Source 1review1997Journal of Biological Chemistry
Toolkit/ferricytochrome c superoxide detection assay
ferricytochrome c superoxide detection assay
Also known as: ferricytochrome c
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This has been circumvented by exploiting its reaction with various “detector” molecules such as ferricytochrome c...
Usefulness & Problems
Why this is useful
Ferricytochrome c is used as a detector molecule to infer superoxide through its reaction chemistry. The review presents it as a workaround for the instability of superoxide in solution.; detecting superoxide indirectly
Source:
Ferricytochrome c is used as a detector molecule to infer superoxide through its reaction chemistry. The review presents it as a workaround for the instability of superoxide in solution.
Source:
detecting superoxide indirectly
Problem solved
It helps detect superoxide when direct measurement is hindered by superoxide instability.; provides a way to detect or measure superoxide despite its instability in aqueous solution
Source:
It helps detect superoxide when direct measurement is hindered by superoxide instability.
Source:
provides a way to detect or measure superoxide despite its instability in aqueous solution
Problem links
provides a way to detect or measure superoxide despite its instability in aqueous solution
LiteratureIt helps detect superoxide when direct measurement is hindered by superoxide instability.
Source:
It helps detect superoxide when direct measurement is hindered by superoxide instability.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Mechanisms
redox reactionTechniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The method requires ferricytochrome c and, according to the review, SOD inhibition as a specificity control.; requires SOD inhibition control to lend specificity
It does not specifically distinguish superoxide from other reductants that can also reduce ferricytochrome c.; not specific for superoxide; other reductants can reduce ferricytochrome c
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Detector molecules such as ferricytochrome c and spin-trapping agents are used to detect superoxide, but they are not specific for superoxide and often rely on SOD inhibition to lend specificity.
Lucigenin luminescence is an inappropriate specific detector of superoxide because lucigenin chemistry can itself generate superoxide and increase intracellular superoxide production.
Luminol luminescence is misused as a superoxide measurement because the luminol radical can generate superoxide and the signal can be caused by multiple oxidants.
Nitroblue tetrazolium is an artifactual superoxide detector because tetrazolium radical intermediates can generate superoxide, making SOD-inhibitable signal possible even when superoxide was not initially present.
Rapid inactivation of [4Fe-4S]-containing dehydratases such as aconitase can be used as a reliable measure of intracellular superoxide, although other oxidants such as peroxynitrite can also inactivate aconitase.
Approval Evidence
1 source1 linked approval claimfirst-pass slug ferricytochrome-c-superoxide-detection-assay
This has been circumvented by exploiting its reaction with various “detector” molecules such as ferricytochrome c...
Source:
review summarysupports
Detector molecules such as ferricytochrome c and spin-trapping agents are used to detect superoxide, but they are not specific for superoxide and often rely on SOD inhibition to lend specificity.
Source:
Comparisons
Source-stated alternatives
The review contrasts ferricytochrome c with spin-trapping agents and with other luminescent detectors such as lucigenin and luminol.
Source:
The review contrasts ferricytochrome c with spin-trapping agents and with other luminescent detectors such as lucigenin and luminol.
Source-backed strengths
used as a detector molecule for superoxide
Source:
used as a detector molecule for superoxide
Compared with lucigenin luminescence assay
The review contrasts ferricytochrome c with spin-trapping agents and with other luminescent detectors such as lucigenin and luminol.
Shared frame: source-stated alternative in extracted literature
Strengths here: used as a detector molecule for superoxide.
Relative tradeoffs: not specific for superoxide; other reductants can reduce ferricytochrome c.
Source:
The review contrasts ferricytochrome c with spin-trapping agents and with other luminescent detectors such as lucigenin and luminol.
Compared with luminol luminescence assay
The review contrasts ferricytochrome c with spin-trapping agents and with other luminescent detectors such as lucigenin and luminol.
Shared frame: source-stated alternative in extracted literature
Strengths here: used as a detector molecule for superoxide.
Relative tradeoffs: not specific for superoxide; other reductants can reduce ferricytochrome c.
Source:
The review contrasts ferricytochrome c with spin-trapping agents and with other luminescent detectors such as lucigenin and luminol.
Compared with spin-trapping superoxide detection
The review contrasts ferricytochrome c with spin-trapping agents and with other luminescent detectors such as lucigenin and luminol.
Shared frame: source-stated alternative in extracted literature
Strengths here: used as a detector molecule for superoxide.
Relative tradeoffs: not specific for superoxide; other reductants can reduce ferricytochrome c.
Source:
The review contrasts ferricytochrome c with spin-trapping agents and with other luminescent detectors such as lucigenin and luminol.
Ranked Citations
- 1.