Source 1review1997Journal of Biological Chemistry
Toolkit/lucigenin luminescence assay
lucigenin luminescence assay
Also known as: lucigenin, lucigenin luminescence
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
An example is the luminescence that can be elicited from lucigenin... the use of lucigenin as a “specific” detector of O·̄2 continues... lucigenin was shown to function, much as does paraquat, to increase intracellular O·̄2 production.
Usefulness & Problems
Why this is useful
Lucigenin luminescence is used as a luminescent detector in superoxide studies. The review emphasizes that its chemistry requires prior reduction and can itself lead to superoxide generation.; luminescent detection of superoxide-related chemistry
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Lucigenin luminescence is used as a luminescent detector in superoxide studies. The review emphasizes that its chemistry requires prior reduction and can itself lead to superoxide generation.
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luminescent detection of superoxide-related chemistry
Problem solved
It aims to provide a convenient luminescent measure of superoxide-related activity.; attempts to provide a luminescent readout for superoxide
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It aims to provide a convenient luminescent measure of superoxide-related activity.
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attempts to provide a luminescent readout for superoxide
Problem links
attempts to provide a luminescent readout for superoxide
LiteratureIt aims to provide a convenient luminescent measure of superoxide-related activity.
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It aims to provide a convenient luminescent measure of superoxide-related activity.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
recombinationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The assay requires lucigenin and a luminescence readout system, but the review stresses that the reagent's own redox chemistry is a major confounder.; not suitable as a specific intracellular superoxide detector based on the review's critique
It does not provide a specific measure of intracellular superoxide because lucigenin can itself produce superoxide and perturb the system being measured.; misused as a specific detector of superoxide; lucigenin can generate superoxide through autoxidation of its monocation radical; can increase intracellular superoxide production
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Detector molecules such as ferricytochrome c and spin-trapping agents are used to detect superoxide, but they are not specific for superoxide and often rely on SOD inhibition to lend specificity.
Lucigenin luminescence is an inappropriate specific detector of superoxide because lucigenin chemistry can itself generate superoxide and increase intracellular superoxide production.
Luminol luminescence is misused as a superoxide measurement because the luminol radical can generate superoxide and the signal can be caused by multiple oxidants.
Nitroblue tetrazolium is an artifactual superoxide detector because tetrazolium radical intermediates can generate superoxide, making SOD-inhibitable signal possible even when superoxide was not initially present.
Rapid inactivation of [4Fe-4S]-containing dehydratases such as aconitase can be used as a reliable measure of intracellular superoxide, although other oxidants such as peroxynitrite can also inactivate aconitase.
Approval Evidence
1 source1 linked approval claimfirst-pass slug lucigenin-luminescence-assay
An example is the luminescence that can be elicited from lucigenin... the use of lucigenin as a “specific” detector of O·̄2 continues... lucigenin was shown to function, much as does paraquat, to increase intracellular O·̄2 production.
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review summarysupports
Lucigenin luminescence is an inappropriate specific detector of superoxide because lucigenin chemistry can itself generate superoxide and increase intracellular superoxide production.
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Comparisons
Source-stated alternatives
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
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The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Source-backed strengths
produces a luminescent signal linked to superoxide-related chemistry
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produces a luminescent signal linked to superoxide-related chemistry
Compared with ferricytochrome c superoxide detection assay
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Shared frame: source-stated alternative in extracted literature
Strengths here: produces a luminescent signal linked to superoxide-related chemistry.
Relative tradeoffs: misused as a specific detector of superoxide; lucigenin can generate superoxide through autoxidation of its monocation radical; can increase intracellular superoxide production.
Source:
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Compared with luminol luminescence assay
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Shared frame: source-stated alternative in extracted literature
Strengths here: produces a luminescent signal linked to superoxide-related chemistry.
Relative tradeoffs: misused as a specific detector of superoxide; lucigenin can generate superoxide through autoxidation of its monocation radical; can increase intracellular superoxide production.
Source:
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Compared with nitroblue tetrazolium superoxide assay
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Shared frame: source-stated alternative in extracted literature
Strengths here: produces a luminescent signal linked to superoxide-related chemistry.
Relative tradeoffs: misused as a specific detector of superoxide; lucigenin can generate superoxide through autoxidation of its monocation radical; can increase intracellular superoxide production.
Source:
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Compared with spin-trapping superoxide detection
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Shared frame: source-stated alternative in extracted literature
Strengths here: produces a luminescent signal linked to superoxide-related chemistry.
Relative tradeoffs: misused as a specific detector of superoxide; lucigenin can generate superoxide through autoxidation of its monocation radical; can increase intracellular superoxide production.
Source:
The review contrasts lucigenin with luminol, nitroblue tetrazolium, ferricytochrome c, spin-trapping agents, and aconitase-based measurement.
Ranked Citations
- 1.