Source 1review1997Journal of Biological Chemistry
Toolkit/spin-trapping superoxide detection
spin-trapping superoxide detection
Also known as: spin-trapping agents
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
This has been circumvented by exploiting its reaction with various “detector” molecules such as ferricytochrome c or spin-trapping agents.
Usefulness & Problems
Why this is useful
Spin-trapping agents are used to convert reactive species into EPR-detectable products. In this review they are discussed as indirect superoxide detectors.; detecting superoxide indirectly
Source:
Spin-trapping agents are used to convert reactive species into EPR-detectable products. In this review they are discussed as indirect superoxide detectors.
Source:
detecting superoxide indirectly
Problem solved
It offers a way to monitor superoxide-related chemistry despite the radical's instability.; provides a workaround for detecting unstable superoxide
Source:
It offers a way to monitor superoxide-related chemistry despite the radical's instability.
Source:
provides a workaround for detecting unstable superoxide
Problem links
provides a workaround for detecting unstable superoxide
LiteratureIt offers a way to monitor superoxide-related chemistry despite the radical's instability.
Source:
It offers a way to monitor superoxide-related chemistry despite the radical's instability.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The method requires spin-trapping reagents and EPR-compatible detection, with SOD inhibition used to improve specificity.; requires SOD inhibition control to lend specificity
It does not uniquely report superoxide because other oxidants can generate the detectable products.; not specific for superoxide; oxidants other than superoxide can convert spin traps to EPR-detectable hydroperoxy derivatives
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Detector molecules such as ferricytochrome c and spin-trapping agents are used to detect superoxide, but they are not specific for superoxide and often rely on SOD inhibition to lend specificity.
Lucigenin luminescence is an inappropriate specific detector of superoxide because lucigenin chemistry can itself generate superoxide and increase intracellular superoxide production.
Luminol luminescence is misused as a superoxide measurement because the luminol radical can generate superoxide and the signal can be caused by multiple oxidants.
Nitroblue tetrazolium is an artifactual superoxide detector because tetrazolium radical intermediates can generate superoxide, making SOD-inhibitable signal possible even when superoxide was not initially present.
Rapid inactivation of [4Fe-4S]-containing dehydratases such as aconitase can be used as a reliable measure of intracellular superoxide, although other oxidants such as peroxynitrite can also inactivate aconitase.
Approval Evidence
1 source1 linked approval claimfirst-pass slug spin-trapping-superoxide-detection
This has been circumvented by exploiting its reaction with various “detector” molecules such as ferricytochrome c or spin-trapping agents.
Source:
review summarysupports
Detector molecules such as ferricytochrome c and spin-trapping agents are used to detect superoxide, but they are not specific for superoxide and often rely on SOD inhibition to lend specificity.
Source:
Comparisons
Source-stated alternatives
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Source:
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Source-backed strengths
enables EPR-detectable derivatives
Source:
enables EPR-detectable derivatives
Compared with ferricytochrome c superoxide detection assay
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables EPR-detectable derivatives.
Relative tradeoffs: not specific for superoxide; oxidants other than superoxide can convert spin traps to EPR-detectable hydroperoxy derivatives.
Source:
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Compared with lucigenin luminescence assay
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables EPR-detectable derivatives.
Relative tradeoffs: not specific for superoxide; oxidants other than superoxide can convert spin traps to EPR-detectable hydroperoxy derivatives.
Source:
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Compared with luminol luminescence assay
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables EPR-detectable derivatives.
Relative tradeoffs: not specific for superoxide; oxidants other than superoxide can convert spin traps to EPR-detectable hydroperoxy derivatives.
Source:
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Compared with nitroblue tetrazolium superoxide assay
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Shared frame: source-stated alternative in extracted literature
Strengths here: enables EPR-detectable derivatives.
Relative tradeoffs: not specific for superoxide; oxidants other than superoxide can convert spin traps to EPR-detectable hydroperoxy derivatives.
Source:
The review mentions ferricytochrome c, lucigenin luminescence, luminol luminescence, nitroblue tetrazolium, and aconitase-based measurement as alternatives or contrasts.
Ranked Citations
- 1.