatibility across Cas systems, and breadth of target validation are not specified in the provided evidence.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
Light-mediated MSTN gene editing was achieved in embryos.
We have also achieved light-mediated MSTN gene editing in embryos
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
The circular gRNA approach uses only two or three pre-installed photolabile substituents followed by simple covalent cyclization and is described as a more robust synthesis approach than heavily modified gRNAs.
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
Photo-sensitive circular gRNAs enable spatiotemporal and efficient CRISPR/Cas9- and Cpf1-mediated editing.
we reported a spatiotemporal and efficient CRISPR/Cas9 and Cpf1-mediated editing with photo-sensitive circular gRNAs
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, circular gRNA together with light irradiation directs precise cleavage of GFP and VEGFA within a pre-defined cutting region.
In established cells stably expressing Cas9, the circular gRNA in coordination with light irradiation could direct a precise cleavage of GFP and VEGFA within a pre-defined cutting region.
Approval Evidence
1 source2 linked approval claimsfirst-pass slug bow-knot-type-grna
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
Source:
application scopesupports
The method is presented as an improved way to precisely manipulate where and when genes are edited.
Together, our work provides a significantly improved method to precisely manipulate where and when genes are edited.
Source:
background activitysupports
A bow-knot-type gRNA showed no background editing in the absence of light irradiation.
a new bow-knot-type gRNA showed no background editing in the absence of light irradiation
Source:
Comparisons
Source-backed strengths
The main reported strength is that a bow-knot-type gRNA showed no background editing in the absence of light irradiation. The source also reports successful light-mediated MSTN gene editing in embryos, indicating that optical activation can function in a developmental setting.
Source:
This approach relies on only two or three pre-installed photolabile substituents followed by a simple covalent cyclization, which provides a robust synthesize approach in comparison to heavily modified gRNAs.
Compared with antisense oligonucleotides
bow-knot-type gRNA and antisense oligonucleotides address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
Compared with photo-sensitive circular gRNAs
bow-knot-type gRNA and photo-sensitive circular gRNAs address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
Compared with RNA aptamer
bow-knot-type gRNA and RNA aptamer address a similar problem space because they share editing.
Shared frame: same top-level item type; shared target processes: editing; same primary input modality: light
Ranked Citations
- 1.