Source 1review1997Journal of Biological Chemistry
Toolkit/luminol luminescence assay
luminol luminescence assay
Also known as: luminol, luminol luminescence
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Another luminescent method that is misused to measure O·̄2 is based on luminol... Luminol luminescence thus can be caused by a variety of oxidants and in all cases SOD inhibits.
Usefulness & Problems
Why this is useful
Luminol luminescence is a light-emitting assay used in oxidant detection contexts. The review states that the luminol radical can itself generate superoxide before the light-emitting pathway proceeds.; luminescent detection of oxidant-dependent chemistry
Source:
Luminol luminescence is a light-emitting assay used in oxidant detection contexts. The review states that the luminol radical can itself generate superoxide before the light-emitting pathway proceeds.
Source:
luminescent detection of oxidant-dependent chemistry
Problem solved
It offers a luminescent readout in oxidant assays.; attempts to provide a luminescent readout for superoxide
Source:
It offers a luminescent readout in oxidant assays.
Source:
attempts to provide a luminescent readout for superoxide
Problem links
attempts to provide a luminescent readout for superoxide
LiteratureIt offers a luminescent readout in oxidant assays.
Source:
It offers a luminescent readout in oxidant assays.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The method requires luminol and luminescence detection, but its radical chemistry creates interpretability problems.; not specific for superoxide
It does not specifically measure superoxide because multiple oxidants can drive the signal and the detector can act as a superoxide source.; misused to measure superoxide; luminol radical can spontaneously reduce oxygen to superoxide; signal can be caused by a variety of oxidants
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Detector molecules such as ferricytochrome c and spin-trapping agents are used to detect superoxide, but they are not specific for superoxide and often rely on SOD inhibition to lend specificity.
Lucigenin luminescence is an inappropriate specific detector of superoxide because lucigenin chemistry can itself generate superoxide and increase intracellular superoxide production.
Luminol luminescence is misused as a superoxide measurement because the luminol radical can generate superoxide and the signal can be caused by multiple oxidants.
Nitroblue tetrazolium is an artifactual superoxide detector because tetrazolium radical intermediates can generate superoxide, making SOD-inhibitable signal possible even when superoxide was not initially present.
Rapid inactivation of [4Fe-4S]-containing dehydratases such as aconitase can be used as a reliable measure of intracellular superoxide, although other oxidants such as peroxynitrite can also inactivate aconitase.
Approval Evidence
1 source1 linked approval claimfirst-pass slug luminol-luminescence-assay
Another luminescent method that is misused to measure O·̄2 is based on luminol... Luminol luminescence thus can be caused by a variety of oxidants and in all cases SOD inhibits.
Source:
review summarysupports
Luminol luminescence is misused as a superoxide measurement because the luminol radical can generate superoxide and the signal can be caused by multiple oxidants.
Source:
Comparisons
Source-stated alternatives
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Source:
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Source-backed strengths
produces a luminescent signal in oxidant-containing systems
Source:
produces a luminescent signal in oxidant-containing systems
Compared with ferricytochrome c superoxide detection assay
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Shared frame: source-stated alternative in extracted literature
Strengths here: produces a luminescent signal in oxidant-containing systems.
Relative tradeoffs: misused to measure superoxide; luminol radical can spontaneously reduce oxygen to superoxide; signal can be caused by a variety of oxidants.
Source:
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Compared with lucigenin luminescence assay
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Shared frame: source-stated alternative in extracted literature
Strengths here: produces a luminescent signal in oxidant-containing systems.
Relative tradeoffs: misused to measure superoxide; luminol radical can spontaneously reduce oxygen to superoxide; signal can be caused by a variety of oxidants.
Source:
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Compared with nitroblue tetrazolium superoxide assay
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Shared frame: source-stated alternative in extracted literature
Strengths here: produces a luminescent signal in oxidant-containing systems.
Relative tradeoffs: misused to measure superoxide; luminol radical can spontaneously reduce oxygen to superoxide; signal can be caused by a variety of oxidants.
Source:
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Compared with spin-trapping superoxide detection
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Shared frame: source-stated alternative in extracted literature
Strengths here: produces a luminescent signal in oxidant-containing systems.
Relative tradeoffs: misused to measure superoxide; luminol radical can spontaneously reduce oxygen to superoxide; signal can be caused by a variety of oxidants.
Source:
The review compares it with lucigenin, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Ranked Citations
- 1.