Toolkit/lyso-ChR2

lyso-ChR2

Construct Pattern·Research·Since 2024

Taxonomy: Mechanism Branch / Architecture. Workflows sit above the mechanism and technique branches rather than replacing them.

Summary

Here, we constructed lysosome-localized optogenetic actuators, named lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2, to achieve optogenetic manipulation of lysosomes. Notably, lyso-ChR2 activation induces autophagy through the mTOR pathway, promotes Aβ clearance in an autophagy-dependent manner in cellular models, and alleviates Aβ-induced paralysis in the Caenorhabditis elegans model of Alzheimer's disease.

Usefulness & Problems

Why this is useful

lyso-ChR2 is a lysosome-localized optogenetic actuator used to manipulate lysosomal physiology with light. The abstract further states that its activation induces autophagy through the mTOR pathway and promotes amyloid beta clearance.; optogenetic manipulation of lysosomes; inducing autophagy through the mTOR pathway; promoting Aβ clearance; testing lysosome-directed intervention in cellular and animal disease models

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lyso-ChR2 is a lysosome-localized optogenetic actuator used to manipulate lysosomal physiology with light. The abstract further states that its activation induces autophagy through the mTOR pathway and promotes amyloid beta clearance.

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optogenetic manipulation of lysosomes

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inducing autophagy through the mTOR pathway

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promoting Aβ clearance

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testing lysosome-directed intervention in cellular and animal disease models

Problem solved

It provides a dynamic way to regulate lysosomal physiology and to test how lysosomal activation affects autophagy and amyloid beta handling.; lack of methods to dynamically regulate lysosomal function in living cells or animals; need for a dynamic method to probe links between lysosomal physiology, autophagy, and amyloid beta clearance

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It provides a dynamic way to regulate lysosomal physiology and to test how lysosomal activation affects autophagy and amyloid beta handling.

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lack of methods to dynamically regulate lysosomal function in living cells or animals

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need for a dynamic method to probe links between lysosomal physiology, autophagy, and amyloid beta clearance

Problem links

lack of methods to dynamically regulate lysosomal function in living cells or animals

Literature

It provides a dynamic way to regulate lysosomal physiology and to test how lysosomal activation affects autophagy and amyloid beta handling.

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It provides a dynamic way to regulate lysosomal physiology and to test how lysosomal activation affects autophagy and amyloid beta handling.

need for a dynamic method to probe links between lysosomal physiology, autophagy, and amyloid beta clearance

Literature

It provides a dynamic way to regulate lysosomal physiology and to test how lysosomal activation affects autophagy and amyloid beta handling.

Source:

It provides a dynamic way to regulate lysosomal physiology and to test how lysosomal activation affects autophagy and amyloid beta handling.

Published Workflows

Optogenetic manipulation of lysosomal physiology and autophagy-dependent clearance of amyloid beta.

2024

Objective: Construct lysosome-localized optogenetic actuators to dynamically regulate lysosomal physiology and test whether lysosomal optical control can drive autophagy and amyloid beta clearance in living cells and animals.

Why it works: The paper's logic is that lysosome-localized optogenetic actuators allow dynamic, light-dependent perturbation of lysosomal physiological parameters, enabling causal testing of how lysosomal state affects autophagy and amyloid beta clearance.

optical control of lysosomal physiologyautophagy induction through the mTOR pathwayautophagy-dependent Aβ clearanceconstruction of lysosome-localized optogenetic actuatorslight-dependent perturbation in living cells and animals

Stages

  1. 1.
    Construction of lysosome-localized optogenetic actuators(library_build)

    This stage creates the engineered tools needed for dynamic optical perturbation of lysosomal function.

    Selection: Build lysosome-localized actuators lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2 for optogenetic manipulation of lysosomes.

  2. 2.
    Functional characterization of lysosomal physiological control in living cells(functional_characterization)

    This stage establishes that the engineered actuators actually modulate lysosomal physiology in living cells before disease-relevant applications are tested.

    Selection: Test whether the new actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.

  3. 3.
    Application testing of lyso-ChR2 in autophagy and amyloid beta clearance(confirmatory_validation)

    This stage tests whether lysosomal optical control translates into mechanistic and disease-relevant cellular outcomes.

    Selection: Use lyso-ChR2 to test autophagy induction through the mTOR pathway and autophagy-dependent Aβ clearance in cellular models.

  4. 4.
    In vivo validation in a Caenorhabditis elegans Alzheimer's disease model(in_vivo_validation)

    This stage extends cellular findings to an animal disease model.

    Selection: Test whether lyso-ChR2 alleviates Aβ-induced paralysis in a Caenorhabditis elegans model of Alzheimer's disease.

Taxonomy & Function

Primary hierarchy

Mechanism Branch

Architecture: A reusable architecture pattern for arranging parts into an engineered system.

Techniques

No technique tags yet.

Target processes

degradation

Input: Light

Implementation Constraints

cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationimplementation constraint: spectral hardware requirementoperating role: regulator

Use requires expression of a lysosome-localized optogenetic construct and light stimulation in living cells or animals.; requires light activation; requires lysosome-localized actuator expression

Needs compatible illumination hardware and optical access. Independent follow-up evidence is still limited. Validation breadth across biological contexts is still narrow. Independent reuse still looks limited, so the evidence base may be fragile. No canonical validation observations are stored yet, so context-specific performance remains under-specified.

Validation

Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication

Supporting Sources

Ranked Claims

Claim 1application effectsupports2024Source 1needs review

lyso-ChR2 activation promotes Aβ clearance in an autophagy-dependent manner in cellular models.

promotes Aβ clearance in an autophagy-dependent manner in cellular models
Claim 2application effectsupports2024Source 1needs review

lyso-ChR2 alleviates Aβ-induced paralysis in the Caenorhabditis elegans model of Alzheimer's disease.

and alleviates Aβ-induced paralysis in the Caenorhabditis elegans model of Alzheimer's disease
Claim 3mechanistic effectsupports2024Source 1needs review

lyso-ChR2 activation induces autophagy through the mTOR pathway.

Notably, lyso-ChR2 activation induces autophagy through the mTOR pathway
Claim 4tool capabilitysupports2024Source 1needs review

lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2 are lysosome-localized optogenetic actuators constructed to achieve optogenetic manipulation of lysosomes.

Here, we constructed lysosome-localized optogenetic actuators, named lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2, to achieve optogenetic manipulation of lysosomes.
Claim 5tool capabilitysupports2024Source 1needs review

The lysosome-localized optogenetic actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.

These new actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.
Claim 6tool use casesupports2024Source 1needs review

Lysosomal optogenetic actuators provide a method to dynamically regulate lysosomal physiology and function in living cells and animals.

Our lysosomal optogenetic actuators supplement the optogenetic toolbox and provide a method to dynamically regulate lysosomal physiology and function in living cells and animals.

Approval Evidence

1 source6 linked approval claimsfirst-pass slug lyso-chr2
Here, we constructed lysosome-localized optogenetic actuators, named lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2, to achieve optogenetic manipulation of lysosomes. Notably, lyso-ChR2 activation induces autophagy through the mTOR pathway, promotes Aβ clearance in an autophagy-dependent manner in cellular models, and alleviates Aβ-induced paralysis in the Caenorhabditis elegans model of Alzheimer's disease.

Source:

application effectsupports

lyso-ChR2 activation promotes Aβ clearance in an autophagy-dependent manner in cellular models.

promotes Aβ clearance in an autophagy-dependent manner in cellular models

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application effectsupports

lyso-ChR2 alleviates Aβ-induced paralysis in the Caenorhabditis elegans model of Alzheimer's disease.

and alleviates Aβ-induced paralysis in the Caenorhabditis elegans model of Alzheimer's disease

Source:

mechanistic effectsupports

lyso-ChR2 activation induces autophagy through the mTOR pathway.

Notably, lyso-ChR2 activation induces autophagy through the mTOR pathway

Source:

tool capabilitysupports

lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2 are lysosome-localized optogenetic actuators constructed to achieve optogenetic manipulation of lysosomes.

Here, we constructed lysosome-localized optogenetic actuators, named lyso-NpHR3.0, lyso-ArchT, and lyso-ChR2, to achieve optogenetic manipulation of lysosomes.

Source:

tool capabilitysupports

The lysosome-localized optogenetic actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.

These new actuators enable light-dependent control of lysosomal membrane potential, pH, hydrolase activity, degradation, and Ca2+ dynamics in living cells.

Source:

tool use casesupports

Lysosomal optogenetic actuators provide a method to dynamically regulate lysosomal physiology and function in living cells and animals.

Our lysosomal optogenetic actuators supplement the optogenetic toolbox and provide a method to dynamically regulate lysosomal physiology and function in living cells and animals.

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Comparisons

Source-stated alternatives

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

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The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Source-backed strengths

lysosome-localized optogenetic actuator; linked in the abstract to autophagy induction through the mTOR pathway; associated with Aβ clearance in cellular models; associated with alleviation of Aβ-induced paralysis in a Caenorhabditis elegans Alzheimer's disease model

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lysosome-localized optogenetic actuator

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linked in the abstract to autophagy induction through the mTOR pathway

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associated with Aβ clearance in cellular models

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associated with alleviation of Aβ-induced paralysis in a Caenorhabditis elegans Alzheimer's disease model

Compared with lyso-ArchT

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Shared frame: source-stated alternative in extracted literature

Strengths here: lysosome-localized optogenetic actuator; linked in the abstract to autophagy induction through the mTOR pathway; associated with Aβ clearance in cellular models.

Source:

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Compared with lyso-NpHR3.0

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Shared frame: source-stated alternative in extracted literature

Strengths here: lysosome-localized optogenetic actuator; linked in the abstract to autophagy induction through the mTOR pathway; associated with Aβ clearance in cellular models.

Source:

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Compared with optogenetic

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Shared frame: source-stated alternative in extracted literature

Strengths here: lysosome-localized optogenetic actuator; linked in the abstract to autophagy induction through the mTOR pathway; associated with Aβ clearance in cellular models.

Source:

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Compared with optogenetic actuator

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Shared frame: source-stated alternative in extracted literature

Strengths here: lysosome-localized optogenetic actuator; linked in the abstract to autophagy induction through the mTOR pathway; associated with Aβ clearance in cellular models.

Source:

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Compared with optogenetic actuators

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Shared frame: source-stated alternative in extracted literature

Strengths here: lysosome-localized optogenetic actuator; linked in the abstract to autophagy induction through the mTOR pathway; associated with Aβ clearance in cellular models.

Source:

The same paper presents lyso-NpHR3.0 and lyso-ArchT as related lysosomal optogenetic actuators.

Ranked Citations

  1. 1.
    StructuralSource 1MED2024Claim 1Claim 2Claim 3

    Extracted from this source document.