Source 1primary paper2023Preprints.org
Toolkit/STED
STED
Also known as: STED microscopy
Taxonomy: Technique Branch / Method. Workflows sit above the mechanism and technique branches rather than replacing them.
Summary
we have super-resolution microscopy techniques such as STED, PALM and STORM able to achieve 10 to 20 nm spatial resolution in single molecule localisation
Usefulness & Problems
Why this is useful
STED is presented as a super-resolution microscopy technique used for high-spatial-resolution protein imaging and single-molecule localization. In the abstract it is grouped with PALM and STORM as reaching 10 to 20 nm spatial resolution.; super-resolution imaging; single-molecule localization with high spatial resolution
Source:
STED is presented as a super-resolution microscopy technique used for high-spatial-resolution protein imaging and single-molecule localization. In the abstract it is grouped with PALM and STORM as reaching 10 to 20 nm spatial resolution.
Source:
super-resolution imaging
Source:
single-molecule localization with high spatial resolution
Problem solved
It helps resolve subcellular protein localization at much finer spatial scale than conventional imaging.; improves spatial resolution for protein imaging at subcellular scale
Source:
It helps resolve subcellular protein localization at much finer spatial scale than conventional imaging.
Source:
improves spatial resolution for protein imaging at subcellular scale
Problem links
improves spatial resolution for protein imaging at subcellular scale
LiteratureIt helps resolve subcellular protein localization at much finer spatial scale than conventional imaging.
Source:
It helps resolve subcellular protein localization at much finer spatial scale than conventional imaging.
Taxonomy & Function
Primary hierarchy
Technique Branch
Method: A concrete measurement method used to characterize an engineered system.
Techniques
Functional AssayTarget processes
localizationImplementation Constraints
cofactor dependency: cofactor requirement unknownencoding mode: genetically encodedimplementation constraint: context specific validationoperating role: sensor
The abstract indicates dependence on suitable fluorophores and practical limits from spectral overlap. It is discussed in the context of live-cell imaging and multi-colour imaging constraints.; practical implementation is hampered by fluorophore limitations and spectral overlap
The abstract says current approaches still suffer from slow image acquisition and therefore limited high-temporal-resolution tracking of multiple protein targets in live cells.; slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging
Validation
Cell-freeBacteriaMammalianMouseHumanTherapeuticIndep. Replication
Supporting Sources
Ranked Claims
Slow image acquisition in current super-resolution microscopy limits high temporal resolution tracking of multiple protein targets in live-cell imaging.
STED, PALM, and STORM can achieve 10 to 20 nm spatial resolution in single-molecule localization.
spatial resolution 10 to 20 nm
Approval Evidence
1 source2 linked approval claimsfirst-pass slug sted
we have super-resolution microscopy techniques such as STED, PALM and STORM able to achieve 10 to 20 nm spatial resolution in single molecule localisation
Source:
limitation statementsupports
Slow image acquisition in current super-resolution microscopy limits high temporal resolution tracking of multiple protein targets in live-cell imaging.
Source:
performance statementsupports
STED, PALM, and STORM can achieve 10 to 20 nm spatial resolution in single-molecule localization.
Source:
Comparisons
Source-stated alternatives
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Source-backed strengths
reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution
Source:
reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution
Compared with microscopy
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution.
Relative tradeoffs: slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Compared with PALM
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution.
Relative tradeoffs: slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Compared with photoactivated localization microscopy
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution.
Relative tradeoffs: slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Compared with photo-activation localization microscopy
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution.
Relative tradeoffs: slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Compared with photoactivation localization microscopy
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution.
Relative tradeoffs: slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Compared with stochastic optical reconstruction microscopy
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution.
Relative tradeoffs: slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Compared with STORM
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution.
Relative tradeoffs: slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Compared with super-resolution microscopy
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Shared frame: source-stated alternative in extracted literature
Strengths here: reported alongside PALM and STORM as able to achieve 10 to 20 nm spatial resolution.
Relative tradeoffs: slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging.
Source:
PALM and STORM are named as alternative super-resolution microscopy techniques in the same sentence.
Ranked Citations
- 1.