Source 1primary paper2025MED
Toolkit/next-generation sequencing
next-generation sequencing
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
Multi-locus sequencing, multiplex PCR, and next-generation sequencing refined the identification of fungal and bacterial pathogens.
Usefulness & Problems
Why this is useful
Next-generation sequencing is described as refining identification of fungal and bacterial pathogens in chrysanthemum.; identification of fungal pathogens in chrysanthemum; identification of bacterial pathogens in chrysanthemum; NGS is described as an advanced tool that helps identify novel tumor-specific targets. The abstract also links it to improving therapy design.; identifying novel tumor-specific targets; improving therapy designs; The abstract lists next-generation sequencing as one of the latest technologies enabling sensitive tobamovirus detection and enhanced surveillance capabilities.; tobamovirus detection; surveillance
Source:
Next-generation sequencing is described as refining identification of fungal and bacterial pathogens in chrysanthemum.
Source:
identification of fungal pathogens in chrysanthemum
Source:
identification of bacterial pathogens in chrysanthemum
Source:
NGS is described as an advanced tool that helps identify novel tumor-specific targets. The abstract also links it to improving therapy design.
Source:
identifying novel tumor-specific targets
Source:
improving therapy designs
Source:
The abstract lists next-generation sequencing as one of the latest technologies enabling sensitive tobamovirus detection and enhanced surveillance capabilities.
Source:
tobamovirus detection
Source:
surveillance
Problem solved
It improves pathogen identification in chrysanthemum disease studies.; refines pathogen identification; insufficient target identification for improved CAR-T design; It contributes to surveillance and detection of emerging tobamoviruses.; supports sensitive detection and surveillance of tobamoviruses
Source:
It improves pathogen identification in chrysanthemum disease studies.
Source:
refines pathogen identification
Source:
insufficient target identification for improved CAR-T design
Source:
It contributes to surveillance and detection of emerging tobamoviruses.
Source:
supports sensitive detection and surveillance of tobamoviruses
Problem links
insufficient target identification for improved CAR-T design
LiteratureNGS is described as an advanced tool that helps identify novel tumor-specific targets. The abstract also links it to improving therapy design.
Source:
NGS is described as an advanced tool that helps identify novel tumor-specific targets. The abstract also links it to improving therapy design.
refines pathogen identification
LiteratureIt improves pathogen identification in chrysanthemum disease studies.
Source:
It improves pathogen identification in chrysanthemum disease studies.
supports sensitive detection and surveillance of tobamoviruses
LiteratureIt contributes to surveillance and detection of emerging tobamoviruses.
Source:
It contributes to surveillance and detection of emerging tobamoviruses.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Target processes
No target processes tagged yet.
Implementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract supports a sequencing-based identification role but does not provide platform, library-prep, or analysis details.; requires sequencing capability; The abstract does not specify sequencing platform, library preparation, or analysis requirements.
The abstract does not claim that next-generation sequencing itself controls disease or creates resistance.
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Future sustainable chrysanthemum protection should integrate molecular breeding, genome editing, RNA-based tools, and microbiome management.
Multi-locus sequencing, multiplex PCR, and next-generation sequencing refined identification of fungal and bacterial pathogens in chrysanthemum research.
Approval Evidence
4 sources3 linked approval claimsfirst-pass slug next-generation-sequencing
Sensitive detection of tobamoviruses in the field with minimal sample preparation can be achieved using latest technologies such as isothermal amplification, CRISPR/Cas-hybrid assays or next-generation sequencing.
Source:
advanced tools such as next-generation sequencing (NGS), direct stochastic optical reconstruction microscopy (dSTORM), or multiparametric flow cytometry are helping to identify novel tumor-specific targets and improve therapy designs
Source:
Multi-locus sequencing, multiplex PCR, and next-generation sequencing refined the identification of fungal and bacterial pathogens.
Source:
advances in next-generation sequencing (NGS) and gene-editing technology have begun to close this gap
Source:
capabilitysupports
Isothermal amplification, CRISPR/Cas-hybrid assays, and next-generation sequencing can achieve sensitive detection of tobamoviruses in the field with minimal sample preparation.
Source:
method applicationsupports
Multi-locus sequencing, multiplex PCR, and next-generation sequencing refined identification of fungal and bacterial pathogens in chrysanthemum research.
Source:
use casesupports
NGS, dSTORM, and multiparametric flow cytometry help identify novel tumor-specific targets and improve CAR-T therapy designs.
advanced tools such as next-generation sequencing (NGS), direct stochastic optical reconstruction microscopy (dSTORM), or multiparametric flow cytometry are helping to identify novel tumor-specific targets and improve therapy designs
Source:
Comparisons
Source-stated alternatives
The abstract mentions multi-locus sequencing and multiplex PCR as alternative identification approaches.; The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Source:
The abstract mentions multi-locus sequencing and multiplex PCR as alternative identification approaches.
Source:
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Source-backed strengths
presented as improving pathogen identification; presented as an advanced tool supporting target discovery; included among latest technologies that enhanced surveillance capabilities
Source:
presented as improving pathogen identification
Source:
presented as an advanced tool supporting target discovery
Source:
included among latest technologies that enhanced surveillance capabilities
Compared with assays
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as improving pathogen identification; presented as an advanced tool supporting target discovery; included among latest technologies that enhanced surveillance capabilities.
Source:
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Compared with CRISPR/Cas9
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as improving pathogen identification; presented as an advanced tool supporting target discovery; included among latest technologies that enhanced surveillance capabilities.
Source:
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Compared with CRISPR/Cas9 system
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as improving pathogen identification; presented as an advanced tool supporting target discovery; included among latest technologies that enhanced surveillance capabilities.
Source:
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Compared with CRISPR/Cas-hybrid assays
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as improving pathogen identification; presented as an advanced tool supporting target discovery; included among latest technologies that enhanced surveillance capabilities.
Source:
The abstract mentions isothermal amplification and CRISPR/Cas-hybrid assays as alternative diagnostic approaches.
Compared with multiplex PCR
The abstract mentions multi-locus sequencing and multiplex PCR as alternative identification approaches.
Shared frame: source-stated alternative in extracted literature
Strengths here: presented as improving pathogen identification; presented as an advanced tool supporting target discovery; included among latest technologies that enhanced surveillance capabilities.
Source:
The abstract mentions multi-locus sequencing and multiplex PCR as alternative identification approaches.
Ranked Citations
- 1.